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Variants of PCR with more than two primers. I. Nested PCR.
BiomicsNested polymerase chain reaction (nPCR) is a two-step PCR in which the first round uses an outer primer pair to create an amplicon containing binding sites for an inner primer pair. These inner primers, in turn, create a smaller amplicon. A semi-nested PCR variant also exists that employs only one inner primer paired with one of the outer primers in ...
A.R. Sakhabutdinova +4 more
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[Diagnosing achondroplasia by single cell nested-PCR].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics, 2009To research on the reliability of diagnosing achondroplasia (ACH) on single cell level and to provide a basis for preimplantation genetic diagnosis(PGD).The high-frequency mutation region G380R of fibroblast growth factor receptor 3(FGFR3) gene was amplified by nested-PCR with single lymphocyte and single blastomere.
Chang-gao, Zhong +6 more
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Detection and Differentiation of Chlamydiae by Nested PCR
2003Konrad, Sachse, Helmut, Hotzel
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