Results 21 to 30 of about 8,969 (152)

General approach to reversing ketol-acid reductoisomerase cofactor dependence from NADPH to NADH [PDF]

, 2013
To date, efforts to switch the cofactor specificity of oxidoreductases from nicotinamide adenine dinucleotide phosphate (NADPH) to nicotinamide adenine dinucleotide (NADH) have been made on a case-by- case basis with varying degrees of success.
Arnold, Frances H., Brinkmann-Chen, Sabine, Brustad, Eric M., Cahn, Jackson K. B., Flock, Tilman, McIntosh, John A., Meinhold, Peter, Snow, Christopher D., Zhang, Liang   +8 more
core   +1 more source

Genome-wide metabolic (re-) annotation of Kluyveromyces lactis [PDF]

, 2012
Even before having its genome sequence published in 2004, Kluyveromyces lactis had long been considered a model organism for studies in genetics and physiology.
Dias, Oscar, Ferreira, E. C., Gombert, Andreas Karoly, Rocha, I.   +3 more
core   +1 more source

Yeast cell factories for fine chemical and API production [PDF]

, 2008
This review gives an overview of different yeast strains and enzyme classes involved in yeast whole-cell biotransformations. A focus was put on the synthesis of compounds for fine chemical and API (= active pharmaceutical ingredient) production employing
Anton Glieder, Beate Pscheidt
core   +3 more sources

Engineering of CoA‐Acylating Butyraldehyde Dehydrogenase for Enhanced 1,3‐Butanediol Production in Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Microbial production of 1,3‐butanediol (1,3‐BDO) offers a renewable route to this versatile C4 chemical. However, the low performance of CoA‐acylating butyraldehyde dehydrogenase (Bld), which contains a catalytic cysteine, limits efficient production in recombinant Escherichia coli (E. coli).
Seunghyun Cho, Tayyab Islam, Sobia Islam, Junhak Lee, Sung Won Jung, Sunghoon Park   +5 more
wiley   +1 more source

Harnessing ferroptosis from multilayer defense networks to nanoplatforms for specific cancer therapy

BMEMat, EarlyView.
Nanomaterials target metabolically‐regulated ferroptosis for cancer therapy. Iron‐based or alternative nanoplatforms integrate ferroptosis with chemotherapy, immunotherapy, or radiotherapy. They enable stimulus‐responsive therapies (photothermal, photodynamic, sonodynamic) activated by near‐infrared, light, or ultrasound, achieving potent synergistic ...
Xinyue Xu, Xuejian Wang, Wenyan Jiang, Wan Hairul Anuar Kamaruddin, Chunyan Fang, Pengfei Zhang   +5 more
wiley   +1 more source

Glycolytic reprograming in Salmonella counters NOX2-mediated dissipation of ΔpH. [PDF]

, 2020
The microbial adaptations to the respiratory burst remain poorly understood, and establishing how the NADPH oxidase (NOX2) kills microbes has proven elusive. Here we demonstrate that NOX2 collapses the ΔpH of intracellular Salmonella Typhimurium.
Chakraborty, Sangeeta, Desai, Prerak, Fitzsimmons, Liam, Kim, Ju-Sim, Liu, Lin, McClelland, Michael, Porwollik, Steffen, Vazquez-Torres, Andres   +7 more
core  

Proteomic Changes in Cancer Cell Lines as a Result of Bacterial Infection

PROTEOMICS, EarlyView.
ABSTRACT Bacterial infections have been implicated in shaping the tumor microenvironment (TME), but their effects on cancer cell proteomes remain unexplored. In this study, we analyzed proteomic changes in melanoma (A375) and ovarian cancer (OVCAR3) cell line models following infection with Staphylococcus aureus strain USA300 or Salmonella enterica ...
Bo Ren, Kenneth Weke, Darryl Hardie, Mariya I. Goncheva, Ted Hupp, Javier Antonio Alfaro, Helena Pětrošová, David R. Goodlett   +7 more
wiley   +1 more source

Cofactor Fingerprinting with STD NMR to Characterize Proteins of Unknown Function: Identification of a Rare cCMP Cofactor Preference [PDF]

, 2004
Proteomics efforts have created a need for better strategies to functionally categorize newly discovered proteins. To this end, we have employed saturation transfer difference NMR with pools of closely related cofactors, to determine cofactor preferences.
Sem, Daniel, Yao, Huili
core   +1 more source

Mechanism and substrate specificity of the flavin reductase ActVB from Streptomyces coelicolor. [PDF]

, 2003
International audienceActVB is the NADH:flavin oxidoreductase participating in the last step of actinorhodin synthesis in Streptomyces coelicolor. It is the prototype of a whole class of flavin reductases with both sequence and functional similarities ...
Filisetti, Laurent, Fontecave, Marc, Niviere, Vincent   +2 more
core   +3 more sources

Uric Acid in Metabolic Dysfunction‐Associated Steatotic Liver Disease

Portal Hypertension &Cirrhosis, EarlyView.
MASLD is the most prevalent chronic liver disease worldwide, yet effective pharmacological treatments remain limited. Hyperuricemia is now recognized as a key driver of hepatic steatosis, inflammation, and fibrosis, with elevated serum uric acid levels independently predicting hepatocellular carcinoma and liver‐related mortality.
Rong Wang, Zhenyu Liu, Jun Lin, Weijing Zhang, Xianzhi Liu, Tong Zhang   +5 more
wiley   +1 more source