Results 71 to 80 of about 352,449 (219)

SDS‐CRISPR for Single‐Nucleotide Variant Detection

Advanced Science, EarlyView.
Structure‐disruption‐sensitive CRISPR (SDS‐CRISPR) converts structural instability into single‐nucleotide precision, thereby overcoming mismatch tolerance in canonical Cas12a and enabling versatile diagnostics across DNA, RNA, and microRNA targets. When applied to rapid IDH1 mutation detection for glioma genotyping and integrated with lateral‐flow ...
Xin Guan, Chong Guo, Jiongyu Zhang, Rui Yang, Yerramsetti Ramachandra, Chengyu Hou, Minjie Pei, Shuo Zhang, Kurt T. Schalper, Xingye Liu, Qian Wu, Ketan R. Bulsara, Changchun Liu   +12 more
wiley   +1 more source

Decoupling sporulation from insecticidal activity in <i>Bacillus thuringiensis</i> BLB1 strain through Iterative UV mutagenesis. [PDF]

Front Microbiol
El Abed F, Sellami S, Escriche B, Rouis S.   +3 more
europepmc   +1 more source

Covalent Reprogramming of Kinase Binders to Modulate Protein Abundance

Advanced Science, EarlyView.
Electrophilic remodeling of a broad‐spectrum kinase binder reveals how subtle chemical changes reprogram protein fate. An acrylamide analog of a multi‐kinase binder selectively stabilizes Aurora kinase A (AURKA) by suppressing its ubiquitination, while a short‐linker variant converts this stabilizer into a degrader.
Chen Mozes, Xiaokang Jin, Miguel A. Campos, Chen Zhou, Xiaoyu Zhang   +4 more
wiley   +1 more source

Correction: Alanine-scanning mutagenesis library of MreB reveals distinct roles for regulating cell shape and viability. [PDF]

PLoS Genet
Maharjan S, Sloan R, Lusk J, Bevienguevarr R, Surber J, Morgenstein RM.   +5 more
europepmc   +1 more source

Fosl2 Regulates FSH‐Dependent Follicle Maturation Through Feedback Amplification of FSH/FSHR Signaling

Advanced Science, EarlyView.
This study identifies a FOSL2‐driven positive feedback loop that amplifies FSH/FSHR signaling. During FSH‐dependent follicle maturation, FSH induces Fosl2 expression via the cAMP‐PKA‐CREB cascade. FOSL2 in turn binds the promoters of Fshr and estrogen‐biosynthesis genes to enhance their transcription, thereby increasing Fshr mRNA level and amplifying ...
Hongru Shi, Chaoli Chen, Zaohong Ran, Jianning Liao, Zian Wu, Xiaodong Wang, Yongheng Zhao, Wenkai Ke, Bowen Tan, Yun Liu, Youqiang Su, Wei Ren, Xiang Li, Changjiu He   +13 more
wiley   +1 more source

Design and validation of an inducible and curable EvolvR system for directed evolution in <i>Corynebacterium glutamicum</i>. [PDF]

Front Bioeng Biotechnol
Will PM, Göttl VL, Seeger J, Wendisch VF, Henke NA.   +4 more
europepmc   +1 more source

Microbial Odorant Detection Guides Drosophila Parasitoids Seeking Hosts in Fermenting Fruits

Advanced Science, EarlyView.
Yeast microbes in fermenting fruits attract both host flies and their parasitoid wasps. Female Leptopilina boulardi detect yeast‐emitted ethyl esters via two olfactory receptors, LbouOR167 and LbouOR136. A conserved residue, Leu159, is critical for binding these compounds, enabling female wasps to locate host‐rich habitats.
Yueqi Lu, Lan Pang, Wenqi Shi, Ting Feng, Zhi Dong, Yifeng Sheng, Sicong Zhou, Longtao Yu, Hao Guo, Ying Wang, Jiani Chen, Jianhua Huang   +11 more
wiley   +1 more source

Effect of UV-C Radiation on Genomic Variation in <i>Chlamydomonas reinhardtii</i>. [PDF]

Genes (Basel)
Radice RP, Padula F, Iannelli V, Montagnuolo X, Scopa A, Drosos M, Martelli G.   +6 more
europepmc   +1 more source

Secreted Nonstructural Protein 3 is a Pathogenic Determinant of Orbivirus

Advanced Science, EarlyView.
This study uncovers a conserved PIP2‐dependent secretory pathway of orbivirus NS3 that induces vascular leakage. Pharmacological disruption of PIP2‐NS3 interaction significantly reduces viral pathogenicity and provides protective efficacy in murine models, establishing PIP2‐mediated NS3 secretion as both a key virulence determinant and a promising ...
Junyong Guan, Dong Zhou, Ran Shao, Xing Liu, Jin Peng, Yingran Huang, Shuhui Qi, Cankun Xi, Menghang Wang, Bin Sun, Yinglin Qi, Xin Yin   +11 more
wiley   +1 more source

Establishment of an <i>in vitro</i> pingyangmycin-induced mutagenesis system for litchi via embryogenic callus regeneration. [PDF]

Front Plant Sci
Wang G, Wang P, Liu Y, Cao X, Li F, Wang S, Li H, Wang J.   +7 more
europepmc   +1 more source