Myosins XI Are Involved in Exocytosis of Cellulose Synthase Complexes [PDF]
In plants, cellulose is synthesized at the cell surface by plasma membrane (PM)-localized cellulose synthase (CESA) complexes (CSCs). The molecular and cellular mechanisms that underpin delivery of CSCs to the PM, however, are poorly understood. Cortical microtubules have been shown to interact with CESA-containing compartments and mark the site for ...
Weiwei Zhang +2 more
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Myosin XI-I is Mechanically and Enzymatically Unique Among Class-XI Myosins in Arabidopsis [PDF]
Arabidopsis possesses 13 genes encoding class-XI myosins. Among these, myosin XI-I is phylogenetically distant. To examine the molecular properties of Arabidopsis thaliana myosin XI-I (At myosin XI-I), we performed in vitro mechanical and enzymatic analyses using recombinant constructs of At myosin XI-I.
Takeshi, Haraguchi +4 more
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Arabidopsis Myosin XI: A Motor Rules the Tracks [PDF]
Plant cell expansion relies on intracellular trafficking of vesicles and macromolecules, which requires myosin motors and a dynamic actin network. Arabidopsis (Arabidopsis thaliana) myosin XI powers the motility of diverse cellular organelles, including endoplasmic reticulum, Golgi, endomembrane vesicles, peroxisomes, and mitochondria.
Chao, Cai +3 more
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Inter‐dependence of dimerization and organelle binding in myosin XI [PDF]
Summary Cytoplasmic streaming is a ubiquitous process in plant cells that is thought to be driven by the active movement of myosin XI motor proteins along actin filaments. These myosin motors bind to organelles through their C‐terminal globular tail domain, although recent studies have also suggested a role for ...
Jian-Feng, Li, Andreas, Nebenführ
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Functional Diversity of Class XI Myosins in Arabidopsis thaliana [PDF]
Plant myosin XI acts as a motive force for cytoplasmic streaming through interacting with actin filaments within the cell. Arabidopsis thaliana (At) has 13 genes belonging to the myosin XI family. Previous reverse genetic approaches suggest that At myosin XIs are partially redundant, but are functionally diverse for their specific tasks within the ...
Haraguchi, Takeshi +9 more
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Peroxisomal Localization of a Myosin XI Isoform in Arabidopsis thaliana [PDF]
The genome of Arabidopsis thaliana contains 13 myosin XI isoforms. Here we prepared a specific antibody against a peptide that mimics a unique C-terminal region from the myosin XI isoform, MYA2. The resulting antibody was used to demonstrate that MYA2 in Arabidopsis protein extracts co-sedimented with actin filaments and dissociated from the filaments ...
Kohsuke, Hashimoto +5 more
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Contribution of host intracellular transport machineries to intercellular movement of turnip mosaic virus. [PDF]
The contribution of different host cell transport systems in the intercellular movement of turnip mosaic virus (TuMV) was investigated. To discriminate between primary infections and secondary infections associated with the virus intercellular movement ...
Maxime Agbeci +4 more
doaj +1 more source
Identification and intracellular functional characterization of myosin XI and VIII in the lycophyte Selaginella moellendorffii. [PDF]
Selaginella moellendorffii myosins retain conserved intracellular functions of known plant myosins, while lycophytes possess a unique myosin VIII with an extended neck domain. Research on plant myosins has primarily focused on model species, such as green algae, mosses, and seed plants.
Tachibana K, Obara J, Tominaga M.
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Dynamics of full-length Arabidopsis myosin XI and its involvement in actin remodeling. [PDF]
Abstract Plant myosin XI plays a crucial role in intracellular transport, known as cytoplasmic streaming. Previous studies have identified associations between myosin XI and organelles by using a cargo-binding tail domain that lacks a motor domain.
Obara J, Tominaga M.
europepmc +4 more sources
Dynamics of Myosin XI: The Family Speed Demon [PDF]
Myosin XI is the fastest known processive motor, and is implicated in organelle transport and propulsion of cytoplasmic streaming in plant cells. We studied the motility and dynamics of a recombinant myosin XI motor domain hybridized with the myosin Va lever arm with 6 calmodulin-bearing IQ motifs and the dimerization motif.
Shroder, Deborah Y. +4 more
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