Results 161 to 170 of about 982,945 (306)

Nested-PCR for detection of Brucella in milk

浙江大学学报. 农业与生命科学版, 2008
To detect Brucella inflection in milk, a nested-PCR assay was developed by designing outward-directed and inward-directed primers for omp25, omp31 and 16S rRNA genes of Brucella abortus, and optimizing the extraction of Brucella genomic DNA. The results show that two rounds of amplification with the two pairs of synthesized primers F4/R2 and F2D/R1U ...
DU Zhen-kun, GUO Jun-qing, ZHANG Miao-xian, XIANG Yu-yan, ZHOU Ji-yong   +4 more
openaire   +1 more source

Resilience Pathways for Land Degradation Neutrality: How Informal Institutions Shape Community Land Governance Across the Global South

Land Degradation &Development, EarlyView.
ABSTRACT A decade after Land Degradation Neutrality was incorporated into Sustainable Development Goal 15.3, implementation outcomes across the Global South remain uneven. Mainstream commons research identifies recurring institutional features, whereas critical institutionalism emphasizes their adaptive assembly; both traditions increasingly recognize ...
Tengyuan Yang, Senbao Lu, Jiapeng Dai
wiley   +1 more source

Diagnóstico temprano en un brote epidémico del virus Dengue en Piura usando RT-PCR y nested-PCR

Revista Peruana de Medicina Experimental y Salud Pública, 1997
Un test de diagnóstico temprano (RT-PCR y Nested-PCR) fue evaluado y comparado con métodos convencionales (cultivo in vitro, IFI y MAC-ELISA). Treinta y cuatro sueros de pacientes correspondientes de un brote epidémico de la costa norte peruana (Mancora,
Oscar Nolasco, Carlos Carrillo, Victoria Gutierrez, Carlos Yabar, Susan Douglas, María García, Ysabel Montoya   +6 more
doaj  

Nested PCR [PDF]

, 2020
openaire   +1 more source

Is DNA metabarcoding an option for formaldehyde‐preserved zooplankton time series?

Limnology and Oceanography: Methods, EarlyView.
Abstract The recovery of amplifiable DNA from formaldehyde‐fixed (FF) zooplankton samples has long been considered problematic, but advances in degraded‐DNA retrieval have renewed interest in FF samples. To access the information stored in long‐term zooplankton time series, we evaluated methods for extracting amplifiable DNA from community samples ...
Aitor Albaina, Anders Lanzén, Irati Miguel, Fernando Rendo, María Santos   +4 more
wiley   +1 more source

Unshared on the top: strong segregation of pathogen communities between western honey bees and wild bees in a retreating glacier foreland

Oikos, EarlyView.
Shared flowers can facilitate pathogen transmission between managed honey bees and wild bees, yet this process remains poorly understood in ecosystems undergoing rapid environmental change. Using a glacier foreland in Switzerland, we present the first study on how glacier retreat stages and bee life‐history traits influence pathogen occurrence across ...
Andrea Ferrari, Giovanni Cilia, Matteo Conti, Bao Ngan Tu, Nora Khelidj, Gianalberto Losapio, Carlo Polidori   +6 more
wiley   +1 more source

An Integrated Statistical and Machine Learning Framework for Predicting the Dry Sliding Tribological Behavior of CFRP and GFRP Composites Under Environmental Aging and Loading Conditions

Polymer Composites, EarlyView.
The graphical abstract presents the experimental and data‐driven framework used to investigate the dry sliding tribological behavior of CFRP and GFRP composites. The effects of seawater, engine oil, diesel fuel, aging time, and normal load on the specific wear rate (SWR) and coefficient of friction (COF) were evaluated using pin‐on‐disc tests, ANOVA ...
Ahmet Saylık, İhsan Tuğal, Şemsettin Temiz   +2 more
wiley   +1 more source

Nested-PCR [PDF]

, 2018
openaire   +2 more sources

Exploring eco‐evolutionary and temporal patterns of arbuscular mycorrhizal fungal communities colonizing Sorghum bicolor across sites of contrasting land use history and climate

PLANTS, PEOPLE, PLANET, EarlyView.
Sorghum bicolor is a globally important cereal crop with annual yields exceeding 50 million tons across more than 100 countries and can be grown on marginal lands where conventional agriculture is limited. We examined how eight genetically diverse sorghum genotypes shaped arbuscular mycorrhizal fungal (AMF) community assembly across two contrasting ...
Philip Brailey‐Crane, Sedona M. Spann, Thomas H. Pendergast IV, Ben J. Long, Ashton K. Brinkley, Sarah R. Mondibrown, Nancy C. Johnson, Katrien M. Devos, Jeffrey L. Bennetzen   +8 more
wiley   +1 more source

Development and application of an indirect ELISA and nested PCR for the epidemiological analysis of Klebsiella pneumoniae among pigs in China. [PDF]

Front Microbiol, 2023
Wu Z, Li N, Li Z, Wang J, Liu M, Qi M, Wei S, Wu T, Guo Y, Zhu J, Jiang H, Xue R, Sun C, Feng X, Gu J, Han W, Li F, Lei L.   +17 more
europepmc   +1 more source