Results 1 to 10 of about 117 (115)
Fast and non-toxic in situ hybridization without blocking of repetitive sequences. [PDF]
PLoS ONE, 2012 Formamide is the preferred solvent to lower the melting point and annealing temperature of nucleic acid strands in in situ hybridization (ISH). A key benefit of formamide is better preservation of morphology due to a lower incubation temperature. However,
Steen H Matthiesen, Charles M Hansen
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Applied Sciences, 2022 Peptide nucleic acid (PNA) is an analog of natural nucleic acids, where the sugar-phosphate backbone of DNA is replaced by an electrostatically neutral N-(2-aminoethyl)glycine backbone.
Yuichiro Aiba +2 more
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Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification
Micromachines, 2023 The polymerase chain reaction (PCR) is essential in nucleic acid amplification tests and is widely used in many applications such as infectious disease detection, tumor screening, and food safety testing; however, most PCR devices have inefficient ...
Yi-Quan An +10 more
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Unlocked nucleic acid (UNA) and UNA derivatives: Thermal denaturation studies [PDF]
Nucleic Acids Symposium Series, 2008 A study on the thermal stability of duplexes formed between unlocked nucleic acid (UNA) modified DNA and RNA oligonucleotides and complementary DNA and RNA is presented. The acyclic UNA monomers are shown to induce a decrease in duplex thermal stability.
Jensen, Troels Bundgaard +2 more
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Journal of Advanced Veterinary and Animal Research, 2020 Objective: The study of an in vitro embryosis is crucial in genetics for breed improvement and reproduction in livestock, identifying the causes of infertility, and stem cell application.
Dae Seung Kim +2 more
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Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion Efficiency
Applied Sciences, 2020 Peptide nucleic acid can recognise sequences in double-stranded DNA (dsDNA) through the formation of a double-duplex invasion complex. This double-duplex invasion is a promising method for the recognition of dsDNA in cellula because peptide nucleic acid (
Yuichiro Aiba +3 more
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Individual Case Analysis of Postmortem Interval Time on Brain Tissue Preservation. [PDF]
PLoS ONE, 2016 At autopsy, the time that has elapsed since the time of death is routinely documented and noted as the postmortem interval (PMI). The PMI of human tissue samples is a parameter often reported in research studies and comparable PMI is preferred when ...
Jeffrey A Blair +11 more
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Single-cell analysis reveals microbial spore responses to microwave radiation
Journal of Innovative Optical Health Sciences, 2023 To determine the effects of microwave radiation at the molecular level as well as on the germination, growth and morphology of dry spores at the single-cell level.
Siyi Qiu, Haihua Fan, Lin He
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Denaturation of proteins and nucleic acids by thermal-gradient electrophoresis [PDF]
Biochemical Journal, 1981 A polyacrylamide-gel-electrophoresis method has been developed that permits the analysis of conformational changes that occur during the thermal denaturation of macromolecules. A stable transverse temperature gradient was produced in an aluminium heating jacket clamped around a vertical polyacrylamide slab gel.
D R, Thatcher, B, Hodson
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Physiological and biochemical responses of Yarrowia lipolytica to dehydration induced by air-drying and freezing. [PDF]
PLoS ONE, 2014 Organisms that can withstand anhydrobiosis possess the unique ability to temporarily and reversibly suspend their metabolism for the periods when they live in a dehydrated state.
Caroline Pénicaud +6 more
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