Results 211 to 220 of about 349,101 (299)

Zebrafish and CRISPR—A synergistic approach to decipher and cure human diseases

Animal Models and Experimental Medicine, EarlyView.
Zebrafish, with high genetic homology to humans, serves as a powerful vertebrate model for disease modeling and drug discovery. Integration of CRISPR/Cas9 technology enables precise genome editing, facilitating the development of translational models for human diseases.
Manikandan Sivaprakasam, Aashika Raagavi Jeanpierre, Salma Mohammed, Rajesh Srinivasan, Aman Kumar Mohanty   +4 more
wiley   +1 more source

Systematic mining and engineering of signal peptides to achieve efficient secretion of cephalosporin C acylase in <i>Escherichia coli</i>. [PDF]

Synth Syst Biotechnol
Ren X, Yang Z, Chen S, Pu C, Wang H, Li J, Cao Q, Huang X, Fu G, Yuan B, Luo J, Sun Z, Dong H, Zhang D.   +13 more
europepmc   +1 more source

Promiscuity‐Guided Enzyme Evolution via Substrate Multiplexed Screening

Angewandte Chemie, EarlyView.
Through historical context and recent examples, this Minireview summarizes the strengths, limitations, and practical considerations for the implementation of substrate multiplexed screening (SUMS) in enzyme evolution. ABSTRACT An enzyme's ability to react with diverse substrates while maintaining high yield and selectivity, termed “substrate ...
Holly A. Weilbaker, Meghan E. Campbell, Peyton M. Higgins, Andrew R. Buller   +3 more
wiley   +2 more sources

A Single Flavoenzyme Forges the Pentacyclic Skeleton of α-Cyclopiazonic Acid. [PDF]

J Am Chem Soc
Wang M, Cheng T, Zhang W, Kuo YM, Li Y, Yan Y, Hisao PY, Chang CY, Wang N.   +8 more
europepmc   +1 more source

<i>Crithidia fasciculata</i> gene of unknown function CFAC1_070014400 encodes a putative alpha/beta hydrolase with a conserved active site. [PDF]

MicroPubl Biol
Davis J.
europepmc   +1 more source

Use of gain-of-function suppressor screening to elucidate <i>Candida albicans</i> Cdr1 efflux mechanism, inhibition, and drug resistance. [PDF]

Front Microbiol
Zhao M, Niimi M, Cannon RD, Lamping E.
europepmc   +1 more source

Mutation of leucine 170 alters the subcellular distribution, neurite outgrowth and three-dimensional structure of dystrophins Dp71 and Dp40. [PDF]

Mol Biol Rep
García-Cruz C, Sanchez-Perez L, Herrera-Rojas F, Posadas-Trejo M, Ceja V, Sánchez-Trujillo A, Merino-Jiménez C, Aragón J, Montanez C.   +8 more
europepmc   +1 more source

Ribozymes for RNA‐Catalyzed RNA Methylation and Labeling

Angewandte Chemie International Edition, EarlyView.
Ribozymes are powerful tools for site‐specific RNA modification. Their activities range from installing tags and fluorophores to generating natural RNA methylations, making them valuable tools to uncover the many functions that RNA plays in nature.
Carolin P. M. Scheitl, Claudia Höbartner   +1 more
wiley   +1 more source

P450 engineering via structure-guided rational design achieves high C21-selectivity and bioconversion in steroid biosynthesis. [PDF]

Synth Syst Biotechnol
Yang J, Li R, Gao Q, Ma L, Wang Q, Ma G, Fan Y, Li S, Xu LH.   +8 more
europepmc   +1 more source