Results 51 to 60 of about 349,101 (299)

The Primer Generator: A Program that Facilitates the Selection of Oligonucleotides for Site-Directed Mutagenesis

BioTechniques, 1999
Site-directed mutagenesis is a powerful tool that has enabled molecular biologists to perform functional analysis of altered nucleic acids and proteins.
Alexander Turchin, Joseph F. Lawler
doaj   +1 more source

Hippo pathway at the crossroads of stemness and therapeutic resistance in breast cancer

Molecular Oncology, EarlyView.
Dysregulation of the Hippo pathway drives nuclear accumulation of YAP/TAZ, activating stemness‐related transcriptional programs that sustain breast cancer stemness and fuel therapeutic resistance across subtypes, underscoring Hippo signaling as a targetable vulnerability. Figure created and edited with BioRender.com.
Giulia Schiavoni, Antonella Palmese, Stefano Scalera, Laura Cipriani, Davide Mascolo, Patrizia Vici, Teresa Arcuri, Lorena Filomeno, Eriseld Krasniqi, Giovanni Blandino, Giulia Bon, Marcello Maugeri‐Saccà   +11 more
wiley   +1 more source

Identification of a Key Amino Acid of the Human 5-HT2B Serotonin Receptor Important for Sarpogrelate Binding

Journal of Pharmacological Sciences, 2007
Based on radio-ligand binding and molecular modeling studies, sarpogrelate shows a moderate selectivity for 5-HT2B versus 5-HT2A receptors. To confirm the modeling data of sarpogrelate to 5-HT2B receptors predicting interaction of sarpogrelate towards ...
Habib Abul Muntasir, Murad Hossain, Mohiuddin Ahmed Bhuiyan, Tadazumi Komiyama, Takashi Nakamura, Masanobu Ozaki, Takafumi Nagatomo   +6 more
doaj   +1 more source

Broad Purpose Vector for Site-Directed Insertional Mutagenesis in Bifidobacterium breve

Frontiers in Microbiology, 2021
Members of the genus Bifidobacterium are notoriously recalcitrant to genetic manipulation due to their extensive and variable repertoire of Restriction-Modification (R-M) systems.
Emily C. Hoedt, Emily C. Hoedt, Francesca Bottacini, Francesca Bottacini, Nora Cash, Roger S. Bongers, Kees van Limpt, Kaouther Ben Amor, Jan Knol, Jan Knol, John MacSharry, John MacSharry, John MacSharry, Douwe van Sinderen, Douwe van Sinderen   +14 more
doaj   +1 more source

Site directed mutagenesis of L-pyrrolysine in dimethylamine methyltransferase [PDF]

, 2009
Methanosarcina barkeri is an archaeon and is able to use methylamines as growth substrates for methanogenesis. The methylamine methyltransferases that are involved in initiating methanogenesis from the methylamines have an in-frame amber codon (UAG) that
Patel, Jigar
core  

Horseradish and soybean peroxidases: comparable tools for alternative niches? [PDF]

, 2006
Horseradish and soybean peroxidases (HRP and SBP, respectively) are useful biotechnological tools. HRP is often termed the classical plant heme peroxidase and although it has been studied for decades, our understanding has deepened since its cloning and ...
Alonso Lomillo, Alpeeva, Azevedo, Azevedo, Azevedo, Barry J. Ryan, Bruns, Bódalo, Campanella, Castilho, Castillo, Chen, Cho, Ciarán Ó’Fágáin, Colas, Dachs, Dai, Dai, dos Santos, Dotsikas, Dunford, Duroux, Ferapontova, Ferapontova, Ferapontova, Folkes, Gajhede, Gazaryan, Gazaryan, Gijzen, Gray, Gustafsson, Henriksen, Hoffmann, Howes, Howes, Howes, Hoyle, Hushpulian, Ignatenko, Jung, Kamal, Kamiya, Katz, Kazlauskas, Kenausis, Kim, Kim, Klibanov, Knutson, Konash, Kreiner, Lee, Lei, Li, Li, Lin, Lin, Lindgren, Liu, Luong, Mendive, Michizoe, Morawski, Morawski, Morgan, Morimoto, Mukai, Nagano, Neil Carolan, Newmyer, Newmyer, Nicell, Ozaki, Patel, Paul, Penalva, Poljaková, Rahman, Rauf, Rodriguez-Lopez, Rossiter, Sakharov, Schmitz, Schuvailo, Shipovskov, Smith, Smith, Song, Sun, Tafazoli, Tanaka, Teilum, Tupper, Tønning, van de Velde, van de Velde, Varnado, Veitch, Veitch, Veitch, Veitch, Verpoorte, Vreeke, Wandersman, Wang, Wang, Wanger, Welinder, Welinder, Welinder, Won, Woodard, Wu, Wuhrer, Yan, Yuan, Zhang, Zhang, Zhu   +119 more
core   +2 more sources

Hijacking emergency granulopoiesis: Neutrophil ontogeny and reprogramming in cancer

Molecular Oncology, EarlyView.
Neutrophils are highly plastic innate immune cells; their functions in cancer extend beyond the tumour microenvironment. This Review summarises current understanding of neutrophil maturation and heterogeneity and highlights tumour‐induced granulopoiesis as a systemic programme that expands immature, immunosuppressive neutrophils via tumour‐derived ...
Gabriela Marinescu, Yi Feng
wiley   +1 more source

Human carboxylesterase 2: Studies on the role of glycosylation for enzymatic activity

Biochemistry and Biophysics Reports, 2016
Human carboxylesterase 2 (hCES2) is a glycoprotein involved in the metabolism of drugs and several environmental xenobiotics, whose crystallization has been proved to be a challenging task.
Márcia Alves, Joana Lamego, Tiago Bandeiras, Rute Castro, Hélio Tomás, Ana Sofia Coroadinha, Júlia Costa, Ana Luisa Simplício   +7 more
doaj   +1 more source

High-throughput Site-directed Scanning Mutagenesis Using a Two-fragment PCR Approach

Bio-Protocol, 2020
Site-directed scanning mutagenesis is a useful tool applied in studying protein function and designing proteins with new properties, such as increased stability or enzymatic activity. Creating a systematic library of hundreds of site-directed mutants is
Franziska Heydenreich, Tamara Miljuš, Dalibor Milić, Dmitry Veprintsev   +3 more
doaj   +1 more source

The structure of HPr and site-directed mutagenesis [PDF]

FEMS Microbiology Letters, 1989
Keywords: Phosphoryl transfer; Epitopes; Two-dimensional NMR; Protein structure; X-ray diffraction; Sugar ...
E B, Waygood, S, Sharma, P, Bhanot, O A, el-Kabbani, L T, Delbaere, F, Georges, M G, Wittekind, R E, Klevit   +7 more
openaire   +2 more sources