Results 81 to 90 of about 349,101 (299)

Creating Randomized Amino Acid Libraries with the QuikChange® Multi Site-Directed Mutagenesis Kit

BioTechniques, 2002
The QuikChange® Multi Site-Directed Mutagenesis Kit is a simple and efficient method for introducing point mutations at up to five sites simultaneously in plasmid DNA templates. Here we used the QuikChange Multi kit with degenerate (one codon) primers to
Holly H. Hogrefe, Janice Cline, Geri L. Youngblood, Ronda M. Allen   +3 more
doaj   +1 more source

Converting CooA from a Carbon Monoxide to an Oxygen-Sensing Heme Protein Transcription Factor: Investigations into the Structure and Mechanism of Gas Binding [PDF]

, 2011
CooA is a carbon monoxide-sensing (CO-sensing) heme protein transcription factor that regulates gene activation in several bacteria and, importantly, is a convenient model for studying analogous proteins in the human body.
Sedoris, Amanda, Wagoner, Josh
core   +2 more sources

Structural and functional determination of homologs of the Mycobacterium tuberculosis N-acetylglucosamine-6-phosphate deacetylase (NagA) [PDF]

, 2018
The (Mtb) pathogen encodes an -acetylglucosamine-6-phosphate deacetylase enzyme, NagA (Rv3332), that belongs to the amidohydrolase superfamily. NagA enzymes catalyze the deacetylation of -acetylglucosamine-6-phosphate (GlcNAc6P) to glucosamine-6 ...
Ahangar, Mohd Syed, Cameron, Alexander, Cooper, Charlotte, Fullam, Elizabeth, Furze, Christopher M., Graham, Ben, Guy, Collette S., Maskew, Kathryn S   +7 more
core   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Effects of mutations in the helix G region of horseradish peroxidase [PDF]

, 2008
Horseradish peroxidase (HRP) has long attracted intense research interest and is used in many biotechnological fields, including diagnostics, biosensors and biocatalysis.
Barry J. Ryan, Batra, Chakrabarti, Chang, Chattopadhyay, Ciarán Ó'Fágáin, Coates, Dai, Dunford, French, Gajhede, Garcia, Gonera, Gorman, Grigorenko, Guex, Hakamada, Haque, Hassani, Howes, Ignatenko, Jager, Kaposi, Khmelnitsky, Krowarsch, Kwon, Lei, Lin, Lipovsek, Liu, Liu, Machado, Miland, Miland, Mogharrab, Moosavi-Movahedi, Morawski, Nagano, Numata, O'Brien, O'Brien, Orlova, Ozaki, Pina, Priori, Ryan, Ryan, Ryan, Ryu, Sambrook, Santucci, Savenkova, Smeller, Smith, Tams, Tanaka, Tanaka, Ugarova, Urrutigoity, Wang   +59 more
core   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Extensive site-directed mutagenesis reveals interconnected functional units in the alkaline phosphatase active site

eLife, 2015
Enzymes enable life by accelerating reaction rates to biological timescales. Conventional studies have focused on identifying the residues that have a direct involvement in an enzymatic reaction, but these so-called ‘catalytic residues’ are embedded in ...
Fanny Sunden, Ariana Peck, Julia Salzman, Susanne Ressl, Daniel Herschlag   +4 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Epilepsy‐Associated Variants of a Single SCN1A Codon Exhibit Divergent Functional Properties

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Pathogenic variants in SCN1A, which encodes the voltage‐gated sodium channel NaV1.1, are associated with multiple epilepsy syndromes exhibiting a range of clinical severity. SCN1A variants are reported in different syndromes, including Dravet syndrome, which is associated with loss‐of‐function, whereas neonatal/infantile‐onset ...
Lanie N. Liebovitz, Christopher H. Thompson, Linda C. Laux, Alfred L. George Jr.   +3 more
wiley   +1 more source

Multiple-site fragment deletion, insertion and substitution mutagenesis by modified overlap extension PCR

Biotechnology & Biotechnological Equipment, 2017
Introducing various mutations at multiple specific sites within a gene requires multiple steps of DNA manipulation, which is the initial, but limiting step of protein structure–function studies.
Fanli Zeng, Yujie Zhang, Ze Zhang, Asrar Ahmad Malik, Yibin Lin   +4 more
doaj   +1 more source