Results 41 to 50 of about 627,903 (299)
In vivo selection of resistant E. coli after ingestion of milk with added drug residues. [PDF]
, 2014 Antimicrobial resistance represents a major global threat to modern medicine. In vitro studies have shown that very low concentrations of drugs, as frequently identified in the environment, and in foods and water for human and animal consumption, can ...
Bicalho, Rodrigo Carvalho +3 more
core +3 more sources
In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS
FEBS Letters, EarlyView.We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka +11 more
wiley +1 more source
Genes and Environment, 2021 Background Gene mutation assays in transgenic rodents are useful tools to investigate in vivo mutagenicity in a target tissue. Using a lambda EG10 transgene containing reporter genes, gpt delta transgenic mice and rats have been developed to detect point
Kenichi Masumura +10 more
doaj +1 more source
Genetic analysis and mapping of dwarf gene without yield penalty in a γ-ray-induced wheat mutant
Frontiers in Plant Science, 2023 Plant height is one of the most important agronomic traits that affects yield in wheat, owing to that the utilization of dwarf or semi-dwarf genes is closely associated with lodging resistance.
Qingguo Wang +9 more
doaj +1 more source
Effects of single mutations on the stability of horseradish peroxidase to hydrogen peroxide [PDF]
, 2007 Horseradish peroxidase (HRP) is a commonly used enzyme in many biotechnological fields. Improvement of HRP stability would further increase its potential application range.
Arnold +18 more
core +2 more sources
Structural biology of ferritin nanocages
FEBS Letters, EarlyView.Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley +1 more source
p53 Mutations and DNA Ploidy in Colorectal Adenocarcinomas
Analytical Cellular Pathology, 1998 The p53 tumour suppressor gene has an important role in the the maintenance of genome stability and its mutational inactivation may be at the origin of aneuploidy in cancer cells.
Paola Campomenosi +12 more
doaj +1 more source
Exploiting prokaryotic chitin-binding proteins for glycan recognition [PDF]
, 2011 • The cloning, expression and characterisation of prokaryotic chitin-binding proteins from Serratia marcescens, Pseudomonas aeruginosa, Photorhabdus luminescens Microfluidics and Photorhabdus asymbiotica • Development of an assay to assess the activity
Clarke, Paul A. +4 more
core
FEBS Letters, EarlyView.This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel +13 more
wiley +1 more source
Lack of RNA-DNA oligonucleotide (chimeraplast) mutagenic activity in mouse embryos [PDF]
, 2005 There are numerous reports of the use of RNA-DNA oligonucleoticles (chimeraplasts) to correct point mutations in vitro and in vivo, including the human apolipoprotein E gene (ApoE).
Owen, JS, Simons, JP, Tagalakis, AD
core +1 more source