Dereplication and de novo sequencing of nonribosomal peptides. [PDF]
Nat Methods, 2009 Nonribosomal peptides (NRPs) are of great pharmacological importance, but there is currently no technology for high-throughput NRP 'dereplication' and sequencing. We used multistage mass spectrometry followed by spectral alignment algorithms for sequencing of cyclic NRPs. We also developed an algorithm for comparative NRP dereplication that establishes
Ng J +8 more
europepmc +4 more sources
Diversity of monomers in nonribosomal peptides: towards the prediction of origin and biological activity. [PDF]
J Bacteriol, 2010 Nonribosomal peptides (NRPs) are molecules produced by microorganisms that have a broad spectrum of biological activities and pharmaceutical applications (e.g., antibiotic, immunomodulating, and antitumor activities). One particularity of the NRPs is the
Caboche S +4 more
europepmc +2 more sources
A Journey into the Blue: Current Knowledge and Emerging Insights into Marine-Derived Peptaibols [PDF]
Marine DrugsPeptaibols represent a large family of membrane-active, linear fungal peptides, with variable lengths from 5 to 21 α–amino acid residues. As products of nonribosomal peptide synthetase (NRPS) biosynthetic machinery, they encompass several non ...
Claudia Finamore +4 more
doaj +2 more sources
Paenitracins, a novel family of bacitracin-type nonribosomal peptide antibiotics produced by plant-associated Paenibacillus species [PDF]
mSystemsThe growing threat of antimicrobial resistance necessitates the discovery of novel antibiotics with activity against drug-resistant pathogens. Members of the genus Paenibacillus are a rich source of nonribosomal peptides (NRPs), including well-known ...
Nataliia V. Machushynets +9 more
doaj +2 more sources
CycloBranch: De Novo Sequencing of Nonribosomal Peptides from Accurate Product Ion Mass Spectra
Journal of the American Society for Mass Spectrometry, 2015 Vladimir Havlicek +2 more
exaly +2 more sources
Metagenomic domain substitution for the high-throughput modification of nonribosomal peptides
Nature Chemical Biology, 2023 The modular nature of nonribosomal peptide biosynthesis has driven efforts to generate peptide analogs by substituting amino acid-specifying domains within nonribosomal peptide synthetase (NRPS) enzymes. Rational NRPS engineering has increasingly focused
Sarah R. Messenger +6 more
semanticscholar +1 more source
Nonribosomal Peptide Synthetases in Animals
Genes, 2023 Nonribosomal peptide synthetases (NRPSs) are a class of cytosolic enzymes that synthesize a range of bio-active secondary metabolites including antibiotics and siderophores. They are widespread among both prokaryotes and eukaryotes but are considered rare among animals.
Wouter Suring +5 more
openaire +3 more sources
Identification of a Novel Biosynthetic Gene Cluster in Aspergillus niger Using Comparative Genomics
Journal of Fungi, 2021 Previously, DNA microarrays analysis showed that, in co-culture with Bacillus subtilis, a biosynthetic gene cluster anchored with a nonribosomal peptides synthetase of Aspergillus niger is downregulated.
Gregory Evdokias +7 more
doaj +1 more source
A Nonribosomal System of Peptide Biosynthesis [PDF]
European Journal of Biochemistry, 1996 This review covers peptide structures originating from the concerted action of enzyme systems without the direct participation of nucleic acids. Biosynthesis proceeds by formation of linear peptidyl intermediates which may be enzymatically modified as well as transformed into specific cyclic structures.
H, Kleinkauf, H, Von Döhren
openaire +2 more sources
Pentaminomycins F and G, Nonribosomal Peptides Containing 2-Pyridylalanine.
Journal of Natural Products, 2021 Pentaminomycins F-H (1-3), a group of three new hydroxyarginine-containing cyclic pentapeptides, were isolated from cultures of a Streptomyces cacaoi subsp. cacaoi strain along with the known pentaminomycins A-E.
Daniel Carretero-Molina +9 more
semanticscholar +1 more source