Results 41 to 50 of about 3,838,500 (295)
, 2012 In vivo site-directed mutagenesis by ssDNA recombineering is a facile method to change the color of fluorescent proteins without cloning. Two different starting alleles of GFP were targeted for mutagenesis: gfpmut3* residing in the E.
Ceballos, Melvys Valledor +7 more
core +1 more source
Human carboxylesterase 2: Studies on the role of glycosylation for enzymatic activity
Biochemistry and Biophysics Reports, 2016 Human carboxylesterase 2 (hCES2) is a glycoprotein involved in the metabolism of drugs and several environmental xenobiotics, whose crystallization has been proved to be a challenging task.
Márcia Alves +7 more
doaj +1 more source
The structure of HPr and site-directed mutagenesis [PDF]
FEMS Microbiology Letters, 1989 Keywords: Phosphoryl transfer; Epitopes; Two-dimensional NMR; Protein structure; X-ray diffraction; Sugar ...
E B, Waygood +7 more
openaire +2 more sources
Finding novel vulnerabilities of hypomorphic BRCA1 alleles
Molecular Oncology, EarlyView.Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder +10 more
wiley +1 more source
Directed evolution of transketolase, a carbon-carbon forming enzyme. [PDF]
, 2005 The enzyme transketolase (Enzyme Commission number: 2.2.1.1) has significant potential as a biocatalyst in the production of pharmaceuticals and fine chemicals.
Miller, O.J.
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Mutagenesis sites, primers and templates for site-directed mutagenesis.
, 2013 Mutagenesis sites, primers and templates for site-directed mutagenesis.
James M. Mason (103110) +3 more
core +1 more source
Primers used for site directed mutagenesis.
, 2015 Primers used for site directed mutagenesis.
Pascual Sanz (208343) +2 more
core +3 more sources
High-throughput Site-directed Scanning Mutagenesis Using a Two-fragment PCR Approach
Bio-Protocol, 2020 Site-directed scanning mutagenesis is a useful tool applied in studying protein function and designing proteins with new properties, such as increased stability or enzymatic activity. Creating a systematic library of hundreds of site-directed mutants is
Franziska Heydenreich +3 more
doaj +1 more source
Molecular Oncology, EarlyView.Drug resistance limits treatment success in a subset of lung cancers driven by ROS1 gene alterations. Using patient‐derived cells and computer simulations, we studied three key mutations and how they affect five targeted drugs. The mutations reduced drug effectiveness in different ways by altering protein structure and behavior.
Farhan Ul Haq +8 more
wiley +1 more source
Different Approaches For Protein Engineering In Industrial Biotechnology [PDF]
, 2011 Protein engineering is the novel field which has wide applications from pharmaceutics, industry, commercial, laundry and research. It may apply rational design or non rational design or both.
Muhammad Waheed Akhtar, Israr Khan
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