Results 231 to 240 of about 743,554 (284)

From fluke to fragment: A multifaceted method for molecular sex identification and mitochondrial haplotyping from environmental DNA samples

Methods in Ecology and Evolution, EarlyView.
Abstract Environmental DNA (eDNA) analyses have become a powerful tool for non‐invasive biodiversity monitoring, yet the applicability of population‐genetic approaches to environmental samples remains largely unexplored. Even when genetic traces originate from a single individual, low target DNA concentrations and amplification or sequencing artefacts ...
Lauren Kelly Rodriguez, Sandra Schallhart, Philipp Hobmeier, Thomas G. Curran, Sergi Pérez‐Jorge, Rui Prieto, Cláudia Oliveira, Mónica A. Silva, Bettina Thalinger   +8 more
wiley   +1 more source

Systematic benchmarking of dorado basecalling models for RNA modification detection with highly multiplexed nanopore sequencing. [PDF]

Nucleic Acids Res
Diensthuber G, Milenkovic I, Llovera L, Milovanovic A, Pelizzari F, Novoa EM.   +5 more
europepmc   +1 more source

Enhanced fish detection sensitivity in aquatic systems using environmental RNA

Methods in Ecology and Evolution, EarlyView.
Abstract Environmental RNA (eRNA) can potentially augment the biological monitoring ability of the established environmental DNA (eDNA) application in conservation. Despite faster degradation, environmental RNA could offer unique advantages: it may help reduce the legacy signal compared to eDNA, provide insights into physiological status through gene ...
Ana Ramón‐Laca, Ramón Gallego, Carmen Duque, José L. J. Ledesma, Ignacio Doadrio, Kim M. Parsons, Krista M. Nichols, Samantha Engster, Abigail Wells, Ana Riesgo   +9 more
wiley   +1 more source

High-throughput detection of microeukaryotic parasites in insects using Nanopore sequencing. [PDF]

Microb Genom
Bessette E, Edwards S, Williams BAP.
europepmc   +1 more source

Interlaboratory performance testing on EPIC v2.0 CNS tumor profiling demonstrates high reproducibility of tumor classification but reveals the need for harmonized copy number variation reporting

Brain Pathology, EarlyView.
Using the Infinium MethylationEPIC v2.0 array and Heidelberg Brain Tumor Classifier v12.8, 24 international laboratories achieved highly reproducible CNS tumor classification (97.9% correct; median β‐correlation r = 0.99), while copy number variation interpretation showed substantial interlaboratory variability, highlighting the need for harmonized CNV
Katrin Mauch‐Mücke, Christin Siewert, Lora Dimitrova, Sandra Pohl, Brendan Osberg, Annika K. Wefers, Julia Lorenz, Tanja Rothhammer‐Hampl, Till Acker, Alonso Barrantes‐Freer, Jean Boutonnat, Bérengère Dadone‐Montaudié, Claire Delbridge, Wolfgang Dietmaier, Sebastian Dintner, Hildegard Dohmen, Matthias Dottermusch, Daniel Erny, Katrin B. M. Frauenknecht, Tobias Goschzik, Patrick N. Harter, Daniela Hirsch, Yanghao Hou, Theo F. J. Kraus, Maggie Lee, Friederike Liesche‐Starnecker, Anne Mc Leer, Linea Melchior, Michael Mittelbronn, Wolf C. Müller, Shalima S. Nair, Julia E. Neumann, Pitt Niehusmann, Nicole Pfarr, Torsten Pietsch, Marco Prinz, Markus Rechsteiner, Regina Reimann, Laveniya Satgunaseelan, Veronica Skarpeteig, Anne J. Skjulsvik, Karl Sotlar, Isabelle Vanden Bempt, Erik Vassella, Katharina J. Weber, Gabriel Wasinger, Andreas Waha, Karen Zwaenepoel, Felix Sahm, Markus J. Riemenschneider, David Capper   +50 more
wiley   +1 more source

Oxford Nanopore Sequencing in pediatric emergency infectious diseases: from rapid diagnosis to precision medicine. [PDF]

Front Cell Infect Microbiol
Guo Y, Cheng A, Huang Y.
europepmc   +1 more source

Clinical evaluation of the CrossNN DNA methylation classifier for central nervous system tumors

Brain Pathology, EarlyView.
CrossNN demonstrated non‐inferiority to the Heidelberg CNS Tumor Methylation Classifier. A dual‐classifier approach increased the number of clinically informative classifications with correct tumor type assignment while maintaining a low rate of incorrect predictions.
Jonas Dahnoun, Léon C. van Kempen, Senada Koljenović, Ken Op de Beeck, Tomas Menovsky, Bart Feyen, Melek Ahmed, Anne Sieben, Karen Zwaenepoel   +8 more
wiley   +1 more source

Evaluating the detection capabilities of nanopore sequencing for Borrelia burgdorferi detection in blacklegged ticks. [PDF]

Sci Rep
Cassens J, Kipp EJ, Frank LE, Larsen PA, Oliver JD.   +4 more
europepmc   +1 more source

Improved Cas9-targeted nanopore sequencing facilitates ultra-deep analysis of genomic variation. [PDF]

Cell Rep Methods
Vereecke N, Behrmann M, Khare A, Dekker JP.   +3 more
europepmc   +1 more source