Results 11 to 20 of about 465,278 (302)
SDM-assist software to design site-directed mutagenesis primers introducing "silent" restriction sites [PDF]
, 2013 BACKGROUND:Over the past decades site-directed mutagenesis (SDM) has become an indispensable tool for biological structure-function studies. In principle, SDM uses modified primer pairs in a PCR reaction to introduce a mutation in a cDNA insert.
Karnik, Abhijit +2 more
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, 2012 The first attempts to induce mutations in biological systems using chemical compounds go back to the beginning of the past century. However, it was during World War II that the two most relevant names in chemical mutagenesis, Charlotte Auerbach and Iosif A. Rapoport, established the mutagenic properties of several chemical compounds (Box 12.1).
Leitão, José
core +4 more sources
An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol [PDF]
, 2012 Background: Mutagenesis plays an essential role in molecular biology and biochemistry. It has also been used in enzymology and protein science to generate proteins which are more tractable for biophysical techniques.
Liu, Huanting, Naismith, James Henderson
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Cytotoxic and mutagenic effects of the food additive tartrazine on eukaryotic cells
BMC Pharmacology and Toxicology, 2022 Background Among the food additives used in the food industry, food dyes are considered the most toxic. For instance, tartrazine (TRZ) is a food colorant commercially available with conflicting data regarding its cytotoxic, genotoxic, and mutagenic ...
Jailson Rodrigues dos Santos +17 more
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Genes and Environment, 2020 Background (Quantitative) Structure-Activity Relationship ((Q)SAR) is a promising approach to predict the potential adverse effects of chemicals based on their structure without performing toxicological studies.
Masamitsu Honma +3 more
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Robust high-throughput assays to assess discrete steps in ubiquitination and related cascades
BMC Molecular and Cell Biology, 2020 Background Ubiquitination and ubiquitin-like protein post-translational modifications play an enormous number of roles in cellular processes. These modifications are constituted of multistep reaction cascades.
Gabriel Fenteany +5 more
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Nanomaterials, 2021 The presence of nanomaterials (NMs) in the environment may represent a serious risk to human health, especially in a scenario of chronic exposure. To evaluate the potential relationship between NM-induced epigenetic alterations and carcinogenesis, the ...
Sandra Ballesteros +6 more
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Applied Sciences, 2021 A new point of view for genetic damage assessment using the comet assay is proposed based on the number of migration groups, the number of comets in each group, and the groups with the highest number of comets. Human lymphocytes were exposed to different
Mónica Reynoso-Silva +6 more
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Pilot study of large-scale production of mutant pigs by ENU mutagenesis
eLife, 2017 N-ethyl-N-nitrosourea (ENU) mutagenesis is a powerful tool to generate mutants on a large scale efficiently, and to discover genes with novel functions at the whole-genome level in Caenorhabditis elegans, flies, zebrafish and mice, but it has never been ...
Tang Hai +58 more
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Biomolecules, 2021 Environmental plastic wastes are continuously degraded to their micro and nanoforms. Since in the environment they coexist with other pollutants, it has been suggested that they could act as vectors transporting different toxic trace elements, such as ...
Josefa Domenech +4 more
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