Results 21 to 30 of about 279,791 (361)

Molecular diagnosis of the human immunodeficiency, Hepatitis B and C viruses among blood donors in Lomé (Togo) by multiplex real time PCR

The Pan African Medical Journal, 2016
This study aimed to compare the sensitivity of multiplex PCR to ELISA technique in the instantaneous detection of HBV, HCV and HIVin blood samples from donors of the National blood Transfusion Centre in Togo.
Maleki Assih, Lochina Feteke, Cyrille Bisseye, Djeneba Ouermi, Florencia Djigma, Simplice Damintoti Karou, Jacques Simpore   +6 more
doaj   +1 more source

Fragment Size Difference between Multiplex and Singleplex PCR Products and Their Practical Implications

BioTechniques, 2000
By simultaneously amplifying several loci in the same reaction, multiplex PCR has been used in gene mapping and DNA typing with polymorphic short tandem repeat loci.
H.-W. Deng, Y. Zhou, R.R. Recker, M.L. Johnson, J. Li   +4 more
doaj   +1 more source

primerJinn: a tool for rationally designing multiplex PCR primer sets for amplicon sequencing and performing in silico PCR

BMC Bioinformatics, 2023
Background Multiplex PCR amplifies numerous targets in a single tube reaction and is essential in molecular biology and clinical diagnostics. One of its most important applications is in the targeted sequencing of pathogens.
Jason D. Limberis, John Z. Metcalfe
doaj   +1 more source

Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (Juglans regia L.)

Silva Fennica, 2016
Multiplex polymerase chain reaction (PCR) allows amplification of two or more pair of primers in parallel for amplification of multiple target sequences in a single reaction tube.
Nevenka Ćelepirović, Monika Karija Vlahović, Aikaterini Dounavi, Mladen Ivanković   +3 more
doaj   +1 more source

PCR múltiplex para el diagnóstico de microorganismos atípicos transmitidos sexualmente

Perinatología y Reproducción Humana, 2023
Antecedentes: Las infecciones de transmisión sexual son un problema de salud pública mundial. El análisis rutinario incluye solo pruebas microbiológicas y serológicas para el diagnóstico de patógenos.
Marcos R. Escobedo-Guerra, Marcela López-Hurtado, Rodrigo Gutiérrez-Trujillo, Abraham D. Bustos-López, Fernando M. Guerra-Infante   +4 more
doaj   +1 more source

Development of novel multiplex microsatellite polymerase chain reactions to enable high-throughput population genetic studies of Schistosoma haematobium [PDF]

, 2015
© 2015 Webster et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in ...
A. A. Hamidou, A. Garba, A. Gouvras, A. M. Emery, AM Emery, B. L. Webster, BL Webster, C Van Oosterhout, CM Gower, CM Gower, D. Rollinson, F. Allan, J. P. Webster, JP Webster, K. A. Mohammed, L Meurs, M Kearse, M. Rabone, R Peakall, S Knopp, S. Knopp, S. M. Ame, T. Pennance, TC Glenn   +23 more
core   +4 more sources

Analysis of a novel multiplex polymerase chain reaction assay as a sensitive tool for the diagnosis of indeterminate and tuberculoid forms of leprosy

International Journal of Mycobacteriology, 2017
Objective/Background: Clinical diagnosis of indeterminate and tuberculoid leprosy is often difficult due to limited and confounding signs and symptoms. In the current study, we evaluated the utility of new multiplex polymerase chain reaction (PCR) using
V Sundeep Chaitanya, Luz Cuello, Madhusmita Das, A Sudharsan, Pavithrra Ganesan, K Kanmani, Lakshmi Rajan, Mannam Ebenezer   +7 more
doaj   +1 more source

Multiplex PCR assay based on the citE2 gene and intergenic sequence for the rapid detection of Salmonella Pullorum in chickens

Poultry Science, 2022
: Salmonella is one of the most common Gram-negative pathogens and seriously threatens chicken farms and food safety. This study aimed to establish a multiplex polymerase chain reaction (PCR) approach for the identification of different Salmonella ...
Zhike Liu, Yan Yu, Tetiana Fotina, Roman Petrov, Zhanna Klishchova, Anatoliy Fotin, Jinyou Ma   +6 more
doaj   +1 more source

Occurrence of Pectinatus and Megasphaera in the major UK breweries [PDF]

, 2011
The occurrence of beer spoilage bacteria belonging to the genera Pectinatus and Megasphaera in ten major UK breweries was investigated. The sampling points were selected from fermentation areas, beer conditioning areas and beer bottling and canning sites.
Hill, Anne Elizabeth, Mitchell, Wilfrid James, Paradh, A D   +2 more
core   +1 more source

Multiplex PCR for identifying mycobacterial isolates. [PDF]

Journal of Clinical Pathology, 1995
Aims-To develop a multiplex polymerase chain reaction (PCR) method to facilitate identification of mycobacterial isolates. Methods-Type strains of 14 species of mycobacteria and 56 clinical isolates were lysed by boiling in TE Triton. The lysate (5 mu 1) was used directly in a PCR reaction incorporating three pairs of PCR primers expected to amplify ...
Cormican, M, Glennon, M, Riain, U N, Flynn, J   +3 more
openaire   +3 more sources