FSTVAL: a new web tool to validate bulk flanking sequence tags
Plant Methods, 2012 Background Information about a transgene locus is one of the major concerns in transgenic research because expression of the transgene or a gene interrupted by the integration event could be affected. Thus, the flanking sequences obtained from transgenic
Kim Joung +11 more
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Light controls growth and development via a conserved pathway in the fungal kingdom. [PDF]
PLoS Biology, 2005 Light inhibits mating and haploid fruiting of the human fungal pathogen Cryptococcus neoformans, but the mechanisms involved were unknown. Two genes controlling light responses were discovered through candidate gene and insertional mutagenesis approaches.
Alexander Idnurm, Joseph Heitman
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, 2016 Background: Knowledge of the intrinsic properties of embryonic stem cells is essential before the possibility of using these cells for therapeutic purposes becomes a reality. Insertional mutagenesis screens are widely used to identify genes sufficient to
Lee, Sung-Hun, Rangasamy, Danny
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The expanding universe of transposon technologies for gene and cell engineering [PDF]
, 2010 Transposable elements can be viewed as natural DNA transfer vehicles that, similar to integrating viruses, are capable of efficient genomic insertion.
Zoltán Ivics, Zsuzsanna Izsvák
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Conditional ablation of the RFX4 isoform 1 transcription factor: Allele dosage effects on brain phenotype. [PDF]
PLoS ONE, 2018 Regulatory factor X4 (RFX4) isoform 1 is a recently discovered isoform of the winged helix transcription factor RFX4, which can bind to X-box consensus sequences that are enriched in the promoters of cilia-related genes.
Ping Xu +6 more
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Respiratory-deficient mutants of the unicellular green alga Chlamydomonas: A review. [PDF]
, 2013 Genetic manipulation of the unicellular green alga Chlamydomonas reinhardtii is straightforward. Nuclear genes can be interrupted by insertional mutagenesis or targeted by RNA interference whereas random or site-directed mutagenesis allows the ...
Cardol, Pierre +4 more
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Transducin beta-like gene FTL1 is essential for pathogenesis in Fusarium graminearum [PDF]
, 2009 Fusarium head blight caused by Fusarium graminearum is an important disease of wheat and barley. In a previous study, we identified several mutants with reduced virulence by insertional mutagenesis. A transducin beta-like gene named FTL1 was disrupted in
Ding, S.L. +7 more
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A Graph Based Framework to Model Virus Integration Sites
Computational and Structural Biotechnology Journal, 2016 With next generation sequencing thousands of virus and viral vector integration genome targets are now under investigation to uncover specific integration preferences and to define clusters of integration, termed common integration sites (CIS), that may ...
Raffaele Fronza +3 more
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Early stages of leaf development in has mutant of Arabidopsis thaliana
Biologia Plantarum, 2011 The elucidation of molecular mechanisms underlying the leaf development can be facilitated by the detailed anatomical study of leaf development mutants.
D. Janošević +4 more
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Efficient Gene Targeting Mediated by Adeno-Associated Virus and DNA Double-Strand Breaks [PDF]
, 2003 Gene targeting is the in situ manipulation of the sequence of an endogenous gene by the introduction of homologous exogenous DNA. Presently, the rate of gene targeting is too low for it to be broadly used in mammalian somatic cell genetics or to cure ...
Baltimore, David +3 more
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