Results 131 to 140 of about 6,136 (180)

Novel neuronal proteolipid protein isoforms encoded by the human myelin proteolipid protein 1 gene

Neuroscience, 2010
The human myelin proteolipid protein 1 gene (hPLP1), which encodes the major structural myelin proteins of the central nervous system (CNS), is classically described as expressed in the oligodendrocytes, the CNS myelinating cells. We identified two new exons in the intron 1 of the hPLP1 gene that lead to the expression of additional mRNA and protein ...
C, Sarret, P, Combes, P, Micheau, A, Gelot, O, Boespflug-Tanguy, C, Vaurs-Barriere   +5 more
openaire   +2 more sources

PROTEOLIPID PROTEIN: SYNTHESIS AND ASSEMBLY INTO QUAKING MOUSE MYELIN

Journal of Neurochemistry, 1979
AbstractThe incorporation of radioactive glycine into the major myelin proteolipid protein isolated from whole brain and from purified myelin of Quaking mice and normal littermates was compared. In a typical experiment, four Quaking mice and four littermate controls were injected intracranially with 250 μCi [2‐3H]glycine and 25 μCi [U‐14C]glycine ...
Greenfield, S, Williams, N I, White, M, Brostoff, S W, Hogan, E L   +4 more
openaire   +2 more sources

Radioimmunoassay for Central Nervous System Myelin‐Specific Proteolipid Protein

Journal of Neurochemistry, 1981
Abstract: A double‐antibody radioimmunoassay (RIA) has been developed with antisera to purified rat brain myelin proteolipid protein (PLP). The addition of Triton X‐100 allowed antibody‐antigen interaction and immune precipitation in the presence of sodium dodecyl sulfate (SDS).
J L, Trotter, L, Lieberman, F L, Margolis, H C, Agrawal   +3 more
openaire   +2 more sources

Pressure-induced dissociation of aggregates of myelin proteolipid protein

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1985
Sedimentation velocity and equilibrium experiments have revealed an extremely pressure-sensitive aggregation of myelin proteolipid protein in the presence of Triton X-100, dissociation of the protein aggregate being observed at pressures that are several orders of magnitude lower than those effecting disaggregation of many other proteins. These results
Gow A., Winzor D.J., Smith R.
openaire   +3 more sources

Immunolocalization of proteolipid protein peptide 103–116 in myelin

Journal of Neuroscience Research, 1994
AbstractDetermination of the topographic orientation of proteolipid protein (PLP) within myelin is part of an overall understanding of the functions of PLP and the roles of its multiple domains in diseases that primarily affect central nervous system (CNS) myelin.
Sobel, RA, Greer, JM, Isaac, J, Fondren, G, Lees, MB   +4 more
openaire   +3 more sources

Proteolipid protein interactions in transfectants: Implications for myelin assembly

Journal of Neuroscience Research, 1994
AbstractThe proteolipid proteins (PLP and DM20) are major constituents of CNS myelin, but how they are delivered to and organized within the oligodendrocyte plasma membrane is incompletely understood. We have expressed both PLP and DM20 singly or together in a host cell line, HeLa.
M. P. Sinoway, K. Kitagawa, S. Timsit, G. A. Hashim, G. A. Hashim, D. R. Colman   +5 more
openaire   +2 more sources

Spectroscopic analysis of acylated and deacylated myelin proteolipid protein

Biochemistry, 1986
The effect of covalently bound fatty acid on the conformation of the myelin proteolipid protein has been studied by ultraviolet and intrinsic fluorescence spectroscopy. With dimethyl sulfoxide used as a perturbant, the exposure of Trp and Tyr residues in various mixtures of chloroform-methanol was evaluated by difference spectroscopy of the proteolipid
O A, Bizzozero, M B, Lees
openaire   +2 more sources

Myelination in the Absence of Galactolipids and Proteolipid Proteins

Molecular and Cellular Neuroscience, 1999
The galactolipids galactocerebroside and sulfatide and the proteolipid protein (PLP) and its splice variant DM20 are the most abundant lipid and protein components of central nervous system myelin. Recent studies have found that mice lacking either the galactolipids or PLP are able to form myelin sheaths with apparently normal periodicity and near ...
Coetzee, T., Suzuki, K., Nave, K., Popko, B.   +3 more
openaire   +3 more sources

Immunogenic and encephalitogenic epitope clusters of myelin proteolipid protein

The Journal of Immunology, 1996
Abstract To understand and develop strategies to intervene in autoimmune responses to myelin proteolipid protein (PLP), encephalitogenic epitopes must be identified. To expedite the identification of potentially immunogenic and encephalitogenic epitopes of PLP, overlapping synthetic 20-mer PLP peptides covering the whole PLP molecule ...
Greer, JM, Sobel, RA, Sette, A, Southwood, S, Lees, MB, Kuchroo, VK   +5 more
openaire   +2 more sources

Increased turnover of myelin proteolipid protein in quaking mouse brain

Metabolic Brain Disease, 1987
Homozygous quaking and normal control littermate mice were injected intracerebrally with [3H]leucine at 19 days of age. The animals were sacrificed after 1 h and after 6 days. The proteolipid protein (PLP) and intermediate protein (IP) were extracted from whole brain by chloroform-methanol (2:1) and resolved by sodium dodecyl sulfate-polyacrylamide gel
G, Konat, G, Gantt, E L, Hogan
openaire   +2 more sources