Results 61 to 70 of about 6,490,151 (221)

Human Cytomegalovirus UL97 Phosphorylates the Viral Nuclear Egress Complex

, 2019
Herpesvirus nucleocapsids exit the host cell nucleus in an unusual process known as nuclear egress. The human cytomegalovirus (HCMV) UL97 protein kinase is required for efficient nuclear egress, which can be explained by its phosphorylation of the ...
Lye, Ming, Bender, Brian, Coen, Donald, Sharma, Mayuri, Hogle, James, Pesola, Jean, Kamil, Jeremy, Reim, Natalia   +7 more
core   +1 more source

The Oligomeric Assemblies of Cytomegalovirus Core Nuclear Egress Proteins Are Associated with Host Kinases and Show Sensitivity to Antiviral Kinase Inhibitors

Viruses, 2022
The nucleo-cytoplasmic capsid egress of herpesviruses is a unique regulated process that ensures the efficiency of viral replication and release. For human cytomegalovirus (HCMV), the core of the nuclear egress complex (NEC) consists of the pUL50–pUL53 ...
Jintawee Kicuntod, Sigrun Häge, Friedrich Hahn, Heinrich Sticht, Manfred Marschall   +4 more
doaj   +1 more source

Structure of a herpesvirus nuclear egress complex subunit reveals an interaction groove that is essential for viral replication. [PDF]

Proc Natl Acad Sci U S A, 2015
Significance Human cytomegalovirus (HCMV) is an important human pathogen. Current anti-HCMV therapies suffer from toxicities, drug resistance, and/or pharmacokinetic limitations. A possible antiviral drug target is a two-subunit complex that orchestrates nuclear egress, an essential, unusual mechanism by which nucleocapsids move from ...
Leigh KE, Sharma M, Mansueto MS, Boeszoermenyi A, Filman DJ, Hogle JM, Wagner G, Coen DM, Arthanari H.   +8 more
europepmc   +6 more sources

Generation and characterization of monoclonal antibodies specific for the Pseudorabies Virus nuclear egress complex

Virus Research, 2020
During herpesvirus replication, newly synthesized nucleocapsids exit the nucleus by a vesicle-mediated transport, which requires the nuclear egress complex (NEC), composed of the conserved viral proteins designated as pUL31 and pUL34 in the alphaherpesviruses pseudorabies virus (PrV) and herpes simplex viruses.
Julia E, Hölper, Sven, Reiche, Kati, Franzke, Thomas C, Mettenleiter, Barbara G, Klupp   +4 more
openaire   +2 more sources

Biochemical, Biophysical, and Mutational Analyses of Subunit Interactions of the Human Cytomegalovirus Nuclear Egress Complex [PDF]

Journal of Virology, 2009
ABSTRACT Nuclear egress, the trafficking of herpesvirus nucleocapsids from the nucleus to the cytoplasm, involves two conserved viral proteins that form a complex at the nuclear envelope, referred to as the nuclear egress complex. In human cytomegalovirus, these two proteins are called UL50 and UL53. To study UL50 and UL53 in molecular detail,
Sam, My, Evans, Brady, Coen, Donald, Hogle, James   +3 more
openaire   +4 more sources

Mapping of sequences in Pseudorabies virus pUL34 that are required for formation and function of the nuclear egress complex. [PDF]

J Virol, 2013
ABSTRACT The nuclear egress complex (NEC) is required for efficient translocation of newly synthesized herpesvirus nucleocapsids from the nucleus to the cytosol. It consists of the type II membrane protein pUL34 which interacts with pUL31 at the inner nuclear membrane (INM).
Paßvogel L, Trübe P, Schuster F, Klupp BG, Mettenleiter TC.   +4 more
europepmc   +4 more sources

Inhibition of Human Cytomegalovirus Particle Maturation by Activation of Liver X Receptor

Frontiers in Microbiology, 2022
Human cytomegalovirus (HCMV), a herpesvirus family member, is a large, complex enveloped virus. The activation of liver X receptor (LXR) can significantly inhibit the replication of HCMV and weaken the virulence of progeny virus (unpublished data).
Bingnan Liu, Bingnan Liu, Bingnan Liu, Yanping Ma, Yanping Ma, Yanping Ma, Yujing Huang, Yujing Huang, Yujing Huang, Zhongyang Liu, Zhongyang Liu, Zhongyang Liu, Qiang Ruan, Qiang Ruan, Qiang Ruan, Ying Qi, Ying Qi, Ying Qi   +17 more
doaj   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source