Results 81 to 90 of about 465,638 (353)

Role of multiplex polymerase chain reaction using IS6110 and Protein b for the diagnosis of extra-pulmonary tuberculosis: North India.

Indian Journal of Pathology and Microbiology, 2015
BACKGROUND Prompt and accurate diagnosis of extra-pulmonary tuberculosis (TB) is highly challenging. Current conventional techniques lack sensitivity and are time-consuming.
K. Sharma, S. Appannanavar, M. Modi, Malkit Singh, Aman Sharma, S. Varma   +5 more
semanticscholar   +1 more source

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp [PDF]

Journal of Visualized Experiments, 2018
The cerebral cortex is composed of numerous cell types exhibiting various morphological, physiological, and molecular features. This diversity hampers easy identification and characterization of these cell types, prerequisites to study their specific functions.
Devienne, Gabrielle, Le Gac, Benjamin, Piquet, Juliette, Cauli, Bruno   +3 more
openaire   +3 more sources

High‐Throughput In Vivo Screening Using Barcoded mRNA Identifies Lipid Nanoparticles With Extrahepatic Tropism for In Situ Immunoengineering

Advanced Materials, EarlyView.
A high‐throughput in vivo mRNA LNP screening platform is developed and employed to screen a large library of 122 mRNA LNPs in vivo for delivery to immune, stromal, and parenchymal cells, identifying promising LNP candidates. A novel small particle flow cytometry‐based protein adsorption analysis method is utilized to interrogate protein corona ...
Alex G. Hamilton, Ajay S. Thatte, Junchao Xu, Zhangyi Luo, Hannah C. Safford, Kelsey L. Swingle, Jenna Muscat‐Rivera, Michael Kegel, Xuexiang Han, Ryann A. Joseph, Amanda M. Murray, Hannah C. Geisler, Ricardo C. Whitaker, Lulu Xue, Roman Spektor, Jilian R. Melamed, Drew Weissman, Michael J. Mitchell   +17 more
wiley   +1 more source

DNA Origami‐Templated Aptamer Chiral Structures Realize Cellular Enantioselectivity

Advanced Materials, EarlyView.
Aptamers displayed on tubular DNA origami nanostructures in defined chiral orientations (left‐ or right‐handed) exhibit enantioselective interactions with cell surface target proteins. While the right‐handed configuration supports only transient binding, the left handedness energetically favors protein dimerization and promotes subsequent ...
Tingjie Song, Abhisek Dwivedy, Dhanush Gandavadi, Gayatri Chandran, Yiquan An, Mengxi Zheng, Xiaojing Wang, Lifeng Zhou, Yang Zhao, Xing Wang   +9 more
wiley   +1 more source

Multiomics Analyses Reveal an Essential Role of Tryptophan in Treatment of csDMARDs in Rheumatoid Arthritis

Advanced Science, EarlyView.
Rheumatoid arthritis is a disease characterized by joint inflammation. Approximately 50% of patients show insufficient response to traditional synthetic disease‐modifying antirheumatic drugs. This study aims to elucidate differential molecular profiles of the mechanisms underlying drug responses through multi‐omics strategy.
Congcong Jian, Jing Zhu, Jianhong Wu, Yan Zhang, Jianghua Chen, Huan Wang, Hengyan Liu, Ke Xu, Jiaxin Huang, Xiaoting Zhu, Yuanli Wei, Shilin Li, Tingting Wang, Xuan Huang, Qinghua Zou, Jie Zhang, Jiang Su, Xinming Du, Yaping Lu, Tianci Zhou, Yingtong Zhou, Minglong Tang, Bin Li, Xue Zhou, Qihao Wei, Qiulong Yan, Fanxin Zeng   +26 more
wiley   +1 more source

Positive real-time PCR in pneumococcal meningitis 12 hours after initiation of antibiotic therapy – case report

BMC Neurology
Background Purulent meningitis poses a significant clinical challenge with high mortality. We present the case of a 54-year-old female transferred to our emergency department with suspected bacterial meningitis, later diagnosed as an Austrian syndrome ...
Cem Thunstedt, Carla Palleis, Johannes Wischmann, Suzette Heck, Konstantinos Dimitriadis, Matthias Klein   +5 more
doaj   +1 more source

Simultaneous detection of major blackleg and soft rot bacterial pathogens in potato by multiplex polymerase chain reaction

Annals of Applied Biology, 2014
A multiplex polymerase chain reaction (PCR) assay for simultaneous, fast and reliable detection of the main soft rot and blackleg potato pathogens in Europe has been developed.
M. Potrykus, W. Sledz, Małgorzata Golanowska, M. Slawiak, A. Binek, Agata Motyka, Sabina Żołędowska, R. Czajkowski, E. Lojkowska   +8 more
semanticscholar   +1 more source

Cryopreserved Human Otic Neuronal Spheroids Self‐assemble for Functional Connectivity Analysis and Long‐term Ototoxicity Evaluation

Advanced Science, EarlyView.
This study establishes a robust and reproducible protocol for generating human otic neuronal spheroids (hONS) from cryopreserved hPSC‐derived pre‐placodal ectoderm cells. These hONS further differentiate into functional SGN‐like neurons, which extend neurite projections toward both murine hair cells and human cortical organoids, thereby forming ...
Gaoying Sun, Yukai Wang, Man Wang, Xinyue Wang, Mingming Tang, Da Li, Jianhuan Qi, Xue Wang, Shujuan Sun, Lei Chen, Weibin An, Ligang Kong, Anqi Suo, Haibo Wang, Wenwen Liu, Lei Xu   +15 more
wiley   +1 more source

NDST3‐Induced Epigenetic Reprogramming Reverses Neurodegeneration in Parkinson's Disease

Advanced Science, EarlyView.
NDST3‐mediated epigenetic reprogramming revitalizes neuronal circuits in the substantia nigra and striatum to halt dopaminergic neuron degeneration and restore motor function in Parkinson's disease models. This strategy promotes neuronal maintenance and functional recovery, highlighting NDST3's therapeutic potential in neurodegenerative disorders ...
Yujung Chang, Yongwoo Na, Hyeonjoo Im, Garam Yang, Seungseon Yang, Hyun Soo Shim, Chunggoo Kim, GyeungYun Kim, Hyeok Ju Park, Hee Young Kim, Seung Eun Lee, Wonwoong Lee, Yoon Ha, Sungho Park, Jieun Kim, Won‐Young Cho, Woong Sun, Jong‐Seo Kim, Junsang Yoo   +18 more
wiley   +1 more source

Screening for Y Chromosome Microdeletion in a Nonobstructive Azoospermic Male Patient with Allogeneic Bone Marrow Transplantation from His Sister

Case Reports in Medicine, 2010
Genomic DNA of a patient diagnosed with nonobstructive azoospermia and with the history of allogenic bone marrow transplantation from his sister due to chronic myeloid leukemia was isolated from peripheral blood in order to screen Y chromosome ...
Hakan Gurkan, Faruk Kucukdurmaz, Tolga Akman, Filiz Aydın, Ates Kadioglu   +4 more
doaj   +1 more source