Forward and Reverse Genetics of B Cell Malignancies: From Insertional Mutagenesis to CRISPR-Cas
Frontiers in Immunology, 2021 Cancer genome sequencing has identified dozens of mutations with a putative role in lymphomagenesis and leukemogenesis. Validation of driver mutations responsible for B cell neoplasms is complicated by the volume of mutations worthy of investigation and ...
Joanna C. Dawes +3 more
doaj +1 more source
A comprehensive functional map of the hepatitis C virus genome provides a resource for probing viral proteins. [PDF]
, 2014 UnlabelledPairing high-throughput sequencing technologies with high-throughput mutagenesis enables genome-wide investigations of pathogenic organisms. Knowledge of the specific functions of protein domains encoded by the genome of the hepatitis C virus ...
Arumugaswami, Vaithilingaraja +14 more
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Mutagenesis by retroviral insertion in chemical mutagen-generated quasi-haploid mammalian cells
BioTechniques, 2007 Diploidy is a major obstacle to the mutagenic analysis of function in cultured mammalian cells. Here, we show that 6–8 rounds of chemical mutagenesis generates quasi-haploid cells that can be used as targets for insertional mutagenesis using a specially ...
Sung O. Kim +5 more
doaj +1 more source
Assessment of RNAi-induced silencing in banana (Musa spp.) [PDF]
, 2015 In plants, RNA- based gene silencing mediated by small RNAs functions at the transcriptional or post-transcriptional level to negatively regulate target genes, repetitive sequences, viral RNAs and/or transposon elements.
Cammue, B.P.A. +6 more
core +1 more source
Transcription activator like effector (TALE)-directed piggyBac transposition in human cells. [PDF]
, 2013 Insertional therapies have shown great potential for combating genetic disease and safer methods would undoubtedly broaden the variety of possible illness that can be treated. A major challenge that remains is reducing the risk of insertional mutagenesis
Bhakta, Mital S +6 more
core +3 more sources
A latent ability to persist: differentiation in Toxoplasma gondii [PDF]
, 2018 A critical factor in the transmission and pathogenesis of Toxoplasma gondii is the ability to convert from an acute disease-causing, proliferative stage (tachyzoite), to a chronic, dormant stage (bradyzoite).
Jeffers, Victoria +3 more
core +1 more source
Efficient Transposition of the Retrotransposon Tnt1 in Cucumber (Cucumis sativus L.)
Horticultural Plant Journal, 2018 Tnt1 is an active retrotransposon originally identified in tobacco (Nicotiana tabacum L.) (Grandbastien et al., 1989), but its transposition activity could be activated through tissue culture in other plant species.
Qi ZHANG +9 more
doaj +1 more source
Broad Purpose Vector for Site-Directed Insertional Mutagenesis in Bifidobacterium breve
Frontiers in Microbiology, 2021 Members of the genus Bifidobacterium are notoriously recalcitrant to genetic manipulation due to their extensive and variable repertoire of Restriction-Modification (R-M) systems.
Emily C. Hoedt +14 more
doaj +1 more source
The proppin Bcas3 and its interactor KinkyA localize to the early phagophore and regulate autophagy [PDF]
, 2021 To resolve the signaling mechanisms that mediate the starvation-induced processes of Dictyostelium sporulation and encystation, we performed insertional mutagenesis on cells harboring an mRFP-tagged spore gene.
Schaap, Pauline, Yamada, Yoko
core +2 more sources
Screening of Arabidopsis mutants for functional genomic studies [PDF]
, 2008 Eight photosynthetic Arabidopsis mutants were screened for co-segregation of a photosynthetic phenotype with the T-DNA insertion. These mutants were selected from 80 photosynthetic mutants with genetic background of Columbia-0.
Ahmad, Rashid +6 more
core +1 more source