Results 21 to 30 of about 247,235 (200)
BMC Biotechnology, 2004 Background Site-directed mutagenesis is an efficient method to alter the structure and function of genes. Here we report a rapid and efficient megaprimer-based polymerase chain reaction (PCR) mutagenesis strategy that by-passes any intermediate ...
Duggleby Ronald G +2 more
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Primers for site-directed mutagenesis. [PDF]
, 2014 Note: The mutated sites are underlined in bold and italic.Primers for site-directed mutagenesis.
Haihong Hao (309782) +8 more
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Journal of Lipid Research, 1998 Both apolipoprotein (apo) E2 and apoE-Leiden (tandem repeat of amino acids 121–127) are associated with type III hyperlipoproteinemia and bind defectively to low density lipoprotein receptors. Removing the carboxyl terminus of both variants (residues 192–
Li-Ming Dong +4 more
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Computationally Optimizing the Directed Evolution of Proteins [PDF]
, 2003 Directed evolution has proven a successful strategy for protein engineering. To accelerate the discovery process, we have developed several computational methods to optimize the mutant libraries by targeting specific residues for mutagenesis, and ...
Voigt, Christopher Ashby
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Primer sequences for site directed mutagenesis. [PDF]
, 2019 Primer sequences for site directed mutagenesis.
Dolunay Kelle (6388055) +3 more
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In silico Analysis and Site-directed Mutagenesis of Promoters
Bio-Protocol, 2017 In normal as in cancerous cells, gene expression is tightly regulated by transcription factors, which are responsible for up- or down-regulation of thousands of targets involved in different cell processes. Transcription factors can directly regulate the
Salah Boudjadi, Jean-François Beaulieu
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Site-directed mutation of a laccase from Thermus thermophilus: Effect on the activity profile [PDF]
Archives of Biological Sciences, 2012 A site-directed mutant R453T of a laccase from Thermus thermophilus HB27 (Tth-laccase) was constructed in order to investigate the effect on laccase catalytic properties. The mutated gene was cloned and overexpressed in Escherichia coli.
Liu Xin, Tian Meng, Liu Kewu
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Site-directed mutagenesis studies of conserved and group-specific residues in adenylate-forming enzymes. [PDF]
, 2018 Site-directed mutagenesis studies of conserved and group-specific residues in adenylate-forming enzymes.
Danielle Leatherby (5714819) +4 more
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Primer sequences for site-directed mutagenesis. [PDF]
, 2013 Primer sequences for site-directed mutagenesis.
Zhiyun Wei (165410) +7 more
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Current status and emerging frontiers in enzyme engineering: An industrial perspective
HeliyonProtein engineering mechanisms can be an efficient approach to enhance the biochemical properties of various biocatalysts. Immobilization of biocatalysts and the introduction of new-to-nature chemical reactivities are also possible through the same ...
Obinna Giles Ndochinwa +6 more
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