Results 71 to 80 of about 465,278 (302)

APOBEC3 activity and DNA polymerase‐ε deficiency are associated with distinct IDH1 R132 hotspot mutations

Molecular Oncology, EarlyView.
Isocitrate dehydrogenase 1 (IDH1) mutations are highly recurrent in multiple human cancer types, including cholangiocarcinoma and glioma. IDH1 R132C is the most common IDH1 mutation in cholangiocarcinoma and likely arises from APOBEC3A‐ or APOBEC3B‐mediated deamination.
Kelly E. Butler, Bilal A. Lone, Ecem Unal, A. Rouf Banday   +3 more
wiley   +1 more source

Comparative study of cytotoxic effects induced by environmental genotoxins using XPC- and CSB-deficient human lymphoblastoid TK6 cells

Genes and Environment, 2019
Background The human genome is constantly exposed to numerous environmental genotoxicants. To prevent the detrimental consequences induced by the expansion of damaged cells, cellular protective systems such as nucleotide excision repair (NER) exist and ...
Akira Sassa, Takayuki Fukuda, Akiko Ukai, Maki Nakamura, Michihito Takabe, Takeji Takamura-Enya, Masamitsu Honma, Manabu Yasui   +7 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Mutagenesis separates ATPase and thioesterase activities of the peroxisomal ABC transporter, Comatose [PDF]

, 2019
The peroxisomal ABC transporter, Comatose (CTS), a full length transporter from Arabidopsis has intrinsic acyl-CoA thioesterase (ACOT) activity, important for physiological function.
Carrier, DJ, Rong, Hong Lin, Waterham, Hans R., Theodoulou, F. L., Wanders, r., Schaedler, TA, Baker, Alison, Waterham, HR, Baldwin, SA, Carrier, David J., Theodoulou, FL, Baldwin, Stephen A., Rong, H., Schaedler, T. A., van Roermund, CWT, Ijlist, L., Schaedler, Theresia A., IJlst, Lodewijk, Waterham, H., Wanders, Ronald J. A., Carrier, D., Rong, HL, Baldwin, S., Wanders, RJA, Baker, A, van Roermund, C., Theodoulou, Frederica L., Ijlst, L, van Roermund, Carlo W. T., Baker, A.   +29 more
core   +1 more source

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

Novel mutant alleles of the starch synthesis gene TaSSIVb-D result in the reduction of starch granule number per chloroplast in wheat

BMC Genomics, 2017
Background Transient starch provides carbon and energy for plant growth, and its synthesis is regulated by the joint action of a series of enzymes. Starch synthesis IV (SSIV) is one of the important starch synthase isoforms, but its impact on wheat ...
Huijun Guo, Yunchuan Liu, Xiao Li, Zhihui Yan, Yongdun Xie, Hongchun Xiong, Linshu Zhao, Jiayu Gu, Shirong Zhao, Luxiang Liu   +9 more
doaj   +1 more source

Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound

FEBS Open Bio, EarlyView.
We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda, Natsue Maezono, Rina Uchikoshi, Nobuyuki Kato, Masanori Ikeda   +4 more
wiley   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Biophysical characterization of mutants of Bacillus subtilis lipase evolved for thermostability: Factors contributing to increased activity retention [PDF]

, 2012
Previously, Lipase A from Bacillus subtilis was subjected to in vitro directed evolution using iterative saturation mutagenesis, with randomization sites chosen on the basis of the highest B-factors available from the crystal structure of the wild-type ...
Augustyniak, W., Augustyniak, Wojciech,, Brzezinska, Agnieszka A., Dijkstra, Bauke W., Bauke W. Dijkstra, Wienk, Hans, Wojciech Augustyniak, Brzezinska, A., Boelens, Rolf, NMR Spectroscopy, Tjaard Pijning, Wienk, H., Wienk, Hans,, Boelens, Rolf,, Pijning, Tjaard, Dijkstra, Bauke W.,, Wienk, H.L.J., Augustyniak, Wojciech, Pijning, T., Brzezinska, Agnieszka A.,, Sub NMR Spectroscopy, Boelens, R., Manfred T. Reetz, Reetz, M.T., Rolf Boelens, Agnieszka A. Brzezinska, Brzezinska, A.A., Hans Wienk, Pijning, Tjaard,, Pijning, Tjaard; id_orcid, Dijkstra, B., Reetz, M., Reetz, Manfred T.,, Reetz, Manfred T.   +33 more
core   +2 more sources

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source