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The Use of the Internal Transcribed Spacer Region for Phylogenetic Analysis of the Microsporidian Parasite Enterocytozoon hepatopenaei Infecting Whiteleg Shrimp (Penaeus vannamei) and for the Development of a Nested PCR as Its Diagnostic Tool. [PDF]

J Microbiol Biotechnol
Lee JH, Jeon HJ, Seo S, Lee C, Kim B, Kwak DM, Rhee MH, Piamsomboon P, Nuraini YL, Je CU, Park SY, Kim JH, Han JE.   +12 more
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Application of nested PCR for diagnosis of histoplasmosis

Journal of Infection and Chemotherapy, 2013
Histoplasmosis is a fungal infection that, although not endemic in Japan, has seen a rise in the number of Japanese cases since the mid-1980s. Diagnosis of the disease is not straightforward, and the main method of detection, fungal culture (which has biosafety-related issues), is of low sensitivity in general.
Hideaki Ohno, Koichi Tanabe, Takashi Umeyama, Yukihiro Kaneko, Satoshi Yamagoe, Yoshitsugu Miyazaki, Hideaki Ohno, Koichi Tanabe, Takashi Umeyama, Satoshi Yamagoe   +9 more
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Nested PCR for the Detection of Cryptosporidium parvum

Parasitology Today, 1999
In their recent review, Morgan and Thompson1xMorgan, U.M. and Thompson, R.C.A. Parasitol. Today. 1998; 14: 241–245Abstract | Full Text | Full Text PDF | PubMed | Scopus (48)See all References1 described the pitfalls of using nested PCR for the detection of the diarrhoea-causing protozoan, Cryptosporidium parvum, in faecal samples or in waste or surface
C L, Gibbons, F M, Awad-El-Kariem
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Nested Polymerase Chain Reaction (PCR)

Cold Spring Harbor Protocols, 2019
Nested polymerase chain reaction (PCR) is used in situations in which it is necessary to increase the sensitivity and/or specificity of PCR, for example, when amplifying a particular member of a polymorphic gene family or when amplifying a cDNA copy of an mRNA present at very low abundance in a clinical specimen containing a heterogeneous population of
Michael R, Green, Joseph, Sambrook
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Comparison of the Specificity and Sensitivity of PCR, Nested PCR, and Real-Time PCR for the Diagnosis of Histomoniasis

Avian Diseases, 2005
Blackhead, also known as enterohepatitis, is caused by a protozoan parasite called Histomonas meleagridis. Clinical symptoms are nonspecific. Until now, diagnosis has been mainly based on postmortem lesions and microscopical and histopathological examination.
H M, Hafez, R, Hauck, D, Lüschow, L, McDougald   +3 more
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Application of nested-PCR technique for the diagnosis of tuberculosis

Bulletin of Experimental Biology and Medicine, 2000
A test-system based on amplification of IS 986 fragment (nested-PCR) was developed for the detection of Mycobacterium tuberculosis and M. bovis in different biological samples. We constructed external primers and selected appropriate amplifications parameters (annealing temperatures for states I and II, the number of cycles for each amplification stage,
O I, Skotnikova, A Y, Sobolev, V V, Demkin, N P, Nikolaeva, E Y, Nosova, E L, Isaeva, A M, Moroz, V I, Litvinov   +7 more
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NESTED-PCR ASSAYS FOR DETECTION OF PHYTOPLASMAS IN STRAWBERRY

Acta Horticulturae, 1997
Flower and fruit virescence and phyllody are known to be associated with the presence of phytoplasmas in symptomatic tissues. During surveys in North and South Italy symptomatic and asymptomatic strawberry samples were examined by nested polymerase chain reaction (PCR) to verify the presence of phytoplasmas using 16S ribosomal DNA primers.
Bertaccini A., Vibio M., Pastore M., Recupero S., Guerrini S., Grimaldi D.   +5 more
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Detection of bovine gammaherpesviruses by a nested duplex PCR

Journal of Virological Methods, 2004
The two bovine gammaherpesviruses (Alcelaphine herpesvirus 1, AlHV-1; Bovine herpesvirus 4, BoHV-4) are distributed worldwide in cattle populations. Since the animals are frequently infected latently with no or low seropositivity, a DNA based diagnostic method would be useful for surveys and detection of these viruses.
Katalin, Fábián, László, Egyed
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Multiplex nested PCR for Brazilian Alphavirus diagnosis

Transactions of the Royal Society of Tropical Medicine and Hygiene, 2004
A reverse-transcriptase PCR (RT-PCR) and a multiplex nested PCR were developed for the rapid detection and identification of 14 Brazilian alphaviruses. Using Alphavirus genus-specific primers in a RT-PCR, we obtained amplified products of 434 bp. Species-specific primers were selected and simultaneously tested in a multiplex nested PCR.
Roberta V M, Bronzoni, Marcos L, Moreli, Ana Cecília R, Cruz, Luiz Tadeu M, Figueiredo   +3 more
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Detection of HPV Transcripts by Nested RT-PCR

2005
Human papillomaviruses (HPVs) are etiologic for the development of cervical cancer and its precursor lesions, cervical intraepithelial neoplasia (CIN). Nearly all cervical carcinomas (CaCx) harbor HPV DNA, but the presence of HPV alone is not indicative of the future development of neoplasia.
Christine, Mant, Barbara, Kell, John, Cason   +2 more
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