Environmental Toxicology, EarlyView.ABSTRACT Resazurin is a cell viability phenoxazine dye widely employed for bacterial monitoring, as its colorimetric and fluorometric conversion reflects microbial metabolic activity. In this work, we demonstrate that graphene oxide (GO), a two‐dimensional nanomaterial with high surface reactivity, markedly accelerates the reduction of resazurin in the
Valentina Palmieri +2 more
wiley +1 more source
Electron Transfer Pathways and Dynamics of Chloroplast NADPH-dependent Thioredoxin Reductase C (NTRC) [PDF]
, 2012 NADPH-dependent thioredoxin reductases (NTRs) contain a flavin cofactor and a disulfide as redox-active groups. The catalytic mechanism of standard NTR involves a large conformational change between two configurations.
Bernal Bayard, Pilar +3 more
core +1 more source
The Human Cardiac “Age‐OME”: Age‐Specific Changes in Myocardial Molecular Expression
Aging Cell, EarlyView.A multi‐omics analysis was performed to uncover the molecular landscape of human cardiac ageing. We report several insights for the first time: a decrease in proteins composing the structural machinery of the heart; a likely compensatory increase in energetic flux and redox reductive capacity; and an increase in lysosomal activity.
Cassandra Malecki +20 more
wiley +1 more source
Cofactor Fingerprinting with STD NMR to Characterize Proteins of Unknown Function: Identification of a Rare cCMP Cofactor Preference [PDF]
, 2004 Proteomics efforts have created a need for better strategies to functionally categorize newly discovered proteins. To this end, we have employed saturation transfer difference NMR with pools of closely related cofactors, to determine cofactor preferences.
Sem, Daniel, Yao, Huili
core +1 more source
Glycolytic reprograming in Salmonella counters NOX2-mediated dissipation of ΔpH. [PDF]
, 2020 The microbial adaptations to the respiratory burst remain poorly understood, and establishing how the NADPH oxidase (NOX2) kills microbes has proven elusive. Here we demonstrate that NOX2 collapses the ΔpH of intracellular Salmonella Typhimurium.
Chakraborty, Sangeeta +7 more
core
Targeting the substrate preference of a type I nitroreductase to develop antitrypanosomal quinone-based prodrugs. [PDF]
, 2012 Nitroheterocyclic prodrugs are used to treat infections caused by Trypanosoma cruzi and Trypanosoma brucei. A key component in selectivity involves a specific activation step mediated by a protein homologous with type I nitroreductases, enzymes found ...
Alsford +68 more
core +1 more source
Identification of a Novel Self-Sufficient Styrene Monooxygenase from Rhodococcus opacus 1CP. [PDF]
, 2009 Sequence analysis of a 9-kb genomic fragment of the actinobacterium Rhodococcus opacus 1CP led to identification of an open reading frame encoding a novel fusion protein, StyA2B, with a putative function in styrene metabolism via styrene oxide and ...
Berkel, W.J.H., van +5 more
core +4 more sources
Cell Proliferation, EarlyView.Intracellular tryptophan levels modulate selective autophagy of FTH1 via the NCOA4 pathway. This autophagic process then inhibits 6‐HMT synthesis, which then results in an increase in reactive oxygen species and ferroptosis. ABSTRACT Hepatocellular carcinoma (HCC) remains a lethal malignancy with limited therapeutic options.
Xinxiang Cheng +10 more
wiley +1 more source
The evaluation of the activity of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase under different patho-physiological conditions : a combined enzyme histochemical and biochemical approach [PDF]
, 1988 This thesis is based on a series of metabolic studies conducted on material obtained from human and experimental animal tissues and cells. The studies are essentially enzyme histochemical complemented when thought necessary with biochemical ...
Elias, E.A. (Ezzat), Heul, R.O. van der
core
Structures and proton-pumping strategies of mitochondrial respiratory enzymes [PDF]
, 2001 Enzymes of the mitochondrial respiratory chain serve as proton pumps, using the energy made available from electron transfer reactions to transport protons across the inner mitochondrial membrane and create an electrochemical gradient used for the ...
Chan, Sunney I., Schultz, Brian E.
core +1 more source