Results 41 to 50 of about 239,596 (347)

A novel flow cytometry assay using dihydroethidium as redox-sensitive probe reveals NADPH oxidase-dependent generation of superoxide anion in human platelets exposed to amyloid peptide β

Platelets, 2019
Reactive oxygen species (ROS) generation is critical in the regulation of platelets, which has important implications in the modulation of hemostasis and thrombosis. Nonetheless, despite several assays have been described and successfully utilized in the
Aisha Alsheikh Abubaker, Dina Vara, Ian Eggleston, Ilaria Canobbio, Giordano Pula   +4 more
doaj   +1 more source

Real Time Monitoring of NADPH Concentrations in Corynebacterium glutamicum and Escherichia coli via the Genetically Encoded Sensor mBFP

Frontiers in Microbiology, 2018
Analyses of intracellular NADPH concentrations are prerequisites for the design of microbial production strains and process optimization. mBFP was described as metagenomics derived, blue fluorescent protein showing NADPH-dependent fluorescence ...
Oliver Goldbeck, Alexander W. Eck, Gerd M. Seibold, Gerd M. Seibold   +3 more
doaj   +1 more source

From omics to AI—mapping the pathogenic pathways in type 2 diabetes

FEBS Letters, EarlyView.
Integrating multi‐omics data with AI‐based modelling (unsupervised and supervised machine learning) identify optimal patient clusters, informing AI‐driven accurate risk stratification. Digital twins simulate individual trajectories in real time, guiding precision medicine by matching patients to targeted therapies.
Siobhán O'Sullivan, Lu Qi, Pierre Zalloua   +2 more
wiley   +1 more source

The cytosolic subunit p67^phox of the NADPH‐oxidase complex does not bind NADPH [PDF]

FEBS Letters, 2009
The NADPH‐oxidase of phagocytic cells is a multicomponent enzyme that generates superoxide. It comprises a membrane flavocytochrome b 558 and four cytosolic proteins; p67phox, p47phox, p40phox and Rac. The NADPH‐binding site of this complex was shown to be located on the flavocytochrome b 558. However, a number of studies have suggested the presence of
Baciou, Laura, Erard, Marie, Dagher, Marie-Claire, Bizouarn, Tania, Tania.Bizouarn@u-Psud.Fr   +3 more
openaire   +4 more sources

Actin dynamics controlled by IqgC, a RasGAP at the crossroads between the IQGAP and fungal GAP1 families

FEBS Open Bio, EarlyView.
IqgC is a RasGAP from Dictyostelium discoideum. IqgC binds RasG via its RasGAP domain and deactivates it on macroendocytic cups, thereby suppressing the uptake of fluid and particles. IqgC has a positive effect on cell‐substratum adhesion, and its RGCt domain is required for recruitment to ventral foci.
Vedrana Filić, Darija Putar, Lucija Mijanović, Igor Weber   +3 more
wiley   +1 more source

The roles of NADPH oxidase in modulating neutrophil effector responses

Molecular Oral Microbiology, 2019
Neutrophils are phagocytic innate immune cells essential for killing bacteria via activation of a wide variety of effector responses and generation of large amounts of reactive oxygen species (ROS).
M. Zeng, Irina Miralda, C. L. Armstrong, S. Uriarte, J. Bagaitkar   +4 more
semanticscholar   +1 more source

NADPH Oxidases in Aortic Aneurysms

Antioxidants, 2022
Abdominal aortic aneurysms (AAAs) are a progressive dilation of the infrarenal aorta and are characterized by inflammatory cell infiltration, smooth muscle cell migration and proliferation, and degradation of the extracellular matrix. Oxidative stress and the production of reactive oxygen species (ROS) have been shown to play roles in inflammatory cell
openaire   +3 more sources

Phosphomimetic mutations near active sites of proteins in Thermus thermophilus suggest a widespread regulatory mechanism

FEBS Open Bio, EarlyView.
In Thermus thermophilus, more than half of the phosphorylation sites identified by proteomic analysis are located near the active site. All phosphomimetic mutants of phosphosites of six enzymes belonging to different families showed severely reduced activity compared with the wild‐type, particularly in the turnover number.
Anzu Nishiwaki, Hiroki Okanishi, Yoshikatsu Kanai, Ryoji Masui   +3 more
wiley   +1 more source

Pharmacological and genetic inhibition of NADPH oxidase does not reduce brain damage in different models of perinatal brain injury in newborn mice

Neurobiology of Disease, 2008
Background: Inflammation and reactive oxygen species (ROS) are important in the development of perinatal brain injury. The ROS-generating enzyme NADPH oxidase (Nox2) is present in inflammatory cells and contributes to brain injury in adult animal models.
Christina Doverhag, Matthias Keller, Anna Karlsson, Maj Hedtjarn, Ulf Nilsson, Edith Kapeller, Gergely Sarkozy, Lars Klimaschewski, Christian Humpel, Henrik Hagberg, Georg Simbruner, Pierre Gressens, Karin Savman   +12 more
doaj   +1 more source

NADPH oxidase activation in neutrophils: Role of the phosphorylation of its subunits

European Journal of Clinical Investigation, 2018
Neutrophils are key cells of innate immunity and during inflammation. Upon activation, they produce large amounts of superoxide anion (O2−.) and ensuing reactive oxygen species (ROS) to kill phagocytized microbes.
S. Belambri, Loïc Rolas, Houssam Raad, M. Hurtado-Nedelec, P. M. Dang, J. El-Benna   +5 more
semanticscholar   +1 more source