Results 11 to 20 of about 17,579 (227)

Identification of novel neutralizing determinants for protection against HCV

Hepatology, EarlyView., 2022
Identification of novel neutralizing determinants for protection against hepatitis C virus. Abstract Background and Aims HCV evasion of neutralizing antibodies (nAb) results in viral persistence and poses challenges to the development of an urgently needed vaccine.
Garazi P. Alzua, Anne F. Pihl, Anna Offersgaard, Rodrigo Velázquez‐Moctezuma, Carlos R. Duarte Hernandez, Elias H. Augestad, Ulrik Fahnøe, Christian K. Mathiesen, Henrik Krarup, Mansun Law, Jannick Prentoe, Jens Bukh, Judith M. Gottwein   +12 more
wiley   +1 more source

Genotyping atypical porcine pestivirus using NS5a

Infection, Genetics and Evolution, 2021
Atypical porcine pestivirus (APPV) is an emerging virus discovered in 2014 and it can cause congenital tremors in pigs. Molecular epidemiology serves as an essential tool in monitoring and controlling the disease. Virus epidemiology mainly relies on genome sequencing and phylogenetic characterization.
Fangfeng, Yuan, Leyi, Wang
openaire   +2 more sources

Synergistic Activity of Combined NS5A Inhibitors [PDF]

Antimicrobial Agents and Chemotherapy, 2016
ABSTRACT Daclatasvir (DCV) is a first-in-class hepatitis C virus (HCV) nonstructural 5A replication complex inhibitor (NS5A RCI) that is clinically effective in interferon-free combinations with direct-acting antivirals (DAAs) targeting alternate HCV proteins.
Donald R, O'Boyle, Peter T, Nower, Min, Gao, Robert, Fridell, Chunfu, Wang, Piyasena, Hewawasam, Omar, Lopez, Yong, Tu, Nicholas A, Meanwell, Makonen, Belema, Susan B, Roberts, Mark, Cockett, Jin-Hua, Sun   +12 more
openaire   +2 more sources

Identification of Hepatitis C Virus NS5A Inhibitors [PDF]

Journal of Virology, 2010
ABSTRACT Using a cell-based replicon screen, we identified a class of compounds with a thiazolidinone core structure as inhibitors of hepatitis C virus (HCV) replication. The concentration of one such compound, BMS-824, that resulted in a 50% inhibition of HCV replicon replication was ∼5 nM, with a therapeutic index of >10,000. The
Julie A, Lemm, Donald, O'Boyle, Mengping, Liu, Peter T, Nower, Richard, Colonno, Milind S, Deshpande, Lawrence B, Snyder, Scott W, Martin, Denis R, St Laurent, Michael H, Serrano-Wu, Jeffrey L, Romine, Nicholas A, Meanwell, Min, Gao   +12 more
openaire   +2 more sources

Hepatitis C virus NS5A targets the nucleosome assembly protein NAP1L1 to control the innate cellular response [PDF]

, 2017
Hepatitis C virus (HCV) is a single-stranded positive-sense RNA hepatotropic virus. Despite cellular defenses, HCV is able to replicate in hepatocytes and to establish a chronic infection that could lead to severe complications and hepatocellular ...
Cesarec, Mia, Da Silva Filipe, Ana, Licastro, Danilo, Marcello, Alessandro, McLauchlan, John, Çevik, Recep Emrah   +5 more
core   +1 more source

HCV NS5A dimer interface residues regulate HCV replication by controlling its self-interaction, hyperphosphorylation, subcellular localization and interaction with cyclophilin A.

PLoS Pathogens, 2018
The HCV NS5A protein plays multiple roles during viral replication, including viral genome replication and virus particle assembly. The crystal structures of the NS5A N-terminal domain indicated the potential existence of the NS5A dimers formed via at ...
Saravanabalaji Shanmugam, Alyssa K Nichols, Dhanaranjani Saravanabalaji, Christoph Welsch, MinKyung Yi   +4 more
doaj   +1 more source

Direct binding of ledipasvir to HCV NS5A: mechanism of resistance to an HCV antiviral agent.

PLoS ONE, 2015
Ledipasvir, a direct acting antiviral agent (DAA) targeting the Hepatitis C Virus NS5A protein, exhibits picomolar activity in replicon cells. While its mechanism of action is unclear, mutations that confer resistance to ledipasvir in HCV replicon cells ...
Hyock Joo Kwon, Weimei Xing, Katie Chan, Anita Niedziela-Majka, Katherine M Brendza, Thorsten Kirschberg, Darryl Kato, John O Link, Guofeng Cheng, Xiaohong Liu, Roman Sakowicz   +10 more
doaj   +1 more source

Rab18 binds to classical swine fever virus NS5A and mediates viral replication and assembly in swine umbilical vein endothelial cells

Virulence, 2020
Classical swine fever virus (CSFV), a positive-sense RNA virus, hijacks cell host proteins for its own replication. Rab18, a small Rab GTPase, regulates intracellular membrane-trafficking events between various compartments in cells and is involved in ...
Liang Zhang, Di Zhao, Mingxing Jin, Mengzhao Song, Shanchuan Liu, Kangkang Guo, Yanming Zhang   +6 more
doaj   +1 more source

The effects of NS5A inhibitors on NS5A phosphorylation, polyprotein processing and localization

Journal of General Virology, 2011
Hepatitis C virus (HCV) non-structural protein 5A (NS5A) is a multi-functional protein that is expressed in basally phosphorylated (p56) and in hyperphosphorylated (p58) forms. NS5A phosphorylation has been implicated in regulating multiple aspects of HCV replication. We recently reported the identification of a class of compounds that potently inhibit
Dike, Qiu, Julie A, Lemm, Donald R, O'Boyle, Jin-Hua, Sun, Peter T, Nower, Van, Nguyen, Lawrence G, Hamann, Lawrence B, Snyder, Daniel H, Deon, Edward, Ruediger, Nicholas A, Meanwell, Makonen, Belema, Min, Gao, Robert A, Fridell   +13 more
openaire   +2 more sources

Visualization of positive and negative sense viral RNA for probing the mechanism of direct-acting antivirals against hepatitis C virus [PDF]

, 2019
RNA viruses are highly successful pathogens and are the causative agents for many important diseases. To fully understand the replication of these viruses it is necessary to address the roles of both positive-strand RNA ((+)RNA) and negative-strand RNA ((
Bassit, Leda C, Huber, Andrew D, Ji, Juan, Lan, Shuiyun, Liu, Dandan, Michailidis, Eleftherios, Ndongwe, Tanyaradzwa P, Puray-Chavez, Maritza N, Ralston, Robert, Rice, Charles M, Saeed, Mohsan, Sarafianos, Stefan G, Schinazi, Raymond F, Tedbury, Philip R   +13 more
core   +2 more sources