Results 181 to 190 of about 19,829 (313)

Allosteric inhibition of VIM metallo-β-lactamases by a camelid nanobody [PDF]

, 2013
Jean Sohier, Clémentine Laurent, Andy Chevigné, Els Pardon, Vasundara Srinivasan, U. Wernery, Patricia Lassaux, Jan Steyaert, Moreno Galleni   +8 more
openalex   +1 more source

Penta‐ALFA‐Tagged Substrates for Self‐Labelling Tags Allow Signal Enhancement in Microscopy

Journal of Peptide Science, Volume 31, Issue 5, May 2025.
A pentaALFA peptide may be reacted with a SNAP‐ or HaloTag and allows for signal enhancement using fluorescently labelled nanobodies. Tagged and peptide/nanobody treated cell surface receptors give generally higher signal intensity using fluorescence microscopy and are amenable for stimulated emission by depletion (STED) super‐resolution imaging ...
Souvik Ghosh, Ramona Birke, Ashwin Karthick Natarajan, Johannes Broichhagen   +3 more
wiley   +1 more source

SARS-CoV-2 nanobodies 2.0 [PDF]

Signal Transduction and Targeted Therapy, 2021
Fabien Labroussaa, Joerg Jores
openaire   +5 more sources

Structure of a nanobody-stabilized active state of the β2 adrenoceptor

, 2011
Søren G. F. Rasmussen, Hee‐Jung Choi, Juan José Fung, Els Pardon, Paola Casarosa, Pil Seok Chae, Brian T. DeVree, Daniel M. Rosenbaum, Foon Sun Thian, Tong Sun Kobilka, Andreas Schnapp, Ingo Konetzki, Roger K. Sunahara, Samuel H. Gellman, Alexander Pautsch, Jan Steyaert, William I. Weis, Brian K. Kobilka   +17 more
openalex   +2 more sources

Stabilizing, camelid-derived nanobodies to facilitate generation of crystal structures of GPCRs bound to low-affinity, natural ligands [PDF]

, 2013

openalex   +1 more source

Characterisation and correction of polarisation effects in fluorescently labelled fibres

Journal of Microscopy, Volume 298, Issue 2, Page 185-203, May 2025.
Summary Many biological structures take the form of fibres and filaments, and quantitative analysis of fibre organisation is important for understanding their functions in both normal physiological conditions and disease. In order to visualise these structures, fibres can be fluorescently labelled and imaged, with specialised image analysis methods ...
Nandini Aggarwal, Richard Marsh, Stefania Marcotti, Tanya J Shaw, Brian Stramer, Susan Cox, Siân Culley   +6 more
wiley   +1 more source

A method for rapid nanobody screening with no bias of the library diversity

iScience
Summary: Nanobody, referred to the variable domain of heavy-chain-only antibodies, has several advantages such as small size and feasible Escherichia coli expression, making them promising for scientific research and therapies.
Zhiqing Tao, Xiaoling Zhao, Huan Wang, Juan Zhang, Guosheng Jiang, Bin Yu, Yihao Chen, Mingjun Zhu, Junli Long, Lei Yin, Xu Zhang, Maili Liu, Lichun He   +12 more
doaj  

Development of Cys38 knock-out and humanized version of NbAahII10 nanobody with improved neutralization of AahII Scorpion toxin

, 2011
Rahma Ben Abderrazek, Cécile Vincke, Issam Hmila, Dirk Saerens, Naima Abidi, M. El Ayeb, Serge Muyldermans, Balkiss Bouhaouala‐Zahar   +7 more
openalex   +1 more source

Editing of an antiviral host factor boosts plant growth and yield of plant viral vector‐mediated heterologous protein expression

Plant Biotechnology Journal, EarlyView.
Zhu Fang, Xinru Zhao, Min Du, Xinyi Xu, Hui Zhou, Wei Guo, Xueping Zhou, Xiuling Yang   +7 more
wiley   +1 more source

Modular Calcium‐Responsive and CD9‐Targeted Phospholipase System Enhancing Endosomal Escape for DNA Delivery

Advanced Science, Volume 12, Issue 15, April 17, 2025.
A modular endosomal escape system boosts transfection efficiency of a DNA delivery agent. This system comprises a phospholipase fused to a split‐calbindin domain and a nanobody targeting CD9. In the extracellular space, the phospholipase is tightly bound to protein‐DNA complexes, released within endosomes at low calcium concentrations and recruited to ...
Alexander Klipp, Christina Greitens, David Scherer, Alexander Elsener, Jean‐Christophe Leroux, Michael Burger   +5 more
wiley   +1 more source