Results 31 to 40 of about 8,934 (160)

Quantitative Treatments for Explaining the Mechanism and Kinetics of Catalytic Electron Transfers in Murburn Processes, Particularly Involving Heme Enzymes Like (Per)oxidases and P450s. [PDF]

Biomed Res Int
The seminal Michaelis–Menten theorization for biological catalysis was based on “transition state” (TS), involving the formation of a topologically complementary substrate (S) and enzyme (E) complex (ES) at the “active site” of the latter. Rudolph Marcus put forth the theory of outer sphere electron transfer (ET) in a “donor–acceptor” TS complex, which
Manoj KM, Gideon DA, Samuel PM, Das S, Gencer N.   +4 more
europepmc   +2 more sources

Cofactor Fingerprinting with STD NMR to Characterize Proteins of Unknown Function: Identification of a Rare cCMP Cofactor Preference [PDF]

, 2004
Proteomics efforts have created a need for better strategies to functionally categorize newly discovered proteins. To this end, we have employed saturation transfer difference NMR with pools of closely related cofactors, to determine cofactor preferences.
Sem, Daniel, Yao, Huili
core   +1 more source

Identification of a Novel Self-Sufficient Styrene Monooxygenase from Rhodococcus opacus 1CP. [PDF]

, 2009
Sequence analysis of a 9-kb genomic fragment of the actinobacterium Rhodococcus opacus 1CP led to identification of an open reading frame encoding a novel fusion protein, StyA2B, with a putative function in styrene metabolism via styrene oxide and ...
Berkel, W.J.H., van, Eulberg, D., Kaschabek, S.R., Lakner, S., Schloemann, M., Tischler, D.   +5 more
core   +4 more sources

Glycolytic reprograming in Salmonella counters NOX2-mediated dissipation of ΔpH. [PDF]

, 2020
The microbial adaptations to the respiratory burst remain poorly understood, and establishing how the NADPH oxidase (NOX2) kills microbes has proven elusive. Here we demonstrate that NOX2 collapses the ΔpH of intracellular Salmonella Typhimurium.
Chakraborty, Sangeeta, Desai, Prerak, Fitzsimmons, Liam, Kim, Ju-Sim, Liu, Lin, McClelland, Michael, Porwollik, Steffen, Vazquez-Torres, Andres   +7 more
core  

Electron Transfer Pathways and Dynamics of Chloroplast NADPH-dependent Thioredoxin Reductase C (NTRC) [PDF]

, 2012
NADPH-dependent thioredoxin reductases (NTRs) contain a flavin cofactor and a disulfide as redox-active groups. The catalytic mechanism of standard NTR involves a large conformational change between two configurations.
Bernal Bayard, Pilar, Cejudo Fernández, Francisco Javier, Hervás Morón, Manuel, Navarro Carruesco, José Antonio   +3 more
core   +1 more source

Proteomic Changes in Cancer Cell Lines as a Result of Bacterial Infection

PROTEOMICS, EarlyView.
ABSTRACT Bacterial infections have been implicated in shaping the tumor microenvironment (TME), but their effects on cancer cell proteomes remain unexplored. In this study, we analyzed proteomic changes in melanoma (A375) and ovarian cancer (OVCAR3) cell line models following infection with Staphylococcus aureus strain USA300 or Salmonella enterica ...
Bo Ren, Kenneth Weke, Darryl Hardie, Mariya I. Goncheva, Ted Hupp, Javier Antonio Alfaro, Helena Pětrošová, David R. Goodlett   +7 more
wiley   +1 more source

Membrane electrochemical reactors (MER) for NADH regeneration in HLADH-catalysed synthesis: comparison of effectiveness [PDF]

, 2004
Two membrane electrochemical reactors(MER) were designed and applied to HLADH-catalysed reduction of cyclohexanone to cyclohexanol. The regeneration of the cofactor NADH was ensured electrochemically, using either methyl viologen or a rhodium complex as
Basséguy, Régine, Bergel, Alain, Délécouls-Servat, Karine   +2 more
core   +3 more sources

NQO1‐Responsive Prodrug for in Cellulo Release of Cytochalasin B as Cancer Cell‐Targeted Migrastatic

Small, EarlyView.
The design, synthesis, biochemical, and cell biological evaluation of the NQO1‐targeted prodrug BQTML‐CB is reported. BQTML‐CB selectively releases cytochalasin B upon activation by the quinone‐oxidoreductase NQO1 associated with invasiveness of cancer cells, representing a novel approach to improve cancer selectivity and reduce immunosuppressive ...
Mervic D. Kagho, Katharina Schmidt, Christopher Lambert, Lili Jia, Vignesh Venkatakrishnan, Luisa Mehr, Johan Bylund, Klemens Rottner, Marc Stadler, Theresia E. B. Stradal, Philipp Klahn   +10 more
wiley   +1 more source

The evaluation of the activity of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase under different patho-physiological conditions : a combined enzyme histochemical and biochemical approach [PDF]

, 1988
This thesis is based on a series of metabolic studies conducted on material obtained from human and experimental animal tissues and cells. The studies are essentially enzyme histochemical complemented when thought necessary with biochemical ...
Elias, E.A. (Ezzat), Heul, R.O. van der
core  

Targeting the substrate preference of a type I nitroreductase to develop antitrypanosomal quinone-based prodrugs. [PDF]

, 2012
Nitroheterocyclic prodrugs are used to treat infections caused by Trypanosoma cruzi and Trypanosoma brucei. A key component in selectivity involves a specific activation step mediated by a protein homologous with type I nitroreductases, enzymes found ...
Alsford, Alsford, Armstrong, Begleiter, Belinda S. Hall, Bern, Bienen, Bot, Brun, Bryant, Chaudhuri, Chen, Clarkson, Danson, Danson, Emma Louise Meredith, Ernster, Faeder, Fang, Fang, Fang, Fang, Garavaglia, Gascon, Goodwin, Guerra, Hall, Hall, Hall, Henderson, Hirumi, Hoet, Hu, Iyanagi, Jaiswal, Jarrom, Koder, Kubata, Müller, Nillius, Nivinskas, Nixon, O'Brien, Pal, Parkinson, Peterson, Pinto, Powis, Purkayastha, Race, Race, Ramos, Rassi, Shane R. Wilkinson, Stein, Stuart, Surve, Tatsumi, Tedeschi, Torri, Torri, Urech, Ward, Watanabe, Whiteway, Wilkinson, Yan, Zenno, Zenno   +68 more
core   +1 more source