Results 41 to 50 of about 12,817 (217)

Characterization of ApoE variants and NS5A mutants.

, 2023
(A) ApoE-NS5A colocalization in cells replicating a full-length HCV genome. Huh7-Lunet/ApoEmT2 cells were electroporated with in vitro transcripts of the HCV genome Jc1.
Karl Rohr (41937), Uta Haselmann (12969434), Janis Meyer (16705902), Vibor Laketa (12969446), Ralf Bartenschlager (32666), Minh-Tu Pham (16705896), Ji-Young Lee (113856), Roman Thielemann (16705899), Charlotta Funaya (84151), Christian Ritter (3447533)   +9 more
core   +1 more source

Fast Hepatitis C Virus RNA Elimination and NS5A Redistribution by NS5A Inhibitors Studied by a Multiplex Assay Approach [PDF]

Antimicrobial Agents and Chemotherapy, 2015
ABSTRACT While earlier therapeutic strategies for the treatment of hepatitis C virus (HCV) infection relied exclusively on interferon (IFN) and ribavirin (RBV), four direct-acting antiviral agents (DAAs) have now been approved, aiming for an interferon-free strategy with a short treatment duration and fewer side effects.
Dandan, Liu, Juan, Ji, Tanya P, Ndongwe, Eleftherios, Michailidis, Charles M, Rice, Robert, Ralston, Stefan G, Sarafianos   +6 more
openaire   +2 more sources

HCV NS5A protein containing potential ligands for both Src homology 2 and 3 domains enhances autophosphorylation of Src family kinase Fyn in B cells.

PLoS ONE, 2012
Hepatitis C virus (HCV) infects B lymphocytes and induces mixed cryoglobulinemia and B cell non-Hodgkin's lymphoma. The molecular mechanism for the pathogenesis of HCV infection-mediated B cell disorders remains obscure. To identify the possible role for
Kenji Nakashima, Kenji Takeuchi, Kazuyasu Chihara, Tomoko Horiguchi, Xuedong Sun, Lin Deng, Ikuo Shoji, Hak Hotta, Kiyonao Sada   +8 more
doaj   +1 more source

Characterization of purified NS5A.

, 2015
(A) Coomassie staining of purified proteins. Recombinant His-tagged proteins were purified as described in Materials and Methods. 1 μg of NS5A-6HIS and NS5A-Y93H-6HIS were subjected to SDS-PAGE and the protein bands were visualized by Coomassie Blue ...
Katherine M. Brendza (158359), Anita Niedziela-Majka (385638), Weimei Xing (431261), Hyock Joo Kwon (353193), Darryl Kato (719707), John O. Link (719708), Guofeng Cheng (719709), Katie Chan (719705), Thorsten Kirschberg (719706), Xiaohong Liu (50816), Roman Sakowicz (79348)   +10 more
core   +1 more source

Resistance Analysis of a 3-Day Monotherapy Study with Glecaprevir or Pibrentasvir in Patients with Chronic Hepatitis C Virus Genotype 1 Infection

Viruses, 2018
Glecaprevir (an NS3/4A protease inhibitor) and pibrentasvir (an NS5A inhibitor) are potent and pangenotypic hepatitis C virus (HCV) direct-acting antivirals.
Teresa I. Ng, Tami Pilot-Matias, Rakesh Tripathi, Gretja Schnell, Preethi Krishnan, Thomas Reisch, Jill Beyer, Tatyana Dekhtyar, Michelle Irvin, Liangjun Lu, Armen Asatryan, Andrew Campbell, Betty Yao, Sandra Lovell, Federico Mensa, Eric J. Lawitz, Jens Kort, Christine Collins   +17 more
doaj   +1 more source

Drug Resistance Profile and Clinical Features for Hepatitis C Patients Experiencing DAA Failure in Taiwan

Viruses, 2021
About 4% of the population in Taiwan are seropositive for anti-HCV Ab and 70% with HCV RNA. To address this high chronic hepatitis C disease load, Taiwan National Health Insurance started reimbursing genotype-specific DAAs in 2017 and pangenotype DAAs in
Chun-Ming Hong, You-Yu Lin, Chun-Jen Liu, Ya-Yun Lai, Shiou-Hwei Yeh, Hung-Chih Yang, Jia-Horng Kao, Shih-Jer Hsu, Yi-Hsiang Huang, Sheng-Shun Yang, Hsing-Tao Kuo, Pin-Nan Cheng, Ming-Lung Yu, Pei-Jer Chen   +13 more
doaj   +1 more source

Co-precipitation of BMS-Biotin with NS5A and NS5A-Y93H.

, 2015
(A) Genotype 1b, Con1 replicon cells (wild type or harboring the Y93H mutant of NS5A) were treated with 1μM DCV or 25 μM BMS-Biotin and incubated for 24 hr. Cells were harvested and the membrane fraction was solubilized with C12E8.
Katherine M. Brendza (158359), Anita Niedziela-Majka (385638), Weimei Xing (431261), Hyock Joo Kwon (353193), Darryl Kato (719707), John O. Link (719708), Guofeng Cheng (719709), Katie Chan (719705), Thorsten Kirschberg (719706), Xiaohong Liu (50816), Roman Sakowicz (79348)   +10 more
core   +1 more source

The lipid kinase phosphatidylinositol-4 kinase III alpha regulates the phosphorylation status of hepatitis C virus NS5A.

PLoS Pathogens, 2013
The lipid kinase phosphatidylinositol 4-kinase III alpha (PI4KIIIα) is an essential host factor of hepatitis C virus (HCV) replication. PI4KIIIα catalyzes the synthesis of phosphatidylinositol 4-phosphate (PI4P) accumulating in HCV replicating cells due ...
Simon Reiss, Christian Harak, Inés Romero-Brey, Danijela Radujkovic, Rahel Klein, Alessia Ruggieri, Ilka Rebhan, Ralf Bartenschlager, Volker Lohmann   +8 more
doaj   +1 more source

eEF1A Interacts with the NS5A Protein and Inhibits the Growth of Classical Swine Fever Virus

Viruses, 2015
The NS5A protein of classical swine fever virus (CSFV) is involved in the RNA synthesis and viral replication. However, the NS5A-interacting cellular proteins engaged in the CSFV replication are poorly defined.
Su Li, Shuo Feng, Jing-Han Wang, Wen-Rui He, Hua-Yang Qin, Hong Dong, Lian-Feng Li, Shao-Xiong Yu, Yongfeng Li, Hua-Ji Qiu   +9 more
doaj   +1 more source

Domain I of NS5A binds to core.

, 2014
A) NS5A is composed of three domains (Domain I, II, and III) separated by low-complexity sequences (LCSI and LCSII). Domain I of NS5A is composed of a 32-amino-acid N-terminal amphipathic helix (AH), subdomain IA (33–100) and subdomain IB (101–213).
James Baugh (522244), Philippe A. Gallay (351890), Michael D. Bobardt (351884), Katarzyna Gawlik (266244), Udayan Chatterji (238995), Precious J. Lim (522245)   +5 more
core   +1 more source