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We need to talk—how muscle stem cells communicate [PDF]

Frontiers in Cell and Developmental Biology
Skeletal muscle is one of the tissues with the highest ability to regenerate, a finely controlled process which is critically depending on muscle stem cells.
Karolina Majchrzak, Erik Hentschel, Katja Hönzke, Katja Hönzke, Christiane Geithe, Julia von Maltzahn, Julia von Maltzahn, Julia von Maltzahn   +7 more
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Protocols to generate and isolate mouse myogenic progenitors both in vitro and in vivo

STAR Protocols, 2023
Summary: Mouse embryonic stem cells (mESCs) can be directed to acquire cell-lineage-specific genetic programs and phenotypes by stepwise exposure to defined factors, allowing the development of in vitro models for studying disease and tissue generation ...
Mamduh Khateb, Xuesong Feng, Stefania Dell’Orso, Vittorio Sartorelli   +3 more
doaj   +1 more source

Integrating single-cell transcriptomes, chromatin accessibility, and multiomics analysis of mesoderm-induced embryonic stem cells

STAR Protocols, 2023
Summary: Here, we present workflows for integrating independent transcriptomic and chromatin accessibility datasets and analyzing multiomics. First, we describe steps for integrating independent transcriptomic and chromatin accessibility measurements ...
Kyung Dae Ko, Kan Jiang, Stefania Dell’Orso, Vittorio Sartorelli   +3 more
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A miR-375/YAP axis regulates neuroendocrine differentiation and tumorigenesis in lung carcinoid cells

Scientific Reports, 2021
Lung carcinoids are variably aggressive and mechanistically understudied neuroendocrine neoplasms (NENs). Here, we identified and elucidated the function of a miR-375/yes-associated protein (YAP) axis in lung carcinoid (H727) cells.
Xiaojing Yang, Jina Nanayakkara, Duncan Claypool, Sadegh Saghafinia, Justin J. M. Wong, Minqi Xu, Xiantao Wang, Christopher J. B. Nicol, Iacovos P. Michael, Markus Hafner, Xiaolong Yang, Neil Renwick   +11 more
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NEDD4-1 deficiency impairs satellite cell function during skeletal muscle regeneration

Biological Research, 2023
Background Satellite cells are tissue-specific stem cells primarily responsible for the regenerative capacity of skeletal muscle. Satellite cell function and maintenance are regulated by extrinsic and intrinsic mechanisms, including the ubiquitin ...
Felipe Cabezas, Claudio Cabello-Verrugio, Natalia González, Jeremy Salas, Manuel J. Ramírez, Eduardo de la Vega, Hugo C. Olguín   +6 more
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Multiple capsid protein binding sites mediate selective packaging of the alphavirus genomic RNA

Nature Communications, 2020
Alphaviruses need to selectively package genomic viral RNA for transmission, but the packaging mechanism remains unclear. Here, Brown et al. combine PAR-CLIP with biotinylated capsid protein (Cp) retrieval and identify multiple Cp binding sites on ...
Rebecca S. Brown, Dimitrios G. Anastasakis, Markus Hafner, Margaret Kielian   +3 more
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METTL3-Mediated m6A Methylation Regulates Muscle Stem Cells and Muscle Regeneration by Notch Signaling Pathway

Stem Cells International, 2021
The Pax7+ muscle stem cells (MuSCs) are essential for skeletal muscle homeostasis and muscle regeneration upon injury, while the molecular mechanisms underlying muscle stem cell fate determination and muscle regeneration are still not fully understood ...
Yu Liang, Hui Han, Qiuchan Xiong, Chunlong Yang, Lu Wang, Jieyi Ma, Shuibin Lin, Yi-Zhou Jiang   +7 more
doaj   +1 more source

Protocol for RNA-seq library preparation starting from a rare muscle stem cell population or a limited number of mouse embryonic stem cells

STAR Protocols, 2021
Summary: It remains challenging to generate reproducible, high-quality cDNA libraries from RNA derived from rare cell populations. Here, we describe a protocol for high-throughput RNA-seq library preparation, including isolation of 200 skeletal muscle ...
Stefania Dell’Orso, Aster H. Juan, Victoria Moiseeva, Laura García-Prat, Pura Muñoz-Cánoves, Vittorio Sartorelli   +5 more
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Muscle stem cell adaptations to cellular and environmental stress

Skeletal Muscle, 2022
Background Lifelong regeneration of the skeletal muscle is dependent on a rare population of resident skeletal muscle stem cells, also named ‘satellite cells’ for their anatomical position on the outside of the myofibre and underneath the basal lamina ...
Maria Vittoria Gugliuzza, Colin Crist
doaj   +1 more source

The Histone Variant MacroH2A1.2 Is Necessary for the Activation of Muscle Enhancers and Recruitment of the Transcription Factor Pbx1

Cell Reports, 2016
Summary: Histone variants complement and integrate histone post-translational modifications in regulating transcription. The histone variant macroH2A1 (mH2A1) is almost three times the size of its canonical H2A counterpart, due to the presence of an ∼25 ...
Stefania Dell’Orso, A. Hongjun Wang, Han-Yu Shih, Kayoko Saso, Libera Berghella, Gustavo Gutierrez-Cruz, Andreas G. Ladurner, John J. O’Shea, Vittorio Sartorelli, Hossein Zare   +9 more
doaj   +1 more source