Efficient transposon mutagenesis mediated by an IPTG-controlled conditional suicide plasmid
BMC Microbiology, 2018 Background Transposon mutagenesis is highly valuable for bacterial genetic and genomic studies. The transposons are usually delivered into host cells through conjugation or electroporation of a suicide plasmid.
Santa S. Naorem +8 more
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Development of mPing‐based activation tags for crop insertional mutagenesis
Plant Direct, 2021 Modern plant breeding increasingly relies on genomic information to guide crop improvement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits.
Alexander Johnson +12 more
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Integrase-deficient lentiviral vectors mediate efficient gene transfer to human vascular smooth muscle cells with minimal genotoxic risk [PDF]
, 2012 We have previously shown that injury-induced neointima formation was rescued by adenoviral-Nogo-B gene delivery. Integrase-competent lentiviral vectors (ICLV) are efficient at gene delivery to vascular cells but present a risk of insertional mutagenesis.
Adrian J. Thrasher +11 more
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BRE/BRCC45 regulates CDC25A stability by recruiting USP7 in response to DNA damage
Nature Communications, 2018 Loss of BRCA2 leads to cancer formation. Here, the authors use an insertional mutagenesis approach and identify a multiprotein complex consisting of BRE, USP7 and CDC25A that can support the survival of BRCA2-deficient cells.
Kajal Biswas +9 more
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Magnaporthe grisea Pathogenicity Genes Obtained Through Insertional Mutagenesis
Molecular Plant-Microbe Interactions, 1998 We have initiated a mutational analysis of pathogenicity in the rice blast fungus, Magnaporthe grisea, in which hygromycin-resistant transformants, most generated by restriction enzyme-mediated integration (REMI), were screened for the ability to infect ...
James A. Sweigard +4 more
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Development of an improved Pseudoalteromonas haloplanktis TAC125 strain for recombinant protein secretion at low temperature [PDF]
, 2008 Background: In a previous paper, we reported the accomplishment of a cold gene-expression system for the recombinant secretion of heterologous proteins in Pseudoalteromonas haloplanktis TAC125.
CIRULLI C. +3 more
core +1 more source
Generation of an Enhancer-Trapping Vector for Insertional Mutagenesis in Zebrafish. [PDF]
PLoS ONE, 2015 Enhancer trapping (ET) is a powerful approach to establish tissue- or cell-specific reporters and identify expression patterns of uncharacterized genes. Although a number of enhancer-trapping vectors have been developed and a large library of fish lines ...
Chunyan Liu +5 more
doaj +1 more source
Chromosomal DNA deletion confers phage resistance to Pseudomonas aeruginosa. [PDF]
, 2014 Bacteria develop a broad range of phage resistance mechanisms, such as prevention of phage adsorption and CRISPR/Cas system, to survive phage predation.
He, Xuesong +13 more
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Molecular Plant-Microbe Interactions, 2000 We obtained three Magnaporthe grisea morphological mutants that had the LINE transposon MGL inserted into the ACR1 locus. Sequence analysis revealed that ACR1 is homologous to medA, a developmental regulator of Aspergillus nidulans conidiation.
Marie Nishimura +5 more
doaj +1 more source
EMBO Molecular Medicine, 2016 Chimeric antigen receptor (CAR) T‐cell therapy is a new successful treatment for refractory B‐cell leukemia. Successful therapeutic outcome depends on long‐term expression of CAR transgene in T cells, which is achieved by delivering transgene using ...
Chuan Jin +5 more
doaj +1 more source