Results 141 to 150 of about 465,638 (353)

“Stat” Multiplex Polymerase Chain Reaction [PDF]

open access: yesAmerican Journal of Clinical Pathology, 2013
openaire   +1 more source

Droplet Digital CRISPR for Nucleic Acid Detection

open access: yesAdvanced Science, EarlyView.
This review outlines recent advances in droplet digital CRISPR technology for nucleic acid detection, combining CRISPR specificity with droplet digital‐based absolute quantification. It summarizes core principles, amplification‐assisted and amplification‐free strategies, and representative DNA and RNA biomarker applications.
Yang Zhang   +4 more
wiley   +1 more source

A Modular DNAzyme for Precise Visualization and Intervention of Alternative Splicing Isoforms in Live Cells

open access: yesAdvanced Science, EarlyView.
A Stringent dUPlex‐activated Error Robust (SUPER) DNAzyme system enables real‐time imaging of alternative mRNA splicing (e.g., Bcl‐xL/Bcl‐xS) in living cells via target‐triggered split‐DNAzyme reassembly and dual‐color fluorescence. It also achieves mRNA‐selective knockdown through DNAzyme‐based gene regulation, serving as a versatile tool for splicing
Mengru Lin   +6 more
wiley   +1 more source

Detection of genomic mutations in katG, inhA and rpoB genes of Mycobacterium tuberculosis isolates using polymerase chain reaction and multiplex allele-specific polymerase chain reaction

open access: yesBrazilian Journal of Infectious Diseases
OBJECTIVE: Isoniazid (INH) and rifampin (RIF) are the most effective first line antibiotics against Mycobacterium tuberculosis. Mutations in several genes determine resistance of M.
Azar Dokht Khosravi   +2 more
doaj   +1 more source

Comparative Evaluation of Diatherix Target Enriched Multiplex Polymerase Chain Reaction and BioFire FilmArray in the Detection of Viral and Bacterial Respiratory Pathogens [PDF]

open access: gold, 2016
Wei‐Ju Chen   +11 more
openalex   +1 more source

Microenvironment‐Guided Evolution of ssDNA‐SWCNT Probes for Selective Recognition of Aggressive Prostate Cancer Phenotypes

open access: yesAdvanced Science, EarlyView.
Random ssDNA–SWCNT(single strand DNA‐single walled carbon nanotube) library is functionally screened and evolved using high‐throughput 3D prostate tumor spheroids via iterative positive and negative selection, enabling the identification of NIR‐II fluorescent ssDNA‐SWCNT nanoprobe specific for prostate cancer.
Dakyeon Lee   +8 more
wiley   +1 more source

Nanozymes Integrated Biochips Toward Smart Detection System

open access: yesAdvanced Science, EarlyView.
This review systematically outlines the integration of nanozymes, biochips, and artificial intelligence (AI) for intelligent biosensing. It details how their convergence enhances signal amplification, enables portable detection, and improves data interpretation.
Dongyu Chen   +10 more
wiley   +1 more source

Impact of Multiplex Polymerase Chain Reaction Test in Patients With Meningitis or Encephalitis. [PDF]

open access: yesOpen Forum Infect Dis, 2023
Kitagawa D   +19 more
europepmc   +1 more source

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