Results 21 to 30 of about 281,013 (338)

primerJinn: a tool for rationally designing multiplex PCR primer sets for amplicon sequencing and performing in silico PCR

BMC Bioinformatics, 2023
Background Multiplex PCR amplifies numerous targets in a single tube reaction and is essential in molecular biology and clinical diagnostics. One of its most important applications is in the targeted sequencing of pathogens.
Jason D. Limberis, John Z. Metcalfe
doaj   +1 more source

Development of a Multiplex Crystal Digital RT-PCR for Differential Detection of Classical, Highly Pathogenic, and NADC30-like Porcine Reproductive and Respiratory Syndrome Virus

Animals, 2023
Porcine reproductive and respiratory syndrome virus (PRRSV) type 1 (European genotype) and PRRSV type 2 (North American genotype) are prevalent all over the world.
Feng Long, Yating Chen, Kaichuang Shi, Yanwen Yin, Shuping Feng, Hongbin Si   +5 more
doaj   +1 more source

Diversity of the parB and repA genes of the Burkholderia cepacia complex and their utility for rapid identification of Burkholderia cenocepacia [PDF]

, 2008
Background: Burkholderia cenocepacia is the most prominent species of the B. cepacia complex (Bcc), a group of nine closely related and difficult to identify bacteria that cause serious infections in patients with cystic fibrosis.
Baldwin, Adam, Dowson, Christopher G., Drevinek, Pavel, Mahenthiralingam, Eshwar   +3 more
core   +5 more sources

Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (Juglans regia L.)

Silva Fennica, 2016
Multiplex polymerase chain reaction (PCR) allows amplification of two or more pair of primers in parallel for amplification of multiple target sequences in a single reaction tube.
Nevenka Ćelepirović, Monika Karija Vlahović, Aikaterini Dounavi, Mladen Ivanković   +3 more
doaj   +1 more source

PCR múltiplex para el diagnóstico de microorganismos atípicos transmitidos sexualmente

Perinatología y Reproducción Humana, 2023
Antecedentes: Las infecciones de transmisión sexual son un problema de salud pública mundial. El análisis rutinario incluye solo pruebas microbiológicas y serológicas para el diagnóstico de patógenos.
Marcos R. Escobedo-Guerra, Marcela López-Hurtado, Rodrigo Gutiérrez-Trujillo, Abraham D. Bustos-López, Fernando M. Guerra-Infante   +4 more
doaj   +1 more source

Development of novel multiplex microsatellite polymerase chain reactions to enable high-throughput population genetic studies of Schistosoma haematobium [PDF]

, 2015
© 2015 Webster et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in ...
A. A. Hamidou, A. Garba, A. Gouvras, A. M. Emery, AM Emery, B. L. Webster, BL Webster, C Van Oosterhout, CM Gower, CM Gower, D. Rollinson, F. Allan, J. P. Webster, JP Webster, K. A. Mohammed, L Meurs, M Kearse, M. Rabone, R Peakall, S Knopp, S. Knopp, S. M. Ame, T. Pennance, TC Glenn   +23 more
core   +4 more sources

Analysis of a novel multiplex polymerase chain reaction assay as a sensitive tool for the diagnosis of indeterminate and tuberculoid forms of leprosy

International Journal of Mycobacteriology, 2017
Objective/Background: Clinical diagnosis of indeterminate and tuberculoid leprosy is often difficult due to limited and confounding signs and symptoms. In the current study, we evaluated the utility of new multiplex polymerase chain reaction (PCR) using
V Sundeep Chaitanya, Luz Cuello, Madhusmita Das, A Sudharsan, Pavithrra Ganesan, K Kanmani, Lakshmi Rajan, Mannam Ebenezer   +7 more
doaj   +1 more source

Multiplex PCR for identifying mycobacterial isolates. [PDF]

Journal of Clinical Pathology, 1995
Aims-To develop a multiplex polymerase chain reaction (PCR) method to facilitate identification of mycobacterial isolates. Methods-Type strains of 14 species of mycobacteria and 56 clinical isolates were lysed by boiling in TE Triton. The lysate (5 mu 1) was used directly in a PCR reaction incorporating three pairs of PCR primers expected to amplify ...
Cormican, M, Glennon, M, Riain, U N, Flynn, J   +3 more
openaire   +3 more sources

Evaluation of multiplex ligation-dependent probe amplification analysis versus multiplex polymerase chain reaction assays in the detection of dystrophin gene rearrangements in an Iranian population subset

Advanced Biomedical Research, 2014
Background: The Duchenne muscular dystrophy (DMD) gene is located in the short arm of the X chromosome (Xp21). It spans 2.4 Mb of the human genomic DNA and is composed of 79 exons.
Nayereh Nouri, Esmat Fazel-Najafabadi, Mansoor Salehi, Majid Hosseinzadeh, Mahdieh Behnam, Mohammad Reza Ghazavi, Maryam Sedghi   +6 more
doaj   +1 more source

Multiplex PCR assay based on the citE2 gene and intergenic sequence for the rapid detection of Salmonella Pullorum in chickens

Poultry Science, 2022
: Salmonella is one of the most common Gram-negative pathogens and seriously threatens chicken farms and food safety. This study aimed to establish a multiplex polymerase chain reaction (PCR) approach for the identification of different Salmonella ...
Zhike Liu, Yan Yu, Tetiana Fotina, Roman Petrov, Zhanna Klishchova, Anatoliy Fotin, Jinyou Ma   +6 more
doaj   +1 more source