Results 21 to 30 of about 56,636 (249)

Soil Metatranscriptomics: An Improved RNA Extraction Method Toward Functional Analysis Using Nanopore Direct RNA Sequencing

Phytobiomes Journal, 2023
Soil microbes play an undeniable role in sustainable agriculture, plant health, and soil management. A deeper understanding of soil microbial composition and function has been gained through next-generation sequencing.
Abdonaser Poursalavati, Vahid J. Javaran, Isabelle Laforest-Lapointe, Mamadou L. Fall   +3 more
doaj   +1 more source

Stable fabrication of a large nanopore by controlled dielectric breakdown in a high-pH solution for the detection of various-sized molecules

Scientific Reports, 2019
For nanopore sensing of various-sized molecules with high sensitivity, the size of the nanopore should be adjusted according to the size of each target molecule.
Itaru Yanagi, Rena Akahori, Ken-ichi Takeda   +2 more
doaj   +1 more source

DNAzyme Sensor for the Detection of Ca²⁺ Using Resistive Pulse Sensing

Sensors, 2020
DNAzymes are DNA oligonucleotides that can undergo a specific chemical reaction in the presence of a cofactor. Ribonucleases are a specific form of DNAzymes where a tertiary structure undergoes cleavage at a single ribonuclease site.
Imogen Heaton, Mark Platt
doaj   +1 more source

LAFITE Reveals the Complexity of Transcript Isoforms in Subcellular Fractions

Advanced Science, 2023
Characterization of the subcellular distribution of RNA is essential for understanding the molecular basis of biological processes. Here, the subcellular nanopore direct RNA‐sequencing (DRS) of four lung cancer cell lines (A549, H1975, H358, and HCC4006)
Jizhou Zhang, Xiao Lin, Yuelong Chen, Tsz‐Ho Li, Alan Chun‐Kit Lee, Eugene Yui‐Ching Chow, William Chi‐Shing Cho, Ting‐Fung Chan   +7 more
doaj   +1 more source

Barcode demultiplexing of nanopore sequencing raw signals by unsupervised machine learning

Frontiers in Bioinformatics, 2023
Introduction: Oxford Nanopore Technologies (ONT) is a third generation sequencing approach that allows the analysis of individual, full-length nucleic acids. ONT records the alterations of an ionic current flowing across a nano-scaled pore while a DNA or
Daniele M. Papetti, Simone Spolaor, Simone Spolaor, Iman Nazari, Iman Nazari, Andrea Tirelli, Andrea Tirelli, Tommaso Leonardi, Chiara Caprioli, Chiara Caprioli, Daniela Besozzi, Daniela Besozzi, Thalia Vlachou, Pier Giuseppe Pelicci, Pier Giuseppe Pelicci, Paolo Cazzaniga, Paolo Cazzaniga, Marco S. Nobile, Marco S. Nobile, Marco S. Nobile   +19 more
doaj   +1 more source

The impact of Hnrnpl deficiency on transcriptional patterns of developing muscle cells

FEBS Open Bio, EarlyView.
We performed nanopore whole‐transcriptome sequencing comparing RNA from Hnrnpl‐knockdown versus control C2C12 myoblasts to investigate the contributions of Hnrnpl to muscle development. Our results indicate that Hnrnpl regulates the expression of genes involved with Notch signaling and skeletal muscle, particularly splicing patterns of specific muscle ...
Hannah R. Littel, Mekala Gunasekaran, Audrey L. Daugherty, Natalya M. Wells, Johnnie Turner, Christine C. Bruels, Christina A. Pacak, Isabelle Draper, Peter B. Kang   +8 more
wiley   +1 more source

High resolution long-read telomere sequencing reveals dynamic mechanisms in aging and cancer

Nature Communications
Telomeres are the protective nucleoprotein structures at the end of linear eukaryotic chromosomes. Telomeres’ repetitive nature and length have traditionally challenged the precise assessment of the composition and length of individual human telomeres ...
Tobias T. Schmidt, Carly Tyer, Preeyesh Rughani, Candy Haggblom, Jeffrey R. Jones, Xiaoguang Dai, Kelly A. Frazer, Fred H. Gage, Sissel Juul, Scott Hickey, Jan Karlseder   +10 more
doaj   +1 more source

A review and analysis of current-mode biosensing front-end ICs for nanopore-based DNA sequencing

Frontiers in Electronics, 2022
Bio-sensors connect the biological world with electronic devices, widely used in biomedical applications. The combination of microelectronic and medical technologies makes biomedical diagnosis more rapid, accurate, and efficient.
Xu Liu, Qiumeng Fan, Zhijie Chen, Peiyuan Wan, Wei Mao, Hao Yu   +5 more
doaj   +1 more source

Interplay between RNA‐protein interactions and RNA structures in gene regulation

FEBS Open Bio, EarlyView.
Methodological advances in mapping transcriptome‐wide RNA‐protein interactions and RNA structures have started to uncover the potential of RNP conformations in gene regulation. Competing RNA–RNA, RNA‐protein and protein–protein interactions shape the compaction and function of RNPs throughout their lifetime and may provide novel therapeutic targets in ...
Jenni Rapakko, Mauro Scaravilli, Minna‐Liisa Änkö   +2 more
wiley   +1 more source

Practice Recommendations for Genetic Testing of Ataxias

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Over the past decade, significant advances in genetic testing for ataxia have improved diagnostic accuracy, informed clinical trial eligibility, guided treatment decisions, and enabled cascade testing of at‐risk relatives. While guidance exists for other neurogenetic conditions, there are no standardized guidelines on genetic ...
Sharan R. Srinivasan, Amy D. Mook, Michelle Rochman, Jin Yun Helen Chen, Weiyi Mu, George R. Wilmot, Liana S. Rosenthal, Wendy R. Uhlmann   +7 more
wiley   +1 more source