Results 41 to 50 of about 332 (132)

SARS‐CoV‐2 targets mitochondria, exacerbating COVID‐19 pneumonia

The Journal of Physiology, EarlyView.
Abstract figure legend Following entry into airway epithelial cells (AECs), SARS‐CoV‐2 releases its single‐stranded RNA into the cytoplasm, where it is translated into viral proteins. Several of these viral proteins localize to mitochondria and interact with key mitochondrial components.
Danchen Wu, Asish Dasgupta, Jaskaran Singh Hora, Kuang‐Hueih Chen, Arinjay Banerjee, Stephen L. Archer   +5 more
wiley   +1 more source

Murine hepatitis virus nsp4 N258T mutants are not temperature-sensitive

Virology, 2013
Coronavirus replicase nsp4 is critical for virus-induced membrane modifications. An nsp4 mutant (N258T) of murine hepatitis virus (MHV) has been reported to be temperature-sensitive (ts) and to alter membrane targeting. We engineered and recovered all four possible codon variants of N258T in the cloned MHV-A59 background.
Beachboard, Dia C., Lu, Xiaotao, Baker, Susan C., Denison, Mark R.   +3 more
openaire   +2 more sources

NSP4 as adjuvant for immunogenicity and design of effective therapeutic HPV16 E6/E7/L1 DNA vaccine in tumor-bearing and healthy C57BL/6 mice

BMC Research Notes, 2023
Introduction In humans, approximately 5% of all cancers are attributable to HPV infection. Prophylactic vaccines can inhibit viral migration and persistence. However, further studies are still required to develop such treatments. To achieve this goal, we
Sahar Sadr-Momtaz, Maryam Aftabi, Emad Behboudi, Malihe Naderi, Anahita Hashemzadeh-Omran, Abdolvahab Moradi   +5 more
doaj   +1 more source

The C117D oxidation mimic reveals the monomeric structure of SARS‐CoV‐2 main protease

Protein Science, Volume 35, Issue 9, September 2026.
Abstract The SARS‐CoV‐2 main protease (Mpro) is essential for viral replication and functions as a homodimer, with dimerization being critical for catalytic activity. Mpro contains an unusually high number of cysteine residues. Among these, C117 and the catalytic nucleophile C145 can form a reversible disulfide bond under oxidative conditions.
Sam Andress, Matthew J. McLeod, Todd Holyoak   +2 more
wiley   +1 more source

The rotavirus nonstructural glycoprotein NSP4 possesses membrane destabilization activity [PDF]

Journal of Virology, 1996
During a unique morphogenetic process, rotaviruses obtain a transient membrane envelope when newly synthesized subviral particles bud into the endoplasmic reticulum (ER). As rotavirus particles mature, they lose their transient membrane and a layer of the glycoprotein VP7 forms the virion outer capsid shell.
P, Tian, J M, Ball, C Q, Zeng, M K, Estes   +3 more
openaire   +2 more sources

Identification of Putative Novel Rotavirus H VP7, VP4, VP6 and NSP4 Genotypes in Pigs

Viruses, 2023
Rotavirus H (RVH) has been detected in humans, pigs and bats. Recently, RVH infections were reported in different porcine farms worldwide, suggesting epidemiological relevance.
Elena Ferrari, Greta Vignola, Cristina Bertasio, Chiara Chiapponi, Giovanni Loris Alborali, Vito Martella, Maria Beatrice Boniotti   +6 more
doaj   +1 more source

Recent Advances in Fluorescent Probes for Imaging Enzyme Activity in NETosis

ChemBioChem, Volume 27, Issue 15, 14 August 2026.
Fluorescent probes enable real‐time visualization of neutrophil effector functions. This review summarizes current probe design strategies and emerging tools for imaging key enzymatic activities, with emphasis on mechanisms underlying NETosis, a lytic form of neutrophil extracellular trap (NET) formation. We discuss current limitations, challenges, and
Enebie Ramos Cáceres, Marijn J. Hollander, Kimberly M. Bonger   +2 more
wiley   +1 more source

Two Lineages of DS‐1‐Like G3P[8] Human Rotaviruses in Sabah, Malaysia: Genetic Diversity and Evidence of Reassortment

Journal of Medical Virology, Volume 98, Issue 8, August 2026.
ABSTRACT Group A rotavirus (RVA) genotype G3P[8] strains typically carry a Wa‐like genomic backbone, but DS‐1‐like G3P[8] reassortants have disseminated globally since 2013. In Sabah, Malaysia, surveillance in 2018–2019 identified frequent G3P[8] strains with a short RNA electropherotype, consistent with DS‐1‐like backbones.
Yuki Akari, Lia Natasha Amit, Jecelyn Leaslie John, Daisuke Mori, Abraham Zefong Chin, Andau Konodan Mosiun, Saori Fukuda, Maurine Mumo Mutua, Ibuki Sato, Yoshimasa Maeno, Kamruddin Ahmed, Satoshi Komoto   +11 more
wiley   +1 more source

Diarrhea Induction by Rotavirus NSP4 in the Homologous Mouse Model System

Virology, 1999
Comparison of the NSP4 amino acid sequences from 31 strains of mammalian rotaviruses revealed the presence of four distinct NSP4 alleles; i.e., the Wa, KUN, AU-1, and EW alleles. The EW allele consists only of NSP4s from murine rotavirus strains and is divergent from other NSP4 alleles from the evolutionary perspective.
Horie, Y., Nakagomi, O., Koshimura, Y., Nakagomi, T., Suzuki, Y., Oka, T., Sasaki, S., Matsuda, Y., Watanabe, S.   +8 more
openaire   +2 more sources

Porcine reproductive and respiratory syndrome virus non-structural protein 4 cleaves guanylate-binding protein 1 via its cysteine proteinase activity to antagonize GBP1 antiviral effect

Veterinary Research, 2022
Porcine reproductive and respiratory syndrome (PRRS) is a highly infectious disease caused by PRRS virus (PRRSV) that causes great economic losses to the swine industry worldwide.
Hong Duan, Haoxin Dong, Shuya Wu, Jiahui Ren, Mingfang Zhang, Chuangwei Chen, Yongkun Du, Gaiping Zhang, Angke Zhang   +8 more
doaj   +1 more source