Results 21 to 30 of about 3,842 (144)

Amino acid substitutions in NSP6 and NSP13 of SARS-CoV-2 contribute to superior virus growth at low temperatures [PDF]

Journal of Virology
In general, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replicates well at 37°C, which is the temperature of the human lower respiratory tract, but it poorly at 30°C‒32°C, which is the temperature of the human upper respiratory tract ...
Yuri Furusawa, Maki Kiso, Ryuta Uraki, Yuko Sakai-Tagawa, Hiroyuki Nagai, Michiko Koga, Yukie Kashima, Masayuki Hojo, Noriko Iwamoto, Kiyoko Iwatsuki-Horimoto, Norio Ohmagari, Yutaka Suzuki, Hiroshi Yotsuyanagi, Peter J. Halfmann, Wataru Kamitani, Seiya Yamayoshi, Yoshihiro Kawaoka   +16 more
doaj   +2 more sources

Z-RNA and the flipside of the SARS Nsp13 helicase [PDF]

, 2022
Abstract We present evidence that the severe acute respiratory syndrome coronavirus (SARS) non-structural protein 13 (Nsp13) modulates the Z-RNA dependent regulated cell death pathway of necroptosis (1). We show that Z-prone sequences (called flipons (2)) exist in coronavirus and provide a signature that enables identification of the animal ...
Alan Herbert, Alex Shein, Maria Poptsova
openaire   +3 more sources

Development of a Fluorescent Assay and Imidazole-Containing Inhibitors by Targeting SARS-CoV-2 Nsp13 Helicase

Molecules
Nsp13, a non-structural protein belonging to the coronavirus family 1B (SF1B) helicase, exhibits 5′–3′ polarity-dependent DNA or RNA unwinding using NTPs.
Fei Jin, Qingqing Zhang
exaly   +3 more sources

2-Phenylquinolines Exhibit Anti-Severe Acute Respiratory Syndrome Coronavirus-2 Activity Through the Nonstructural Protein 13 Helicase Inhibition. [PDF]

ChemMedChem
A SAR study led to the identification of new 2‐phenylquinoline‐based derivatives that exhibit anti‐Severe Acute Respiratory Syndrome Coronavirus‐2 (SARS‐CoV‐2) activity by acting as ATP‐competitive inhibitors of nonstructural protein 13 (nsp13) helicase.
Cernicchi G, Nizi MG, Emmolo R, Persoons L, Laporte M, Jochmans D, Romeo I, Pepe G, Milite C, Campiglia P, Spezzini J, Martelli A, Brogi S, Esposito F, Felicetti T, Massari S, Manfroni G, Sabatini S, Sbardella G, Alcaro S, Neyts J, Corona A, Tramontano E, De Jonghe S, Tabarrini O.   +24 more
europepmc   +2 more sources

The NSP6-L260F substitution in SARS-CoV-2 BQ.1.1 and XBB.1.16 lineages compensates for the reduced viral polymerase activity caused by mutations in NSP13 and NSP14 [PDF]

Journal of Virology
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variants emerged at the end of 2021, and their subvariants are still circulating worldwide.
Yuri Furusawa, Kiyoko Iwatsuki-Horimoto, Seiya Yamayoshi, Yoshihiro Kawaoka   +3 more
doaj   +2 more sources

The Role of ATP in the RNA Translocation Mechanism of SARS-CoV-2 NSP13 Helicase [PDF]

The Journal of Physical Chemistry B, 2021
Abstract The COVID-19 pandemic has demonstrated the need to develop potent and transferable therapeutics to treat coronavirus infections. Numerous antiviral targets are being investigated, but non-structural protein 13 (nsp13) stands out as a highly conserved and yet under studied target. Nsp13 is a superfamily 1 (SF1)
Ryan Weber, Martin McCullagh
openaire   +3 more sources

Discovery of COVID-19 Inhibitors Targeting the SARS-CoV-2 Nsp13 Helicase [PDF]

The Journal of Physical Chemistry Letters, 2020
ABSTRACT The raging COVID-19 pandemic caused by SARS-CoV2 has infected millions of people and killed several hundred thousand patients worldwide. Currently, there are no effective drugs or vaccines available for treating coronavirus infections. In this study, we have focused on the SARS-CoV2 helicase (Nsp13), which is critical for viral
White, Mark Andrew, Lin, Wei, Cheng, Xiaodong   +2 more
openaire   +3 more sources

Force-dependent stimulation of RNA unwinding by SARS-CoV-2 nsp13 helicase [PDF]

Biophysical Journal, 2020
ABSTRACT The superfamily-1 helicase non-structural protein 13 (nsp13) is required for SARS-CoV-2 replication, making it an important antiviral therapeutic target. The mechanism and regulation of nsp13 has not been explored at the single-molecule level.
Keith J. Mickolajczyk, Patrick M.M. Shelton, Michael Grasso, Xiaocong Cao, Sara E. Warrington, Amol Aher, Shixin Liu, Tarun M. Kapoor   +7 more
openaire   +4 more sources

Structural Analysis on the Severe Acute Respiratory Syndrome Coronavirus 2 Non-structural Protein 13 Mutants Revealed Altered Bonding Network With TANK Binding Kinase 1 to Evade Host Immune System

Frontiers in Microbiology, 2021
Mutations in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have made this virus more infectious. Previous studies have confirmed that non-structural protein 13 (NSP13) plays an important role in immune evasion by physically interacting ...
Farooq Rashid, Muhammad Suleman, Abdullah Shah, Emmanuel Enoch Dzakah, Shuyi Chen, Haiying Wang, Shixing Tang, Shixing Tang, Shixing Tang   +8 more
doaj   +1 more source

Mechanism of nucleic acid unwinding by SARS-CoV helicase. [PDF]

PLoS ONE, 2012
The non-structural protein 13 (nsp13) of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) is a helicase that separates double-stranded RNA (dsRNA) or DNA (dsDNA) with a 5' → 3' polarity, using the energy of nucleotide hydrolysis.
Adeyemi O Adedeji, Bruno Marchand, Aartjan J W Te Velthuis, Eric J Snijder, Susan Weiss, Robert L Eoff, Kamalendra Singh, Stefan G Sarafianos   +7 more
doaj   +1 more source