Label-free optical vibrational spectroscopy to detect the metabolic state of M. tuberculosis cells at the site of disease [PDF]
Tuberculosis relapse is a barrier to shorter treatment. It is thought that lipid rich cells, phenotypically resistant to antibiotics, may play a major role. Most studies investigating relapse use sputum samples although tissue bacteria may play an important role. We developed a non-destructive, label-free technique combining wavelength modulated Raman (
arxiv +1 more source
Jieying Wang,* Zhuoling Chen,* Yuni Xu, Wenhua Qiu, Shaowen Chen, Hua Pei, Yeteng Zhong Department of Clinical Laboratory, The Second Affiliated Hospital Hainan Medical University, Haikou, People’s Republic of China*These authors contributed ...
Wang J+6 more
doaj
Mycobacterium avium subspecies paratuberculosis (Map) causes chronic granulomatous disease in cattle and ruminant livestock, causing substantial economic losses.
J. Hunter Rice+3 more
doaj +1 more source
BackgroundPulmonary non-tuberculous mycobacteria (NTM) infection has become a public health concern in China and around the world. The objective of this study was to describe the longitudinal changes in the frequency and diversity of NTM in northern ...
Qing Sun+10 more
doaj +1 more source
On the ability to reconstruct ancestral genomes from Mycobacterium genus [PDF]
Technical signs of progress during the last decades has led to a situation in which the accumulation of genome sequence data is increasingly fast and cheap. The huge amount of molecular data available nowadays can help addressing new and essential questions in Evolution.
arxiv
Using Capsule Neural Network to predict Tuberculosis in lens-free microscopic images [PDF]
Tuberculosis, caused by a bacteria called Mycobacterium tuberculosis, is one of the most serious public health problems worldwide. This work seeks to facilitate and automate the prediction of tuberculosis by the MODS method and using lens-free microscopy, which is easy to use by untrained personnel.
arxiv
A single regulator NrtR controls bacterial NAD+ homeostasis via its acetylation
Nicotinamide adenine dinucleotide (NAD+) is an indispensable cofactor in all domains of life, and its homeostasis must be regulated tightly. Here we report that a Nudix-related transcriptional factor, designated MsNrtR (MSMEG_3198), controls the de novo ...
Rongsui Gao+5 more
doaj +1 more source
Analysis of DevR regulated genes in Mycobacterium tuberculosis [PDF]
The DevRS two component system of Mycobacterium tuberculosis is responsible for its dormancy in host and becomes operative under hypoxic condition. It is experimentally known that phosphorylated DevR controls the expression of several downstream genes in a complex manner.
arxiv
Elusive preferred hosts or nucleic acid level selection? A commentary on: Evolutionary interpretations of mycobacteriophage biodiversity and host-range through the analysis of codon usage bias (Esposito et al. 2016) [PDF]
While confirming the long held view that viruses do not closely imitate the use of their host's codon catalogue, Esposito and coworkers nevertheless consider it surprising that, despite having the ability to infect the same host, many mycobacteriophages share little or no genetic similarity (i.e.
arxiv
Construction and Use of Transposon MycoTetOP2 for Isolation of Conditional Mycobacteria Mutants
Mycobacteria are unique in many aspects of their biology. The development of genetic tools to identify genes critical for their growth by forward genetic analysis holds great promises to advance our understanding of their cellular, physiological and ...
Sarah D. Riggs-Shute+3 more
doaj +1 more source