Results 161 to 170 of about 129,024 (210)

Diagnosing superinfection keratitis with multiplex polymerase chain reaction

Journal of Infection and Chemotherapy, 2018
To report the potential usefulness of multiplex polymerase chain reaction (mPCR) for diagnosing superinfection keratitis caused by herpes simplex virus-1 (HSV-1), bacteria and fungus.Case series. Corneal scrapings were analyzed with mPCR for human herpes virus 1-8, bacterial 16S ribosomal DNA (rDNA) and fungal 28S rDNA.Case 1 was a 69-year-old man who ...
Masaaki, Yoshida, Takehiro, Hariya, Shunji, Yokokura, Kazuichi, Maruyama, Kota, Sato, Sunao, Sugita, Yasuhiro, Tomaru, Norio, Shimizu, Toru, Nakazawa   +8 more
openaire   +2 more sources

Multiplex polymerase chain reaction assay for genotyping Clostridium perfringens

American Journal of Veterinary Research, 1997
Abstract Objective To develop a multiplex polymerase chain reaction (PCR) assay to detect the genes for the major toxins of Clostridium perfringens (cpa [α toxin], cpb [β toxin], etx [ε toxin], iA [ι i toxin], and cpe [enterotoxin]). Sample Population Cultures of C perfringens obtained from collections and diagnosticians throughout North America ...
R R, Meer, J G, Songer
openaire   +2 more sources

Multiplex gene removal by two-step polymerase chain reactions

Analytical Biochemistry, 2015
Precise DNA manipulation is critical for molecular biotechnology. Restriction enzyme-based approaches are limited by their requirement of specific enzyme sites. Restriction-free cloning has greatly improved the flexibility and speed of precise DNA assembly. Most of these approaches focus on DNA assembly rather than gene removal.
Vishnu Vardhan, Krishnamurthy, John S, Khamo, Ellen, Cho, Cara, Schornak, Kai, Zhang   +4 more
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Human sex determination using multiplex polymerase chain reaction (PCR)

Science & Justice, 1996
A sex determination method has been developed using the polymerase chain reaction (PCR) which involved the amplification of the sex-determining region Y (SRY) gene and the amplification of the HLA-DQa gene as an internal control. This method, which can be applied to old and degraded DNA samples, allowed confident sexing of biological samples producing ...
Finch, Jennie Louise, Hope, Rory M., van Daal, A.   +2 more
openaire   +3 more sources

Multiplexed digital polymerase chain reaction as a powerful diagnostic tool

Biosensors and Bioelectronics, 2021
The digital polymerase chain reaction (dPCR) multiplexing method can simultaneously detect and quantify closely related deoxyribonucleic acid sequences in complex mixtures. The dPCR concept is continuously improved by the development of microfluidics and micro- and nanofabrication, and different complex techniques are introduced.
Martina Gaňová, Haoqing Zhang, Hanliang Zhu, Marie Korabečná, Pavel Neužil   +4 more
openaire   +2 more sources

Multiplex polymerase chain reaction of Alu polymorphic insertions

ELECTROPHORESIS, 1998
AbstractAlu sequences are present in humans in excess of 500 000 copies per haploid genome and represent the largest family of short interspersed repetitive elements (SINEs). These mobile genetic elements are ancestrally derived from the 7SL RNA gene and move throughout the genomes of primates by retroposition. Polymorphic Alu insertions have proven to
E, Thomas, R J, Herrera
openaire   +2 more sources