Results 91 to 100 of about 9,796 (161)

A novel and universal method for microRNA RT-qPCR data normalization [PDF]

, 2009
Gene expression analysis of microRNA molecules is becoming increasingly important. In this study we assess the use of the mean expression value of all expressed microRNAs in a given sample as a normalization factor for microRNA real-time quantitative PCR
De Weer, An, Mestdagh, Pieter, Muth, Daniel, Speleman, Franki, Van Vlierberghe, Pieter, Vandesompele, Jo, Westermann, Frank   +6 more
core   +2 more sources

Evaluation of reference genes for reverse transcription-quantitative PCR assays in organs of zebrafish exposed to glyphosate-based herbicide, Roundup

Animal, 2018
Roundup is a glyphosate-based herbicide (GBH) widely used in agriculture and may cause toxic effects in non-target organisms. Model organisms, as zebrafish, and analysis of gene expression by reverse transcription-quantitative PCR (RT-qPCR) could be used
M.L. Jaramillo, A.G. Pereira, C.E. Davico, L. Nezzi, D. Ammar, Y.M.R. Müller, E.M. Nazari   +6 more
doaj   +1 more source

Identification and Evaluation of qRT-PCR Reference Genes in Melanaphis sacchari

Insects
Appropriate reference genes must be selected for accurate qRT-PCR data to conduct a thorough gene expression analysis in the sorghum aphid (Melanaphis sacchari, Hemiptera, Aphididae). This approach will establish a foundation for gene expression analysis
Kunliang Zou, Tonghan Wang, Minghui Guan, Yang Liu, Jieqin Li, Yanlong Liu, Junli Du, Degong Wu   +7 more
doaj   +1 more source

Reference genes for gene expression studies targeting sugarcane infected with Sugarcane mosaic virus (SCMV)

BMC Research Notes, 2019
Objective The selection of reference genes in sugarcane under Sugarcane mosaic virus (SCMV) infection has not been reported and is indispensable to get reliable reverse transcription quantitative PCR (RT-qPCR) results for validation of transcriptome ...
Marcel Fernando da Silva, Marcos Cesar Gonçalves, Michael dos Santos Brito, Paula Macedo Nóbile, Larissa Mara de Andrade, Cibele Nataliane Medeiros, Silvana Creste, Luciana Rossini Pinto   +7 more
doaj   +1 more source

Selection and validation of reference genes for quantitative RT-PCR expression studies of the non-model crop Musa [PDF]

, 2014
Gene expression analysis by reverse transcriptase real-time or quantitative polymerase chain reaction (RT-qPCR) is becoming widely used for nonmodel plant species.
Henry, I., Krauss, A., Podevin, N., Remy, S., Swennen, Rony L.   +4 more
core   +1 more source

Reference Genes Selection and Normalization of Oxidative Stress Responsive Genes upon Different Temperature Stress Conditions in Hypericum perforatum L [PDF]

, 2017
Reverse transcription-quantitative real-time PCR (RT-qPCR) is a widely used technique for gene expression analysis. The reliability of this method depends largely on the suitable selection of stable reference genes for accurate data normalization ...
Arnholdt-Schmitt, Birgit, Cardoso, Hélia, Ragonezi, Carla, Velada, Isabel   +3 more
core  

Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions. [PDF]

, 2014
Quantitative real-time polymerase chain reaction (RT-qPCR) is a powerful tool used to measure gene expression. However, because of its high sensitivity, the method is strongly influenced by the quality and concentration of the template cDNA and by the ...
BORÉM, A., EMYGDIO, B. M., FARIAS, J. R. B., MARCELINO-GUIMARÃES, F. C., NAKAYAMA, T. J., NEPOMUCENO, A. L., NEUMAIER, N., OLIVEIRA, A. C. B. de, OLIVEIRA, M. C. N. de, RODRIGUES, F. A.   +9 more
core   +2 more sources

Selection of reference genes for quantitative RT-PCR (RT-qPCR) analysis of rat tissues under physiological and toxicological conditions [PDF]

, 2015
In biological research the analysis of gene expression levels in cells and tissues can be a powerful tool to gain insights into biological processes. For this, quantitative RT-PCR (RT-qPCR) is a popular method that often involve the use of constitutively
Hadrup, Niels, Hass, Ulla, Letting, Heidi, Svingen, Terje, Vinggaard, Anne Marie   +4 more
core   +3 more sources

Selection of housekeeping genes for gene expression studies in larvae from flatfish using real-time PCR [PDF]

, 2008
Flatfish metamorphosis involves major physiological and morphological changes. Due to its importance in aquaculture and as a model for developmental studies, some gene expression studies have focused on the understanding of this process using ...
Infante, C.
core   +1 more source

Seleção de genes endógenos para análise de qRT-PCR em sementes de café. [PDF]

, 2014
A técnica quantitativa de PCR em tempo real, qRT-PCR, permite analisar a expressão de genes alvo em diferentes condições experimentais. No entanto, a análise precisa dos resultados gerados pela qRT-PCR está diretamente relacionada ao gene normalizador ...
CARDOSO, D. C., MALUF, M. P., PADILHA, L., SCHENK, J. C. M.   +3 more
core