SDM-assist software to design site-directed mutagenesis primers introducing "silent" restriction sites [PDF]
, 2013 BACKGROUND:Over the past decades site-directed mutagenesis (SDM) has become an indispensable tool for biological structure-function studies. In principle, SDM uses modified primer pairs in a PCR reaction to introduce a mutation in a cDNA insert.
Abhijit Karnik +5 more
core +5 more sources
Linking structure and function of the asialoglycoprotein receptor H1-CRD using site-directed mutagenesis and isotope labeling [PDF]
, 2007 The asialoglycoprotein receptor (ASGP-R) is a C-type lectin, abundantly expressed on hepatocytes. It mediates the clearance of desialylated glycoproteins carrying terminal galactose (Gal) or N-acetyl-galactosamine (GalNAc) residues through endocytosis ...
Johansson, Anna Karin
core +1 more source
VH-RELATED IDIOTOPES DETECTED BY SITE-DIRECTED MUTAGENESIS [PDF]
, 1992 The function of the CD4 cell surface protein as coreceptor on T helper lymphocytes and as receptor for HIV makes this glycoprotein a prime target for an immune intervention with mAb.
Rieber, Ernst Peter +4 more
core +1 more source
Identification of a key residue for Oligomerisation and pore-formation of Clostridium perfringens NetB [PDF]
, 2014 Necrotic enteritis toxin B (NetB) is a β-pore-forming toxin produced by Clostridium perfringens and has been identified as a key virulence factor in the pathogenesis of avian necrotic enteritis, a disease causing significant economic damage to the ...
Richard W. Titball +24 more
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Journal of Lipid Research, 1998 Both apolipoprotein (apo) E2 and apoE-Leiden (tandem repeat of amino acids 121–127) are associated with type III hyperlipoproteinemia and bind defectively to low density lipoprotein receptors. Removing the carboxyl terminus of both variants (residues 192–
Li-Ming Dong +4 more
doaj +1 more source
Computationally Optimizing the Directed Evolution of Proteins [PDF]
, 2003 Directed evolution has proven a successful strategy for protein engineering. To accelerate the discovery process, we have developed several computational methods to optimize the mutant libraries by targeting specific residues for mutagenesis, and ...
Voigt, Christopher Ashby
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Site-directed mutation of a laccase from Thermus thermophilus: Effect on the activity profile [PDF]
Archives of Biological Sciences, 2012 A site-directed mutant R453T of a laccase from Thermus thermophilus HB27 (Tth-laccase) was constructed in order to investigate the effect on laccase catalytic properties. The mutated gene was cloned and overexpressed in Escherichia coli.
Liu Xin, Tian Meng, Liu Kewu
doaj +1 more source
In silico Analysis and Site-directed Mutagenesis of Promoters
Bio-Protocol, 2017 In normal as in cancerous cells, gene expression is tightly regulated by transcription factors, which are responsible for up- or down-regulation of thousands of targets involved in different cell processes. Transcription factors can directly regulate the
Salah Boudjadi, Jean-François Beaulieu
doaj +1 more source
Improved immobilization of laccase on a glassy carbon electrode by oriented covalent attachment [PDF]
Archives of Biological Sciences, 2014 A laccase from Thermus thermophilus HB27 was reported to be potentially useful in the design of a temperature controlled biofuel cell. For enhancing its application in different thermal conditions, we engineered a laccase-oriented immobilized ...
Liu Xin, Liu Kewu
doaj +1 more source
Primers used for site-directed mutagenesis.
, 2013 Primers used for site-directed mutagenesis.
Xin Hu (38924) +6 more
core +4 more sources