Occurrence of Pectinatus and Megasphaera in the major UK breweries [PDF]
The occurrence of beer spoilage bacteria belonging to the genera Pectinatus and Megasphaera in ten major UK breweries was investigated. The sampling points were selected from fermentation areas, beer conditioning areas and beer bottling and canning sites.
Hill, Anne Elizabeth +2 more
core +1 more source
Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi [PDF]
Background: Within the last years, numerous reports described successful application of the CRISPR nucleases Cas9 and Cpf1 for genome editing in filamentous fungi.
Haefner, Stefan +4 more
core +1 more source
Introduction: Staphylococcus aureus, coagulase-negative staphylococci, Enterococcus spp., Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumanii have been found to be the most prevalent bacteremia-causing bacteria in patients with ...
Mohammad Reza Arabestani +2 more
doaj +1 more source
Multiple Locus Variable number of tandem repeat Analysis : a molecular genotyping tool for Paenibacillus larvae [PDF]
American Foulbrood, caused by Paenibacillus larvae, is the most severe bacterial disease of honey bees (Apis mellifera). To perform genotyping of P.larvae in an epidemiological context, there is a need of a fast and cheap method with a high resolution ...
Alippi +40 more
core +2 more sources
PCR for the detection of pathogens in neonatal early onset sepsis. [PDF]
BACKGROUND: A large proportion of neonates are treated for presumed bacterial sepsis with broad spectrum antibiotics even though their blood cultures subsequently show no growth. This study aimed to investigate PCR-based methods to identify pathogens not
Bedford Russell, A +8 more
core +1 more source
Real-Time PCR and Multiplex Approaches [PDF]
Analysis of RNA expression levels by real-time reverse-transcription (RT) PCR has become a routine technique in diagnostic and research laboratories. Monitoring of DNA amplification can be done using fluorescent sequence-specific probes, which generate signal only upon binding to their target.
Olga L, Gurvich, Mikhail, Skoblov
openaire +2 more sources
Peroxisome Proliferator-Activated Receptor alpha (PPAR alpha) down-regulation in cystic fibrosis lymphocytes [PDF]
Background: PPARs exhibit anti-inflammatory capacities and are potential modulators of the inflammatory response. We hypothesized that their expression and/or function may be altered in cystic fibrosis (CF), a disorder characterized by an excessive host ...
A Augarten +53 more
core +4 more sources
Regional Differences in Presence of Shiga toxin-producing Escherichia coli Virulence-Associated Genes in the Environment in the North West and East Anglian regions of England [PDF]
Shiga toxin-producing Escherichia coli is carried in the intestine of ruminant animals, and outbreaks have occurred after contact with ruminant animals or their environment.
Hunter, Paul R +6 more
core +1 more source
A method for genotyping elite breeding stocks of leaf chicory (Cichorium intybus L.) by assaying mapped microsatellite marker loci [PDF]
BACKGROUND: Leaf chicory (Cichorium intybus subsp. intybus var. foliosum L.) is a diploid plant species (2n = 18) of the Asteraceae family. The term "chicory" specifies at least two types of cultivated plants: a leafy vegetable, which is highly ...
Barcaccia, Gianni +5 more
core +2 more sources
Establishment of multiplex PCR for detection of genes related to Quinolone resistance
Background/aims: It has been shown that quinolone resistance arises due to mutations in the quinolone resistance-determining regions of the drug targets.
Ngo Tat Trung +4 more
doaj +1 more source

