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In Vitro Site Directed Mutagenesis
2023Site-Directed Mutagenesis (SDM) allows for changes in the DNA sequence of plasmids using polymerase chain reaction (PCR). It is a reliable, accessible, and rapid method which is the common initial step of many biochemial or genetic experiments. Here we describe the various different forms of SDM before giving a detailed method for the introduction of ...
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Trends in Biochemical Sciences, 1982
The development of genetic engineering, whereby a specific gene or cDNA (c is copy or complementary) can be isolated as part of a minichromosome that can replicate and be expressed (by transcription and translation) in a living cell, has made possible in vitro techniques for micromanipulation (i.e. site-directed mutagenesis) of a cloned gene, to make
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The development of genetic engineering, whereby a specific gene or cDNA (c is copy or complementary) can be isolated as part of a minichromosome that can replicate and be expressed (by transcription and translation) in a living cell, has made possible in vitro techniques for micromanipulation (i.e. site-directed mutagenesis) of a cloned gene, to make
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Site-Directed Mutagenesis of Bifidobacterium Strains
2021At present, only a limited number of Bifidobacterium species are amenable to genetic manipulation using mutagenesis. This lack of genetic accessibility among the majority of bifidobacterial strains represents a significant roadblock for the study of gene function and expression in these potential probiotics.
Kieran, James, Douwe, van Sinderen
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Inverse PCR for Site-Directed Mutagenesis
2023Inverse PCR is a powerful tool for the rapid introduction of desired mutations at desired positions in a circular double-stranded DNA sequence. In this technique, custom-designed mutant primers oriented in the inverse direction are used to amplify the entire circular template with incorporation of the required mutation(s).
Diogo, Silva +4 more
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Trends in Neurosciences, 1984
Much of what neurons do they do with proteins. From functional studies we learn how these proteins operate, and from chemical and molecular biological research we learn about their molecular structures. But how are studies of structure and function to be connected?
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Much of what neurons do they do with proteins. From functional studies we learn how these proteins operate, and from chemical and molecular biological research we learn about their molecular structures. But how are studies of structure and function to be connected?
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High-Throughput Site-Directed Mutagenesis
2019Protein engineering has an array of uses: whether you are studying a disease mutation, removing undesirable sequences, adding stabilizing mutations for structural purposes, or simply dissecting protein function. Protein engineering is almost exclusively performed using site-directed mutagenesis (SDM) as this provides targeted modification of specific ...
Claire, Strain-Damerell +1 more
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[14] Site-directed mutagenesis
1995Publisher Summary This chapter discusses the design and implementation of site-directed mutagenesis approaches to elucidating the structure–function relationships of proteins. Before the development of recombinant deoxyribonucleic acid (DNA) technology, the structure–function relationships of proteins were explored by chemical modification of the ...
Tung Ming Fong +2 more
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Massive Mutagenesis®: High-Throughput Combinatorial Site-Directed Mutagenesis
2010Massive Mutagenesis is a proprietary library creation method that enables the fast generation of high-quality genetic libraries. Starting from a single gene on a plasmid and hundreds to thousands of oligonucleotides, a one-step single-strand circular amplification method creates random combinations of several site-directed substitutions, insertions, or
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