Results 81 to 90 of about 129,024 (210)

Ex vivo innate immune cytokine signature of enhanced risk of relapsing brucellosis. [PDF]

, 2013
BackgroundBrucellosis, a zoonotic infection caused by one of the Gram-negative intracellular bacteria of the Brucella genus, is an ongoing public health problem in Perú.
Feldman, Kristyn E, Gilman, Robert H, Gotuzzo, Eduardo, Hoffmann, Alexander, Loriaux, Paul M, Saito, Mayuko, Siva, Tenaya, Tuero, Iskra, Villaverde, Homarh, Vinetz, Joseph M   +9 more
core   +3 more sources

Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (Juglans regia L.)

Silva Fennica, 2016
Multiplex polymerase chain reaction (PCR) allows amplification of two or more pair of primers in parallel for amplification of multiple target sequences in a single reaction tube.
Nevenka Ćelepirović, Monika Karija Vlahović, Aikaterini Dounavi, Mladen Ivanković   +3 more
doaj   +1 more source

Comparison of prevalence estimates of pfhrp2 and pfhrp3 deletions in Plasmodium falciparum determined by conventional PCR and multiplex qPCR and implications for surveillance and monitoring

International Journal of Infectious Diseases
Objectives: The accuracy of malaria rapid diagnostic tests is threatened by Plasmodium falciparum with pfhrp2/3 deletions. This study compares gene deletion prevalence determined by multiplex real time polymerase chain reaction (qPCR) and conventional ...
Michelle L. Gatton, David Smith, Cielo Pasay, Karen Anderson, Selam Mihreteab, Hugo O. Valdivia, Juan F. Sanchez, Khalid B. Beshir, Jane Cunningham, Qin Cheng   +9 more
doaj   +1 more source

Digital PCR methods improve detection sensitivity and measurement precision of low abundance mtDNA deletions [PDF]

, 2016
Mitochondrial DNA (mtDNA) mutations are a common cause of primary mitochondrial disorders, and have also been implicated in a broad collection of conditions, including aging, neurodegeneration, and cancer.
A Bender, C Barthélémy, CT Moraes, CT Moraes, DC Wallace, DW Dong, Ea Schon, EJ Brierley, G Campbell, J Damas, J Damas, J Villarroya, J Wanagat, JB Stewart, JP Grady, K Khrapko, K Maniura-Weber, KJ Krishnan, L He, L Van Haute, L-JJC Wong, LC Greaves, M Elstner, M Sciacco, NF Cariello, NG Gokey, NR Phillips, NZ Lax, P Manoj, PF Chinnery, RW Taylor, S DiMauro, SD Taylor, SSL Chan, V Nesbitt, X Guo, Y Kraytsberg, Y Tang, Z Cao   +38 more
core   +1 more source

Development of Sybr Green I-Based Melting Curve Method for HER2I655V Polymorphism Detection in Breast Cancer [PDF]

, 2016
Background: Currently available molecular method to detect HER2I655V polymorphism such as PCR-RFLP is hampered by the costly experimental method, and post-PCR treatment requirement that makes this technique is not meeting for high-throughput analysis ...
Budiarto, B. R. (Bugi), Desriani, D. (Desriani), Harahap, W. A. (Wirsma)   +2 more
core   +3 more sources

Performance of diagnostic tests to detect respiratory viruses in older adults. [PDF]

, 2010
The performance of 4 laboratory methods for diagnosis of viral respiratory tract infections (RTI) in older adults was evaluated. Seventy-four nasopharyngeal (NP) swab specimens were obtained from 60 patients with RTI at a long-term care facility over 2 ...
Caram, Lauren B, Hymas, Weston C, Petti, Cathy A, Polage, Christopher R, Schmader, Kenneth, She, Rosemary C, Taggart, Edward W, Woods, Christopher W   +7 more
core  

Identification of Salmonella enteritidis isolates by polymerase chain reaction and multiplex polymerase chain reaction.

Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi, 2003
The polymerase chain reaction and the multiplex polymerase chain reaction were developed for detection of Salmonella and for identification of the serotype enteritidis. Three sets of primers were selected from different genomic sequences amplifying a 429 bp fragment specific for the genus Salmonella within a randomly cloned sequence, including a 250 bp
Tzu-Ming, Pan, Yi-Ju, Liu
openaire   +1 more source

Molecular typing of Brucella species in human brucellosis cases from Eastern Türkiye

Revista Científica
Brucellosis remains a critical zoonotic infection with profound implications for public health across diverse regions, including the Middle East, Asia, the Arabian Peninsula, the Mediterranean, Africa, and South and Central American countries.
İbrahim Halil Şahin, Sevil Erdenliğ Gürbilek, Nida Özcan, Ahmet Murat Saytekin, Ayfer Güllü Yücetepe   +4 more
doaj   +1 more source

Sex identification of turkey embryos using a multiplex polymerase chain reaction

Poultry Science, 1998
A considerable portion of the W chromosome in Gallinaceous birds consists of tandem repetitive DNA. In the turkey, a 0.4-kb PstI element is repeated about 10,000 times in the female diploid genome but is undetectable as such a unit in males. In this study a multiplex polymerase chain reaction was developed to identify the sex of turkeys based upon the ...
S, D'Costa, J N, Petitte
openaire   +2 more sources

Enhanced Specificity of Multiplex Polymerase Chain Reaction via CdTe Quantum Dots

Nanoscale Research Letters, 2011
Nanoparticles were recently reported to be able to improve both efficiency and specificity in polymerase chain reaction (PCR). Here, CdTe QDs were introduced into multi-PCR systems. It was found that an appropriate concentration of CdTe QDs could enhance
Liang Gaofeng, Ma Chao, Zhu Yanliang, Li Shuchun, Shao Youhua, Wang Yong, Xiao Zhongdang   +6 more
doaj