Results 111 to 120 of about 8,068,483 (291)

The Nuclear Pore Complex: A Target for NS3 Protease of Dengue and Zika Viruses

Viruses, 2020
During flavivirus infection, some viral proteins move to the nucleus and cellular components are relocated from the nucleus to the cytoplasm. Thus, the integrity of the main regulator of the nuclear-cytoplasmic transport, the nuclear pore complex (NPC ...
Luis Adrián De Jesús-González, Margot Cervantes-Salazar, José Manuel Reyes-Ruiz, Juan Fidel Osuna-Ramos, Carlos Noe Farfán-Morales, Selvin Noé Palacios-Rápalo, José Humberto Pérez-Olais, Carlos Daniel Cordero-Rivera, Arianna M. Hurtado-Monzón, Fernando Ruíz-Jiménez, Ana Lorena Gutiérrez-Escolano, Rosa María del Ángel   +11 more
doaj   +1 more source

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Simple biophysics underpins collective conformations of the intrinsically disordered proteins of the Nuclear Pore Complex

eLife, 2016
Nuclear Pore Complexes (NPCs) are key cellular transporter that control nucleocytoplasmic transport in eukaryotic cells, but its transport mechanism is still not understood.
Andrei Vovk, Chad Gu, Michael G Opferman, Larisa E Kapinos, Roderick YH Lim, Rob D Coalson, David Jasnow, Anton Zilman   +7 more
doaj   +1 more source

Functional comparison of EncB and EncC cargo proteins in iron storage within the Myxococcus xanthus encapsulin

FEBS Letters, EarlyView.
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell, Thomas Walther, Egbert Hoiczyk   +2 more
wiley   +1 more source

‘Guide and Prejudice’— How Argonautes recognize targets across domains of life

FEBS Letters, EarlyView.
Argonaute proteins use short nucleic‐acid guides to locate and regulate specific targets across all domains of life. Despite striking diversity—from human gene silencing to bacterial immune defence—all Argonautes share a conserved three‐stage recognition logic: guide‐directed sampling, progressive target pairing with a conformational checkpoint and ...
Jack P. K. Bravo
wiley   +1 more source

Crystal Structure of the Herpesvirus Nuclear Egress Complex Provides Insights into Inner Nuclear Membrane Remodeling

Cell Reports, 2015
Although nucleo-cytoplasmic transport is typically mediated through nuclear pore complexes, herpesvirus capsids exit the nucleus via a unique vesicular pathway.
Tzviya Zeev-Ben-Mordehai, Marion Weberruß, Michael Lorenz, Juliana Cheleski, Teresa Hellberg, Cathy Whittle, Kamel El Omari, Daven Vasishtan, Kyle C. Dent, Karl Harlos, Kati Franzke, Christoph Hagen, Barbara G. Klupp, Wolfram Antonin, Thomas C. Mettenleiter, Kay Grünewald   +15 more
doaj   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Nuclear positioning and functional regulation of endogenous genes and transgenes in the fruit fly Drosophila melanogaster and in mammalian cells [PDF]

, 2006
The goal of the work was to address the role of higher order nuclear architecture in the functional regulation of endogenous genes and transgenes in different species.
Rybakina, Elena
core   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

ZC3HC1 has many functions distinct from TPR and is dispensable for TPR localisation to the nuclear basket [version 2; peer review: 1 approved, 4 approved with reservations]

Wellcome Open Research
Background The nuclear basket is a ‘fishtrap’-like structure on the nucleoplasmic face of the nuclear pore complex which has been implicated in diverse functions including RNA export, heterochromatin organisation, and mitosis. Recently, a novel component
Wendy A Bickmore, Juan Carlos Acosta, Bethany M Bartlett   +2 more
doaj   +1 more source