Results 61 to 70 of about 470,712 (310)

Functional mutation allele mining of plant architecture and yield-related agronomic traits and characterization of their effects in wheat

BMC Genetics, 2019
Background Wheat mutant resources with phenotypic variation have been developed in recent years. These mutants might carry favorable mutation alleles, which have the potential to be utilized in the breeding process.
Huijun Guo, Hongchun Xiong, Yongdun Xie, Linshu Zhao, Jiayu Gu, Shirong Zhao, Yuping Ding, Luxiang Liu   +7 more
doaj   +1 more source

Comprehensive framework between environment and genomic stability: the open symposium of the Japanese Environmental Mutagen Society (JEMS) in 2019

Genes and Environment, 2019
The open symposium of the Japanese Environmental Mutagen Society (JEMS), under the title of “Comprehensive framework between environment and genomic stability,” was held in the Main Conference Room of the Foundation for Promotion of Cancer Research ...
Katsuyoshi Horibata, Masashi Sekimoto, Kei-ichi Sugiyama   +2 more
doaj   +1 more source

Sleeping beauty transposon-mediated poly(A)-trapping and insertion mutagenesis in mouse embryonic stem cells

, 2018
Saturation mutagenesis of all endogenous genes within the mouse genome remains a challenging task, although a plenty of gene-editing approaches are available for this purpose.
Shan Zhong, Song, Guili, Li, Qing, Guili Song, Jing Ren, Zhao, Yi, Yi Zhao, Zhong, Shan, Cui, Zongbin, Ren, Jing, Zongbin Cui, Qing Li   +11 more
core   +1 more source

High-throughput Saturation Mutagenesis Screen.

, 2023
(A) A novel method for the generation of primers for high-throughput saturation mutagenesis. High-throughput chip-synthesized oligo was used to produce saturation mutagenesis primers of REGN10987 scFv.
Jianzhong Jeff Xi (155413), Ye Yang (297214), Shuo Liu (108276), Jiaxin Li (441199), Youchun Wang (284021), Junyi Wang (774099), Bolun Wang (1465285), Juan Li (146180), Buqing Ye (12568306), Yufeng Luo (827874)   +9 more
core   +1 more source

In vitro and in silico modelling of ROS1‐positive non‐small cell lung cancer reveals fusion‐dependent tyrosine kinase inhibitor responses

Molecular Oncology, EarlyView.
Drug resistance limits treatment success in a subset of lung cancers driven by ROS1 gene alterations. Using patient‐derived cells and computer simulations, we studied three key mutations and how they affect five targeted drugs. The mutations reduced drug effectiveness in different ways by altering protein structure and behavior.
Farhan Ul Haq, Marc Terrones, Felicia Rodrigues Fortes, Anne Schepers, Alessia Denis, Christophe Deben, Ken Op de Beeck, Guy Van Camp, Geert Vandeweyer   +8 more
wiley   +1 more source

Defining the optimal mutagenesis conditions (continuation).

, 2021
A. Mutagenesis rate per position using different plasmid delivery methods. B. Mutagenesis rate per position for different sgRNAs.
Estel Aparicio-Prat (3330222), Michael Bassik (11462518), Andrea Weston (11462524), Marco Mariotti (176946), Gaelen Hess (11462521), Jean-Philippe Fortin (205405), Hualin S. Xi (11462527), Dong Yan (356556), Robert Stanton (5858081)   +8 more
core   +1 more source

Paclitaxel induces NM2‐dependent cellular contraction through GEF‐H1 dissociation from microtubules and RhoA/ROCK activation in cancer cells

Molecular Oncology, EarlyView.
Taxanes are widely used chemotherapeutics whose effects on cellular mechanics remain poorly understood. We show that paclitaxel induces rapid cellular contraction by promoting GEF‐H1 dissociation from microtubules and non‐muscle myosin II activation through RhoA/ROCK.
Gloria Asensio‐Juárez, Rafael Pérez‐Díaz, Hugo Ramos‐Solano, Marina Garrido‐Casado, Vanessa C. Talayero, Miguel Vicente‐Manzanares   +5 more
wiley   +1 more source

Comparative study of cytotoxic effects induced by environmental genotoxins using XPC- and CSB-deficient human lymphoblastoid TK6 cells

Genes and Environment, 2019
Background The human genome is constantly exposed to numerous environmental genotoxicants. To prevent the detrimental consequences induced by the expansion of damaged cells, cellular protective systems such as nucleotide excision repair (NER) exist and ...
Akira Sassa, Takayuki Fukuda, Akiko Ukai, Maki Nakamura, Michihito Takabe, Takeji Takamura-Enya, Masamitsu Honma, Manabu Yasui   +7 more
doaj   +1 more source

TILLING in Cereal Crops for Allele Expansion and Mutation Detection by Using Modern Sequencing Technologies

Agronomy, 2020
A substantial increase in yield of food crops is crucial to feeding the burgeoning global population. There is a need to introduce new breeding strategies that will accelerate the average phenotypic values of crop plants.
Ahsan Irshad, Huijun Guo, Shunlin Zhang, Luxiang Liu   +3 more
doaj   +1 more source

APOBEC3 activity and DNA polymerase‐ε deficiency are associated with distinct IDH1 R132 hotspot mutations

Molecular Oncology, EarlyView.
Isocitrate dehydrogenase 1 (IDH1) mutations are highly recurrent in multiple human cancer types, including cholangiocarcinoma and glioma. IDH1 R132C is the most common IDH1 mutation in cholangiocarcinoma and likely arises from APOBEC3A‐ or APOBEC3B‐mediated deamination.
Kelly E. Butler, Bilal A. Lone, Ecem Unal, A. Rouf Banday   +3 more
wiley   +1 more source