Results 61 to 70 of about 247,235 (200)
Primers used for random and site-directed mutagenesis. [PDF]
, 2013 a, random mutagenesis; b, site-directed mutagenesis. Underline letters indicate the sequence for ligation-independent cloning; Italic letters indicate the mutation nucleotides.
Hanbin Liu (370302) +11 more
core +1 more source
eLife, 2015 Enzymes enable life by accelerating reaction rates to biological timescales. Conventional studies have focused on identifying the residues that have a direct involvement in an enzymatic reaction, but these so-called ‘catalytic residues’ are embedded in ...
Fanny Sunden +4 more
doaj +1 more source
Construction of a high-efficiency multi-site-directed mutagenesis [PDF]
, 2013 Although site-directed mutagenesis has been used in many fields, it still has low rate of success and high cost because of low-yield target products. A modified method for multi-site-directed mutagenesis was developed with shifted primer design and cold ...
Chen, L +4 more
core +2 more sources
Primers used for site-directed mutagenesis. [PDF]
, 2014 The asterisk (*) indicate position of deleted nucleotide in forward primer. Analogous deletion was introduced in the reverse primer but is not shown for clarity of presentation.Primers used for site-directed mutagenesis.
Sylwia Bloch (643380) +6 more
core +1 more source
Biotechnology & Biotechnological Equipment, 2017 Introducing various mutations at multiple specific sites within a gene requires multiple steps of DNA manipulation, which is the initial, but limiting step of protein structure–function studies.
Fanli Zeng +4 more
doaj +1 more source
Oligonucleotides used for site-directed mutagenesis (forward primer). [PDF]
, 2016 Oligonucleotides used for site-directed mutagenesis (forward primer).
Xiuju Li (700858) +3 more
core +1 more source
Antimicrobial characterization of site-directed mutagenesis of porcine beta defensin 2.
PLoS ONE, 2015 Porcine β defensin 2 (pBD2) is a small, cationic and amphiphilic antimicrobial peptide. It has broad antimicrobial activities against bacteria and plays an important role in host defense.
Xian-xian Huang +3 more
doaj +1 more source
Scarless and site-directed mutagenesis in
BMC Biotechnology, 2007 Background A variety of techniques have been described which introduce scarless, site-specific chromosomal mutations. These techniques can be applied to make point mutations or gene deletions as well as insert heterologous DNA into bacterial vectors for ...
Berghman Luc R +7 more
doaj +1 more source
List of oligonucleotides used for site-directed mutagenesis. [PDF]
, 2013 List of oligonucleotides used for site-directed mutagenesis.
Stefan Knapp (244328) +6 more
core +1 more source
Primers for PCR, sequencing, and site-directed mutagenesis. [PDF]
, 2013 Primers for PCR, sequencing, and site-directed mutagenesis.
James M. Mason (103110) +3 more
core +1 more source