Results 151 to 160 of about 982,945 (306)

Rapid subgroup identification of the flaviviruses using degenerate primer E-gene RT-PCR and site specific restriction enzyme analysis.

, 2005
A simplified and rapid method for the diagnosis of all flaviviruses could provide an important tool for understanding their epidemiology. A protocol based on the use of degenerate nested oligonucleotide primers and RT-PCR was developed for the ...
Gould, EA, Gaunt, MW
core   +1 more source

Integrated one‐pot CRISPR‐Cas detection: Addressing strategies and future prospects

Interdisciplinary Medicine, EarlyView.
Integrated one‐pot CRISPR‐Cas detection significantly simplifies workflows, reduces cross‐contamination risks, and shortens turnaround times, making it highly suitable for point‐of‐care testing (POCT) and resource‐limited settings. Key challenges include system compatibility issues between Cas proteins and isothermal amplification, limited multiplexing
Jian Zhou, Xuemei Ren, Yingjie Ren, Ruiping Wu, Ruiman Geng, Le Chen, Zhuo Li   +6 more
wiley   +1 more source

Testing for Non-nested Conditional Moment Retrictions via Conditional Empirical Likelihood [PDF]

We propose non-nested tests for competing conditional moment resctriction models using a method of empirical likelihood. Our tests are based on the method of conditional empirical likelihood developed by Kitamura, Tripathi and Ahn (2004) and Zhang and ...
Taisuke Otsu, Yoon-Jae Whang
core  

Offspring Global DNA Methylation is not Affected by Parental Developmental Stress Experience in a Passerine Species

Journal of Experimental Zoology Part A: Ecological and Integrative Physiology, EarlyView.
ABSTRACT Rapid phenotypic change may be mediated by intergenerational transmission of early developmental experiences. In birds, exposure to glucocorticoids during early development can alter global DNA methylation and gene expression. However, DNA methylation is labile, and it is currently unclear whether the impacts of stress suffered during juvenile
Anna Miltiadous, Harrison J. F. Eyck, Ondi L. Crino, Lee A. Rollins, Damien Callahan, Katherine L. Buchanan   +5 more
wiley   +1 more source

[Use of RT-PCR and nested PCR for diagnosing enterovirus infections].

Medycyna doswiadczalna i mikrobiologia, 2000
An application of reverse transcription (RT-PCR) and nested-PCR for the detection of human enteroviruses isolated in Poland is described. A specific 297-bp fragment was amplified by the method from all studied enteroviruses strains. Amplification system and the nested primers allowed to detect RNA isolated from 10(1) TCID50/ml which corresponded to one
I, Binduga-Gajewska, W, Gut, A, Wielkopolska, Z, Jarzabek   +3 more
openaire   +1 more source

Periodontitis and gastric disorders: Systematic review and meta‐analysis of functional dyspepsia and Helicobacter pylori gastritis

Journal of Periodontology, EarlyView.
Abstract Background Periodontitis has been associated with other systemic inflammatory diseases, such as cardiovascular disease, diabetes mellitus, and rheumatoid arthritis; through the translocation of oral bacteria and local inflammatory mediators systemically.
Hadeel Mohammed Abbood, Jasim Mohammed Abbood, Ekin Yay‐Kus, Iain L. C. Chapple   +3 more
wiley   +1 more source

Heterogeneity Analyzed by CT‐Based Habitat Analysis for Clinical Management of Cancers: A Narrative Review

The Kaohsiung Journal of Medical Sciences, EarlyView.
ABSTRACT Tumors are highly heterogeneous, and whole‐lesion radiomics analysis is a popular method for extracting texture features that reflect this heterogeneity, which can be used to build models for cancer diagnosis, staging, therapy response evaluation, and prognosis prediction.
Yang Zhang, Zhi‐Yan Jin, Min‐Ying Lydia Su   +2 more
wiley   +1 more source

Pretreatment Prediction of Tumor Recurrence in Breast Cancer After Neoadjuvant Systemic Therapy Using Machine Learning With Clinical and CT Radiomics Features

The Kaohsiung Journal of Medical Sciences, EarlyView.
ABSTRACT This study aimed to develop machine learning models for predicting tumor recurrence in breast cancer before neoadjuvant systemic therapy (NST) by integrating clinical and radiomic features derived from pretreatment computed tomography (CT).
Huei‐Yi Tsai, Jo‐Ching Wang, Wei‐Shiuan Chung, Jui‐Sheng Hsu, Shu‐Yen Lin, Ming‐Feng Hou, Ming‐Chung Chou   +6 more
wiley   +1 more source

Molecular identification of Anaplasma platys in cattle by nested PCR. [PDF]

Iran J Microbiol, 2023
Al-Saadi M, Al-Sallami D, Alsultan A.
europepmc   +1 more source

Methylation Specific PCR (MSP): Nested PCR vs Unnested PCR

, 2023
Farizky Martriano Humardani, Lisa Thalia Mulyanata, Lady Theresa Adeodata Tanaya, Risma Ikawaty, Heru Wijono, Hikmawan Wahyu Sulistomo, Dini Kesuma, Sulistyo Emantoko Dwi Putra   +7 more
openaire   +1 more source