Results 41 to 50 of about 45,003 (206)
Enhanced gene trapping in mouse embryonic stem cells [PDF]
, 2008 Gene trapping is used to introduce insertional mutations into genes of mouse embryonic stem cells (ESCs). It is performed with gene trap vectors that simultaneously mutate and report the expression of the endogenous gene at the site of insertion and ...
Schnütgen, Frank +8 more
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AAV and Insertional Mutagenesis
Molecular Therapy, 2007 In this issue of Molecular Therapy, David Russell provides an invited commentary on data presented at the tenth annual meeting of the American Society of Gene Therapy that strongly suggested that adenovirus-associated virus (AAV) vector insertion could in certain models be linked to the development of cancer.1 A short report describing the data that ...
openaire +2 more sources
L1 Retrotransposon Heterogeneity in Ovarian Tumor Cell Evolution
Cell Reports, 2018 Summary: LINE-1 (L1) retrotransposons are a source of insertional mutagenesis in tumor cells. However, the clinical significance of L1 mobilization during tumorigenesis remains unclear.
Thu H.M. Nguyen +22 more
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Detecting statistically significant common insertion sites in retroviral insertional mutagenesis screens. [PDF]
PLoS Computational Biology, 2006 Retroviral insertional mutagenesis screens, which identify genes involved in tumor development in mice, have yielded a substantial number of retroviral integration sites, and this number is expected to grow substantially due to the introduction of high ...
Jeroen de Ridder +4 more
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Molecular Therapy: Methods & Clinical Development, 2017 Wiskott-Aldrich syndrome (WAS) is a life-threatening immunodeficiency caused by mutations within the WAS gene. Viral gene therapy to restore WAS protein (WASp) expression in hematopoietic cells of patients with WAS has the potential to improve outcomes ...
Swati Singh +11 more
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Germline transgenesis and insertional mutagenesis in the ascidian Ciona intestinalis [PDF]
, 2007 Stable transgenesis is a splendid technique that is applicable to the creation of useful marker lines,enhancer/gene traps, and insertional mutagenesis.
Sasakura Yasunori, 笹倉 靖徳
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Cancer Gene Discovery: Exploiting Insertional Mutagenesis [PDF]
Molecular Cancer Research, 2013 Abstract Insertional mutagenesis has been used as a functional forward genetics screen for the identification of novel genes involved in the pathogenesis of human cancers. Different insertional mutagens have been successfully used to reveal new cancer genes.
Marco, Ranzani +3 more
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Genome-wide transposon mutagenesis of paramyxoviruses reveals constraints on genomic plasticity.
PLoS Pathogens, 2020 The antigenic and genomic stability of paramyxoviruses remains a mystery. Here, we evaluate the genetic plasticity of Sendai virus (SeV) and mumps virus (MuV), sialic acid-using paramyxoviruses that infect mammals from two Paramyxoviridae subfamilies ...
Satoshi Ikegame +8 more
doaj +1 more source
ApuA, a multifunctional x-glucan-degrading enzyme of Streptococcus suis, mediates adhesion to porcine epithelium and mucus [PDF]
, 2010 We have identified apuA in Streptococcus suis, which encodes a bifunctional amylopullulanase with conserved -amylase and pullulanase substrate-binding domains and catalytic motifs.
Ferrando, M.L. +4 more
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Random Insertional Mutagenesis of a Serotype 2 Dengue Virus Clone
Bio-Protocol, 2018 Protein tagging is a powerful method of investigating protein function. However, modifying positive-strand RNA virus proteins in the context of viral infection can be particularly difficult as their compact genomes and multifunctional proteins mean even ...
Jeffrey Perry, Andrew Tai
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