Single-Tube Nested PCR Using Immobilized Internal Primers
F.G.C. Abath +5 more
doaj +1 more source
Comparison of Immunomagnetic Bead Separation-Immunosensor Detection and Nested-PCR Methods for Detecting Mycobacterium avium Subspecies paratuberculosis in Cattle Feces. [PDF]
Khosravi M +5 more
europepmc +1 more source
Ips sexdentatus is expanding across southern Europe, attacking multiple Pinus species and showing the ability to colonize trees at early stages of decline. Genetic analysis of 668 individuals revealed 106 haplotypes, with Iberian populations as diversity hotspots, reflecting long‐term refugial persistence alongside more recent demographic expansion ...
Dimitrios N. Avtzis +4 more
wiley +1 more source
Evaluation of Two Multiplexed qPCR Assays for Malaria Detection and Speciation: A Comparative Study With Nested PCR and Microscopy. [PDF]
Muyidi AA +11 more
europepmc +1 more source
Brain viral reservoirs were undetectable in neonatal SIV during the first 3 days post infection; antiretroviral therapy (ART) at 3 dpi prevented brain reservoir seeding and normalized metabolic function, highlighting the necessity of very early treatment to protect the developing CNS.
Li Ma +12 more
wiley +1 more source
Specific Primers and Nested PCR Find <i>Trichophyton rubrum</i> Missed by Culture of Ground Toenails from Onychomycosis in Podiatric Patients in Eastern Australia. [PDF]
Santosh AC, Grando D, Lawrie AC.
europepmc +1 more source
Abstract Background and Purpose Acute respiratory distress syndrome (ARDS) and subsequent pulmonary fibrosis are associated with high mortality and limited treatment options. Periostin (POSTN) is a profibrotic mediator predicted to be regulated by microRNA‐19a‐3p (miR‐19a‐3p), but the relevance of this axis in ARDS‐associated pulmonary fibrosis remains
Weilun Liu +14 more
wiley +1 more source
Primary and Nested PCR Amplification of B1 Gene to Confirm Seropositivity of Toxoplasmosis Among Cancer Patients in Sri Lanka. [PDF]
Weerasooriya GPC +2 more
europepmc +1 more source
MEN1 Deficiency Drives Lung Cancer Progression via Activation of MMP10‐Mediated Angiogenesis
MEN1 deficiency promotes JunD‐mediated upregulation of MMP10 expression, which cleaves pro‐HB‐EGF and activates endothelial EGFR. This triggers the PI3K/Akt and MEK/ERK signaling pathways, thereby driving angiogenesis and tumor progression. MMP10 inhibitor treatment blocks this cleavage, suppresses EGFR signaling, and thereby delays malignant ...
Chengyu Wei +17 more
wiley +1 more source
One-Tube Nested PCR Coupled with CRISPR-Cas12a for Ultrasensitive Nucleic Acid Testing. [PDF]
He Y, Peng Y, Tong Y.
europepmc +1 more source

