Results 51 to 60 of about 494,637 (215)
Engineering DNA-Templated Nonribosomal Peptide Synthesis [PDF]
Cell Chemical Biology, 2020 Abstract Nanocontainers or macromolecular scaffolds for artificial biocatalytic cascades facilitate sequential enzyme reactions but diffusive escape of intermediates limits rate enhancement. Nonribosomal peptide synthetases (NRPS) naturally form gigantic assembly lines and prevent escape by covalently tethering intermediates.
Hsin-Mei Huang +2 more
openaire +2 more sources
The role of the phosphopantetheinyltransferase enzyme, PswP, in the biosynthesis of antimicrobial secondary metabolites by Serratia marcescens Db10 [PDF]
, 2014 Phosphopantetheinyl transferase (PPTase) enzymes fulfil essential roles in primary and secondary metabolism in prokaryotes, archaea and eukaryotes. PPTase enzymes catalyse the essential modification of the carrier protein domain of fatty acid synthases ...
Gerc, Amy J. +2 more
core +1 more source
Molecular Plant-Microbe Interactions, 2006 Cyclic lipopeptides (CLPs) are versatile molecules produced by a variety of bacterial genera, including plant-associated Pseudomonas spp. CLPs are composed of a fatty acid tail linked to a short oligopeptide, which is cyclized to form a lactone ring ...
Jos M. Raaijmakers +2 more
doaj +1 more source
Adenylation Domain Engineering Enables Biosynthesis of an Improved Polymyxin Antibiotic
Angewandte Chemie, EarlyView.To enable incorporation of l‐2‐aminodecanoic acid into polymyxin by fermentation, a single Trp‐to‐Ala substitution is rationally introduced into the adenylation‐domain residue of its nonribosomal peptide synthetase (NRPS) and efficiently switches substrate selectivity. The engineered strain produces biologically active Ada‐polymyxin, demonstrating that
Maximilian Müll +7 more
wiley +2 more sources
Characterization of cereulide synthetase, a toxin-producing macromolecular machine.
PLoS ONE, 2015 Cereulide synthetase is a two-protein nonribosomal peptide synthetase system that produces a potent emetic toxin in virulent strains of Bacillus cereus.
Diego A Alonzo +2 more
doaj +1 more source
Rapid Peptide Cyclization Inspired by the Modular Logic of Nonribosomal Peptide Synthetases. [PDF]
J Am Chem SocNonribosomal cyclic peptides (NRcPs) are structurally complex natural products and a vital pool of therapeutics, particularly antibiotics. Their structural diversity arises from the ability of the multidomain enzyme assembly lines, nonribosomal peptide ...
Ding Y +12 more
europepmc +3 more sources
ANTIFOULING ACTIVITY OF BACTERIA ASSOCIATED WITH SOFT CORAL Sarcophyton sp. AGAINST MARINE BIOFILMFORMING BACTERIA [PDF]
, 2006 Marine bacteria associated with soft coral Sarcophyton sp collected from vicinity of Peucang island, Ujung Kulon, West Java, were successfully screened for antifouling activity against marine biofilm-forming bacteria isolated from the surrounding ...
Radjasa, Ocky Karna, Sabdono, Agus
core +1 more source
MicrobiologyOpen, 2016 Kalimantacin is an antimicrobial compound with strong antistaphylococcal activity that is produced by a hybrid trans‐acyltransferase polyketide synthase/nonribosomal peptide synthetase system in Pseudomonas fluorescens BCCM_ID9359.
Birgit Uytterhoeven +6 more
doaj +1 more source
Piecing together nonribosomal peptide synthesis
Current Opinion in Structural Biology, 2018 Nonribosomal peptide synthetases (NRPSs) produce peptide products with wide-ranging biological activities. NRPSs are macromolecular machines with modular assembly-line logic, a complex catalytic cycle, moving parts and multiple active sites. They are organized into repeating sets of domains, called modules.
Reimer, Janice M. +3 more
openaire +3 more sources
Frontiers in Microbiology, 2015 Paenibacillus polymyxa is a common soil bacterium with broad range of practical applications. An important group of secondary metabolites in P. polymyxa are nonribosomal peptide and polyketide derived metabolites (NRP/PK).
Salme eTimmusk
doaj +1 more source