Results 91 to 100 of about 9,815 (163)

Differential changes in anatomical, molecular and functional determinants of intestinal glucose absorption during murine pregnancy

Physiological Reports, Volume 13, Issue 15, August 2025.
Abstract Pregnancy demands increased food intake and nutrient delivery to support the growing conceptus. The timing and regional specificity of adaptations in small intestinal (SI) anatomy and transport, and their functional consequences, remain unclear. We therefore assessed anatomy and expression of macronutrient transporters in the duodenum, jejunum,
T. Sebastian Overduin, Georgia S. Clarke, Hui Li, Richard L. Young, Kathryn L. Gatford, Amanda J. Page   +5 more
wiley   +1 more source

Using RNA-Seq to Identify Reference Genes of the Transition from Brown to White Adipose Tissue in Goats

Animals, 2020
Brown adipose tissues have unique non-shivering thermogenesis functions, can be found in newborn ruminate animals, and then are gradually replaced by white adipose tissues in adulthood.
Linjie Wang, Xingyue Chen, Tianzeng Song, Xujia Zhang, Siyuan Zhan, Jiaxue Cao, Tao Zhong, Jiazhong Guo, Li Li, Hongping Zhang, Yan Wang   +10 more
doaj   +1 more source

Construction of an adult barnacle () cDNA library and selection of reference genes for quantitative RT-PCR studies [PDF]

, 2009
De Gregoris TB, Borra M, Biffali E, et al. Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection of reference genes for quantitative RT-PCR studies. BMC Molecular Biology.
Tristano Bacchetti De Gregoris, Marco Borra, Elio Biffali, Thomas Bekel, J Grant Burgess, Richard R Kirby, Anthony S Clare, Bacchetti De Gregoris, Tristano   +7 more
core   +2 more sources

Validation of housekeeping genes for quantitative real-time PCR in in-vivo and in-vitro models of cerebral ischaemia [PDF]

, 2009
Studies of gene expression in experimental cerebral ischaemia models can contribute to understanding the pathophysiology of brain ischaemia and to identifying prognostic markers and potential therapeutic targets.
Carme Gubern, Olivia Hurtado, Rocío Rodríguez, Jesús R Morales, Víctor G Romera, María A Moro, Ignacio Lizasoain, Joaquín Serena, Judith Mallolas   +8 more
core   +1 more source

Evaluation of reference genes for reverse transcription-quantitative PCR assays in organs of zebrafish exposed to glyphosate-based herbicide, Roundup

Animal, 2018
Roundup is a glyphosate-based herbicide (GBH) widely used in agriculture and may cause toxic effects in non-target organisms. Model organisms, as zebrafish, and analysis of gene expression by reverse transcription-quantitative PCR (RT-qPCR) could be used
M.L. Jaramillo, A.G. Pereira, C.E. Davico, L. Nezzi, D. Ammar, Y.M.R. Müller, E.M. Nazari   +6 more
doaj   +1 more source

Identification and Evaluation of qRT-PCR Reference Genes in Melanaphis sacchari

Insects
Appropriate reference genes must be selected for accurate qRT-PCR data to conduct a thorough gene expression analysis in the sorghum aphid (Melanaphis sacchari, Hemiptera, Aphididae). This approach will establish a foundation for gene expression analysis
Kunliang Zou, Tonghan Wang, Minghui Guan, Yang Liu, Jieqin Li, Yanlong Liu, Junli Du, Degong Wu   +7 more
doaj   +1 more source

Reference genes for gene expression studies targeting sugarcane infected with Sugarcane mosaic virus (SCMV)

BMC Research Notes, 2019
Objective The selection of reference genes in sugarcane under Sugarcane mosaic virus (SCMV) infection has not been reported and is indispensable to get reliable reverse transcription quantitative PCR (RT-qPCR) results for validation of transcriptome ...
Marcel Fernando da Silva, Marcos Cesar Gonçalves, Michael dos Santos Brito, Paula Macedo Nóbile, Larissa Mara de Andrade, Cibele Nataliane Medeiros, Silvana Creste, Luciana Rossini Pinto   +7 more
doaj   +1 more source

Gene expression markers of tendon fibroblasts in normal and diseased tissue compared to monolayer and three dimensional culture systems [PDF]

, 2009
Background: There is a paucity of data regarding molecular markers that identify the phenotype of the tendon cell. This study aims to quantify gene expression markers that distinguish between tendon fibroblasts and other mesenchymal cells which may be ...
Sarah E Taylor, Anne Vaughan-Thomas, Dylan N Clements, Gina Pinchbeck, Lisa C Macrory, Roger KW Smith, Peter D Clegg, A McCormick, BA Dowling, Y Kasashima, JC Patterson-Kane, JH Wang, SJ Dyson, LE Richardson, G Riley, DL Butler, QW Wang, A Barbero, GP Riley, PD Clegg, LA Dahlgren, HA Eriksen, TJ Molloy, P Sharma, GP Riley, Y Ezura, RK Smith, R Chiquet-Ehrismann, K Halasz, D Docheva, C Shukunami, G Zhang, ND Murchison, R Schweitzer, Y Bi, K Ninomiya, J Garvin, C Reno, DN Clements, J Vandesompele, CL Andersen, L de Palma, D Mehr, KG Vogel, TA Jarvinen, Y Asou, H Aslan, M de Mos, H Alfredson, D Ireland, N Sawaguchi, L Yao, L Zhang, Z Kapacee, LJ Maccoux   +54 more
core   +2 more sources

Validating reference genes using minimally transformed qpcr data: findings in human cortex and outcomes in schizophrenia [PDF]

, 2016
BACKGROUND: It is common practice, when using quantitative real time polymerase chain reaction (qPCR), to normalise levels of mRNA to reference gene mRNA which, by definition, should not vary between tissue, with any disease aetiology or after drug ...
Brian Dean, Elizabeth Scarr, Madhara Udawela   +2 more
core   +4 more sources

Reference gene screening of Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans for quantitative real-time PCR studies [PDF]

, 2019
Real-time quantitative PCR studies largely depend on reference genes for the normalization of gene expression. Stable reference genes should be accurately selected in order to obtain reliable results.
Martel, An, Pasmans, Frank, Verbrugghe, Elin   +2 more
core   +1 more source