Revisiting the Endocytosis of the M2 Muscarinic Acetylcholine Receptor [PDF]
Membranes, 2015 The agonist-induced endocytosis of the muscarinic acetylcholine receptor M2 is different from that of the other members of the muscarinic receptor family.
Wymke Ockenga, Ritva Tikkanen
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Acetylcholine muscarinic M2 receptor maintains human Schwann-like adipose-derived phenotype in the absence of differentiating medium [PDF]
Cell Death DiscoveryHuman adipose-derived stem cells (ASCs), differentiated in vitro towards Schwann-like phenotype (hdASCs), have been suggested as an alternative source of Schwann cells (SCs).
Roberta Piovesana +3 more
doaj +3 more sources
Trafficking of M2 Muscarinic Acetylcholine Receptors [PDF]
Journal of Biological Chemistry, 1999 Internalization is an important mechanism regulating the agonist-dependent responses of G-protein-coupled receptors. The internalization of the M(2) muscarinic cholinergic receptors (mAChR) in HEK293 cells has been demonstrated to occur by an unknown mechanism that is independent of arrestins and dynamin.
Aaron G. Roseberry +1 more
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Expression of Functional M2 Muscarinic Acetylcholine Receptor in Escherichia coli [PDF]
Journal of Biochemistry, 2000 The M2 muscarinic acetylcholine receptor mutant (M2 mutant), with a lack of glycosylation sites, a deletion in the central part of the third inner loop, and the addition of a six histidine tag at the C-terminus, was fused to maltose binding protein (MBP) at its N-terminus and expressed in Escherichia coli. The expression level was 0.2 nmol receptor per
Hiroyasu Furukawa, Tatsuya Haga
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Notch Signal Mediates the Cross-Interaction between M2 Muscarinic Acetylcholine Receptor and Neuregulin/ErbB Pathway: Effects on Schwann Cell Proliferation [PDF]
Biomolecules, 2022 The cross-talk between axon and glial cells during development and in adulthood is mediated by several molecules. Among them are neurotransmitters and their receptors, which are involved in the control of myelinating and non-myelinating glial cell ...
Roberta Piovesana +5 more
doaj +2 more sources
Modulation of the M2 Muscarinic Acetylcholine Receptor Activity with Monoclonal Anti-M2 Receptor Antibody Fragments [PDF]
Journal of Biological Chemistry, 2004 Antibodies directed against the second extracellular loop of G protein-coupled receptors are known to have functional activities. From a partial agonist monoclonal antibody directed against the M2 muscarinic receptor, we constructed and produced a single chain variable fragment with high affinity for its target epitope.
Jean‐Christophe Peter +6 more
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Structure of the human M2 muscarinic acetylcholine receptor bound to an antagonist [PDF]
Nature, 2012 The parasympathetic branch of the autonomic nervous system regulates the activity of multiple organ systems. Muscarinic receptors are G-protein-coupled receptors that mediate the response to acetylcholine released from parasympathetic nerves. Their role in the unconscious regulation of organ and central nervous system function makes them potential ...
Kazuko Haga +10 more
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Pronounced pharmacologic deficits in M2 muscarinic acetylcholine receptor knockout mice [PDF]
Proceedings of the National Academy of Sciences, 1999 Members of the muscarinic acetylcholine receptor family (M1–M5) are known to be involved in a great number of important central and peripheral physiological and pathophysiological processes. Because of the overlapping expression patterns of the M1–M5 muscarinic receptor subtypes and the lack of ligands endowed with sufficient subtype selectivity ...
Jesús Gomeza +8 more
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Downstream Signaling of Muscarinic M4 Receptors Is Regulated by Receptor Density and Cellular Environment [PDF]
Pharmacology Research &Perspectives, Volume 13, Issue 3, June 2025.ABSTRACT Multiple muscarinic M4 receptor modulators are currently advancing in clinical development for the treatment of positive symptoms in schizophrenia, including agonists and positive allosteric modulators. Considering the importance of comprehending M4 receptor pharmacology for these therapeutic applications, this study investigates M4 receptor ...
Madeleine Merz +5 more
wiley +2 more sources
Light‐Activated Pharmacological Tools for Exploring the Cholinergic System [PDF]
Medicinal Research Reviews, Volume 45, Issue 4, Page 1251-1274, July 2025.ABSTRACT Cholinergic transmission plays a critical role in both the central and peripheral nervous systems, affecting processes such as learning, memory, and inflammation. Conventional cholinergic drugs generally suffer from poor selectivity and temporal precision, leading to undesired effects and limited therapeutic efficacy. Photopharmacology aims to
Alessio Colleoni +5 more
wiley +2 more sources