Results 41 to 50 of about 97,206 (312)

Analysis, comparison, and significance of nested polymerase chain reaction with conventional methods for the diagnosis of pulmonary and extrapulmonary tuberculosis among the rural population of North India

open access: yesCHRISMED Journal of Health and Research, 2019
Introduction: Tuberculosis (TB) is one of the most common infectious diseases among the Indian population, especially in rural scenario. It affects young people in majority. Living at slum areas and poverty are predisposing etiological factors.
Amit Singh, Seema Dayal
doaj   +1 more source

Single genome sequencing of near full-length HIV-1 RNA using a limiting dilution approach [PDF]

open access: yes, 2019
Sequencing very long stretches of the HIV-1 genome can advance studies on virus evolution and in vivo recombination but remains technically challenging.
Demecheleer, Els   +7 more
core   +1 more source

An unusual presentation of a case of human psittacosis [PDF]

open access: yes, 2018
Background: Chlamydia psittaci is a gram-negative, obligate intracellular organism. Birds are the main reservoir, but also non-avian domestic animals and humans can be infected.
Boelens, Jerina   +7 more
core   +3 more sources

Quantitation of Toxoplasma gondii DNA in a competitive nested polymerase chain reaction [PDF]

open access: yesJournal of Clinical Pathology, 1999
To quantify Toxoplasma gondii DNA using a specially constructed artificial template as competitor in a nested polymerase chain reaction (PCR).The diagnostic assay was a nested PCR employing four primers that amplify part of the single copy gene for the P30 major surface antigen in T gondii.
P Y, Lee   +3 more
openaire   +2 more sources

Identification of human papillomavirus DNA in cutaneous lesions of Cowden syndrome [PDF]

open access: yes, 2003
Background: Cowden syndrome (CS) or multiple hamartoma syndrome is a cancer-associated genodermatosis inherited in an autosomal dominant pattern. One of the diagnostic criteria is facial papules which are felt to be trichilemmomas, benign hair follicle ...
Burgdorf, Walter H. C.   +4 more
core   +1 more source

Rapid Detection of Borrelia burgdorferi Strains by Nested Polymerase Chain Reaction

open access: yesPakistan Journal of Biological Sciences, 2009
This study was carried out to evaluate the nested PCR for specific detection of different strains of B. burgdorferi. Five strains of B. burgdorferi including ACA-1, B-31, 2B45, 3B45, 7B49 obtained from different countries were used in this study. The strains of B. hermsii, Escherichia. coli and T. pallidum were also included as control strains.
R, Sorouri   +3 more
openaire   +2 more sources

Identification of the orphan gene Prod 1 in basal and other salamander families. [PDF]

open access: yes, 2015
The urodele amphibians (salamanders) are the only adult tetrapods able to regenerate the limb. It is unclear if this is an ancestral property that is retained in salamanders but lost in other tetrapods or if it evolved in salamanders.
Brockes, JP   +8 more
core   +1 more source

Genetic Evidence of Dobrava Virus in Apodemus agrarius in Hungary

open access: yesEmerging Infectious Diseases, 1999
Using nested polymerase chain reaction, we sequenced Dobrava virus (DOB) from the rodent Apodemus agrarius in Hungary. The samples we isolated group with DOB samples previously isolated from A. flavicollis. This grouping may indicate host switching.
Jerrold J. Scharninghausen   +4 more
doaj   +1 more source

Molecular Exploration of the First-Century Tomb of the Shroud in Akeldama, Jerusalem [PDF]

open access: yes, 2009
The Tomb of the Shroud is a first-century C. E. tomb discovered in Akeldama, Jerusalem, Israel that had been illegally entered and looted. The investigation of this tomb by an interdisciplinary team of researchers began in 2000.
Donoghue, HD   +8 more
core   +5 more sources

Human Cytomegalovirus: detection of congenital and perinatal infection in Argentina [PDF]

open access: yes, 2004
BACKGROUND: Human cytomegalovirus (CMV) is one of the most commonly found agents of congenital infections. Primary maternal infection is associated with risk of symptomatic congenital diseases, and high morbidity is frequently associated with very low ...
Alicia Alonso   +25 more
core   +3 more sources

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