Results 31 to 40 of about 470,712 (310)

Rapid construction of mycobacterial mutagenesis vectors using ligation-independent cloning [PDF]

, 2010
Targeted mutagenesis is one of the major tools for determining the function of a given gene and its involvement in bacterial pathogenesis. In mycobacteria, gene deletion is often accomplished by using allelic exchange techniques that commonly utilise a ...
Neil G. Stoker, Stoker, N G, Francois-Xavier Chauviac, Balhana, R J C, Sikder, M H, Stoker, Neil G., Chauviac, Francois-Xavier, Sharon L. Kendall, Sikder, Mahmudul Hasan, Kendall, S L, Chauviac, F-X, Mahmudul Hasan Sikder, Kendall, Sharon L., Ricardo Balhana, Balhana, Ricardo   +14 more
core   +1 more source

Genome-Wide and Exome-Capturing Sequencing of a Gamma-Ray-Induced Mutant Reveals Biased Variations in Common Wheat

Frontiers in Plant Science, 2022
Induced mutagenesis is a powerful approach for the creation of novel germplasm and the improvement of agronomic traits. The evaluation of mutagenic effects and functional variations in crops is needed for breeding mutant strains.
Yuting Li, Yuting Li, Hongchun Xiong, Jiazi Zhang, Huijun Guo, Chunyun Zhou, Yongdun Xie, Linshu Zhao, Jiayu Gu, Shirong Zhao, Yuping Ding, Zhengwu Fang, Luxiang Liu   +12 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Structural Characterization of Agonist Binding to A3 Adenosine Receptor through Biomolecular Simulations and Mutagenesis Experiments [PDF]

, 2019
The study aimed at highlighting features of the still-unsolved A3R that are important for IB-MECA and NECA binding and may be used for the design of effective ligands using computational and mutagenesis studies.
Antonios, Kolocouris, Panagiotis, Lagarias, Graham, Ladds, Dimitrios, Stamatis, Kerry, Barkan, Eleni, Vrontaki   +5 more
core   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Genetic analysis and mapping of dwarf gene without yield penalty in a γ-ray-induced wheat mutant

Frontiers in Plant Science, 2023
Plant height is one of the most important agronomic traits that affects yield in wheat, owing to that the utilization of dwarf or semi-dwarf genes is closely associated with lodging resistance.
Qingguo Wang, Qingguo Wang, Hongchun Xiong, Huijun Guo, Linshu Zhao, Yongdun Xie, Jiayu Gu, Shirong Zhao, Yuping Ding, Luxiang Liu   +9 more
doaj   +1 more source

ENU Mutagenesis in the Mouse [PDF]

Current Protocols in Mouse Biology, 2014
AbstractThis article describes the treatment of laboratory mice with the mutagen N‐ethyl‐N‐nitrosourea (ENU) to induce very highly increased rates of mutation throughout the genome. Further, it describes several popular mating schemes designed to produce animals displaying phenotypes associated with the induced mutations. Curr. Protoc. Mouse Biol. 4:25‐
Rolf, Stottmann, David, Beier
openaire   +3 more sources

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

New homozygous gpt delta transgenic rat strain improves an efficiency of the in vivo mutagenicity assay

Genes and Environment, 2021
Background Gene mutation assays in transgenic rodents are useful tools to investigate in vivo mutagenicity in a target tissue. Using a lambda EG10 transgene containing reporter genes, gpt delta transgenic mice and rats have been developed to detect point
Kenichi Masumura, Tomoko Ando, Akiko Ukai, Sho Fujiwara, Shigeo Yokose, Xinyue You, Takayoshi Suzuki, Hiroyuki Hayashi, Takehiko Nohmi, Hisayoshi Takagi, Masamitsu Honma   +10 more
doaj   +1 more source