Results 41 to 50 of about 247,235 (200)

Broad Purpose Vector for Site-Directed Insertional Mutagenesis in Bifidobacterium breve

Frontiers in Microbiology, 2021
Members of the genus Bifidobacterium are notoriously recalcitrant to genetic manipulation due to their extensive and variable repertoire of Restriction-Modification (R-M) systems.
Emily C. Hoedt, Emily C. Hoedt, Francesca Bottacini, Francesca Bottacini, Nora Cash, Roger S. Bongers, Kees van Limpt, Kaouther Ben Amor, Jan Knol, Jan Knol, John MacSharry, John MacSharry, John MacSharry, Douwe van Sinderen, Douwe van Sinderen   +14 more
doaj   +1 more source

Identification of amino acid residues in the ligand binding repeats of LDL receptor important for PCSK9 binding[S]

Journal of Lipid Research, 2019
Proprotein convertase subtilisin/kexin type 9 (PCSK9) promotes LDL receptor (LDLR) degradation, increasing plasma levels of LDL cholesterol and the risk of cardiovascular disease.
Shi-jun Deng, Adekunle Alabi, Hong-mei Gu, Ayinuer Adijiang, Shucun Qin, Da-wei Zhang   +5 more
doaj   +1 more source

The Primer Generator: A Program that Facilitates the Selection of Oligonucleotides for Site-Directed Mutagenesis

BioTechniques, 1999
Site-directed mutagenesis is a powerful tool that has enabled molecular biologists to perform functional analysis of altered nucleic acids and proteins.
Alexander Turchin, Joseph F. Lawler
doaj   +1 more source

Oligonucleotide primers used for site-directed mutagenesis. [PDF]

, 2017
Oligonucleotide primers used for site-directed mutagenesis.
Sheng Chen (21971), Xuguo Duan (1888171), Jing Wu (54032), Zhu Li (193162)   +3 more
core   +1 more source

Study on increase of nicotinamide mononucleotide yield by site-directed mutagenesis of nicotinamide phosphoribosyltransferase [PDF]

Zhongguo niangzao
Nicotinamide phosphoribosyltransferase (NAMPT) was a key rate-limiting enzyme in the biosynthetic pathway of nicotinamide mononucleotide (NMN). In this study, the NAMPT gene derived from Comamonadaceae bacterium was heterologous expressed in Escherichia ...
DONG Ping, XU Yifan, HOU Ying, LUO Xuegang, ZHANG Lin, QIAO Changsheng
doaj   +1 more source

High-throughput Site-directed Scanning Mutagenesis Using a Two-fragment PCR Approach

Bio-Protocol, 2020
Site-directed scanning mutagenesis is a useful tool applied in studying protein function and designing proteins with new properties, such as increased stability or enzymatic activity. Creating a systematic library of hundreds of site-directed mutants is
Franziska Heydenreich, Tamara Miljuš, Dalibor Milić, Dmitry Veprintsev   +3 more
doaj   +1 more source

Parameter prediction of site-directed mutagenesis. [PDF]

, 2015
Parameter prediction of site-directed mutagenesis.
Chun-yu Gao (696324), Chun-li Li (696326), Xian-xian Huang (696323), Qing-jun Zhao (696325)   +3 more
core   +1 more source

Human carboxylesterase 2: Studies on the role of glycosylation for enzymatic activity

Biochemistry and Biophysics Reports, 2016
Human carboxylesterase 2 (hCES2) is a glycoprotein involved in the metabolism of drugs and several environmental xenobiotics, whose crystallization has been proved to be a challenging task.
Márcia Alves, Joana Lamego, Tiago Bandeiras, Rute Castro, Hélio Tomás, Ana Sofia Coroadinha, Júlia Costa, Ana Luisa Simplício   +7 more
doaj   +1 more source

Multiple site-directed mutagenesis via simple cloning by prolonged overlap extension

BioTechniques, 2020
We describe the application of simple cloning by prolonged overlap extension for multiple site-directed mutagenesis in the same plasmid. We show that it is possible to use this technique with very short PCR templates.
Rasmus Hejlesen, Ernst-Martin Füchtbauer   +1 more
doaj   +1 more source

Site Directed Mutagenesis of YDL124w [PDF]

, 2014
Site Directed Mutagenesis of YDL124w. The purpose of this research project is to better understand the catalytic activity of the Saccharomyces cerevisiae enzyme ...
Owens, Zachary, Smith, Jeremy
core