A new approach for detecting HPV DNA in cervical swabs: comparison of nucleic acid extraction with direct PCR. [PDF]
Virol JBackground Almost all cases of cervical cancer are associated with persistent high-risk HPV infection. WHO prioritizes primary HPV testing for cervical cancer screening.
Çubuk F +6 more
europepmc +3 more sources
Unlocked nucleic acid (UNA) and UNA derivatives: thermal denaturation studies. [PDF]
Nucleic Acids Symposium Series, 2008 A study on the thermal stability of duplexes formed between unlocked nucleic acid (UNA) modified DNA and RNA oligonucleotides and complementary DNA and RNA is presented. The acyclic UNA monomers are shown to induce a decrease in duplex thermal stability.
T. B. Jensen, Niels Langkjaer, J. Wengel
semanticscholar +6 more sources
Computer programs for analysis of nucleic acid hybridization, thermal denaturation, and gel electrophoresis data. [PDF]
Nucleic Acids Research, 1979 Computer programs for the analysis of data from techniques frequently used in nucleic acids research are described. In addition to calculating non-linear, least-squares solutions to equations describing these systems, the programs allow for data editing, normalization, plotting and storage, and are flexible and simple to use.
R. F. Murphy, W. Pearson, J. Bonner
semanticscholar +7 more sources
Cytofluorometric studies on conformation of nucleic acids in situ. II. Denaturation of deoxyribonucleic acid. [PDF]
Journal of Histochemistry & Cytochemistry, 1976 Thermal denaturation of deoxyribonucleic acid (DNA) in situ in individual unbroken cells is studied by a cytofluorometric method. This method allows us to investigate DNA denaturation in the presence of divalent cations at concentrations reported to be necessary to maintain native structure of nuclear chromatin.
Z. Darżynkiewicz +4 more
semanticscholar +5 more sources
Applied Sciences, 2022 Peptide nucleic acid (PNA) is an analog of natural nucleic acids, where the sugar-phosphate backbone of DNA is replaced by an electrostatically neutral N-(2-aminoethyl)glycine backbone.
Yuichiro Aiba +2 more
doaj +2 more sources
An improved nucleic acid sequence-based amplification method mediated by T4 gene 32 protein.
PLoS ONE, 2022 The uptake of Nucleic Acid Sequence-Based Amplification (NASBA) for point of care testing may be hindered by a complexity in the workflow due the requirement of a thermal denaturation step to initiate the cyclic isothermal amplification before the ...
Yi Heng Nai +2 more
doaj +2 more sources
Denaturation of proteins and nucleic acids by thermal-gradient electrophoresis [PDF]
Biochemical Journal, 1981 A polyacrylamide-gel-electrophoresis method has been developed that permits the analysis of conformational changes that occur during the thermal denaturation of macromolecules. A stable transverse temperature gradient was produced in an aluminium heating jacket clamped around a vertical polyacrylamide slab gel.
D R Thatcher, B A Hodson
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Synthesis and Evaluation of Artificial Nucleic Acid Bearing an Oxanorbornane Scaffold
Molecules, 2020 Natural oligonucleotides have many rotatable single bonds, and thus their structures are inherently flexible. Structural flexibility leads to an entropic loss when unwound oligonucleotides form a duplex with single-stranded DNA or RNA.
Hibiki Komine +8 more
doaj +2 more sources
Denaturation and condensation of intracellular nucleic acids monitored by fluorescence depolarization of intercalating dyes in individual cells. [PDF]
Journal of Histochemistry & Cytochemistry, 1989 The intercalating binding of planar aromatic dye molecules to nucleic acids can be analyzed using fluorescence depolarization measurements of the dye molecules excited by linearly polarized light. In this study, we investigated the conformational changes of the intracellular DNA-dye complex in single cells.
Wolfgang Beisker, W.G. Eisert
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Computer programs to assist in high resolution thermal denaturation and circular dichroism studies on nucleic acids [PDF]
Nucleic Acids Research, 1981 Computer programs are described that direct the collection, processing, and graphical display of numerical data obtained from high resolution thermal denaturation (1-3) and circular dichroism (4) studies. Besides these specific applications, the programs may also be useful, either directly or as programming models, in other types of spectrophotometric ...
Thomas C. Goodman +3 more
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