Detection of M. catarrhalis and NTHI in biofilms recovered from the chinchilla middle ear.
, 2013 Immunofluorescence image of a biofilm incubated with rabbit-anti NTHI outer membrane protein P5 (green), mouse anti-M. catarrhalis PilA (red), and stained with DAPI to label DNA (blue). A) Merged image, B) M.
Martha R. Bowers (317741) +5 more
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MKK3/6-p38 mediates EGFR-dependent inflammation induced by NTHi in middle ear epithelial cells.
, 2013 (A) Cells were incubated with NTHi with or without SB203580, and NTHi-induced NF-κB activation was measured by performing luciferase assay. (B) Cells were incubated with NTHi with or without SB203580, and mRNA expression of TNF-α, IL-1β and IL-8 was ...
Xiangbin Xu (338925) +9 more
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TLR2 is involved in the synergistic enhancement of NTHi-induced MUC5AC expression by PMA.
, 2013 (A) Overexpression of a DN mutant form of TLR2 inhibited the synergistic induction of MUC5AC transcription by NTHi and PMA in human epithelial cells, as assessed by MUC5AC-dependent promoter Luciferase assay. Cells were transfected with 0.8 µg of DN TLR2
Hirofumi Jono (35203) +3 more
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NTHi biofilms protect against a lethal dose of cefuroxime.
, 2014 Biofilms of NTHi strains 2019 and PittGG were formed overnight in the absence of antibiotic (Untreated), or in the presence of 170 ng/mL ampicillin (AMP), 230 ng/mL amoxicillin (AMOX) or 170 ng/mL cefuroxime (CEF).
Charles Wang (242565) +8 more
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Proposed model for host-mediated nutritional environment effects on NTHI pathogenesis.
, 2013 NTHI (dark green bacteria) asymptomatically colonizes the heme-iron sufficient nasopharynx epithelium (orange cells) (A). Permissive factors promote ascension up the Eustachian tube to the intact middle ear epithelium (brown cells) where NTHI are heme ...
Blake R. Szelestey (469458) +4 more
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NTHi infection suppressed systemic IL-13 and IFN-γ responses in AAD.
, 2013 Groups of mice were infected during (d0 NTHi+OVA) or 10 days after (d10 NTHi+OVA) OVA sensitization, and AAD was analyzed (on day 16). The effect of infection on IL-5 (A), IL-13 (B), and IFN-γ (C) release from splenocytes stimulated with OVA was assessed.
Peter G. Gibson (88654) +7 more
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Changing Landscape of Haemophilus influenzae Meningitis and Implication on Public Health
Case Reports in Infectious DiseasesHaemophilus influenzae (H. influenzae) has evolved as a prominent pathogen, with nontypeable strains (NTHi) emerging as a leading cause of invasive disease, particularly among the elderly. Since the introduction of Haemophilus influenzae B (Hib) vaccine,
John Allonce +4 more
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NTHi is a potent stimulator of innate inflammatory mediators regardless of susceptibility to OM.
, 2018 PBMC from otitis-prone children (cases; black dots) or non-otitis-prone children (controls; grey squares) were challenged with NTHi 86-028NP (NTHi-86), NTHi 289 or SEB and cytokine secretion was measured at 4h (left panel) or 24h (right panel) post ...
Dino Tan (5066372) +6 more
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NTHi-induced CYLD is responsible for down-regulation of inflammatory response.
, 2013 A & B, NTHi induced CYLD expression at both mRNA (A) and protein levels (B) in HMEEC-1 cells. C, NTHi induced CYLD expression at the mRNA level in the middle ear of WT mice.
Hirofumi Jono (35203) +9 more
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International Journal of Medical MicrobiologyNontypeable Haemophilus influenzae (NTHi) is the dominant pathogen in several infectious diseases. Currently the use of antibiotics is the main intervention to prevent NTHi infections, however with the emergence of drug resistant strains, it has ...
Nan Hu +5 more
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